A graphene oxide-based FRET sensor for rapid and sensitive detection of matrix metalloproteinase 2 in human serum sample

Song, Erqun; Cheng, Dan; Song, Yang; Jiang, Mingdong; Yu, Jiguo; Wang, Yunyun

doi:10.1016/j.bios.2013.03.030

Cited by 94 publications

(47 citation statements)

References 28 publications

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“…in vivo (Akers et al 2012) and in vitro (Li et al 2015). Based on the specific cleavage site of MMP2 between the Leu and Gly of Pro-Leu-Gly-Val-Arg, some nanoprobes via engineering a dye-labeled peptide (Pep-dye) containing the MMP2-specific cleavage substrate onto the surface of nanoparticle have been fabricated for sensitive MMP2 detection Li et al 2011;Feng et al 2011;Song et al 2013;Xi et al 2016). Wang and coworkers (2014) used poly(m-phenylenediamine) (PMPD) as an acceptor to develop PMPD-Pep-FITC probe showing 32 pM of detection limit for MMP2 .…”

Section: Electronic Supplementary Materialsmentioning

confidence: 99%

“…Wang and coworkers (2014) used poly(m-phenylenediamine) (PMPD) as an acceptor to develop PMPD-Pep-FITC probe showing 32 pM of detection limit for MMP2 . Using the good optical absorptivity of graphene oxide (GO), Song and coworkers developed a GO-Pep-FITC probe that could detect MMP2 at concentration as low as 35 pM (Song et al 2013). Considering the higher optical absorptivity of reduced graphene oxide (rGO) over GO, we recently developed a non-covalent rGO-based FRET nanoprobe (nrGO/Pep-FITC) with 3 pM of detection limit for MMP2 detection (Xi et al 2016).…”

Section: Electronic Supplementary Materialsmentioning

confidence: 99%

“…Preparation of nrGO-Pep-FITC nanoprobe was performed and stored just as described previously (Song et al 2013). nrGO was dispersed in borate buffer.…”

Section: Preparation Of Nrgo-pep-fitc Nanoprobementioning

confidence: 99%

“…Effects of BSA and surfactant on nrGO-Pep-FITC were evaluated just as described previously (Song et al 2013). …”

Section: Stability Of Nrgo-pep-fitc Nanoprobementioning

confidence: 99%

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Covalent linking peptide to hydrothermally reduced graphene oxide for ultrasensitive detection of matrix metalloproteinase 9

Lin

et al. 2017

Appl Nanosci

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Exact assay of matrix metalloproteinase 9 (MMP9) has attracted considerable attentions for the clinical diagnosis of disease in early stage. In this report, we covalently engineer a fluorescein isothiocyanate-labeled peptide (Pep-FITC) linker containing the specific cleavage substrate of MMP9 onto the surface of hydrothermally reduced nano-graphene oxide (nrGO) to develop a FRETbased nrGO-Pep-FITC nanoprobe for ultrasensitive detection of MMP9. Upon cleavage of the Pep-FITC at the amide bond between Ser and Leu by MMP9, FITC was separated from nrGO, and fluorescence recovery of FITC was proportional to the MMP9 concentration within 0.01-0.06 nM and 0.06-0.15 nM ranges, respectively, in an aqueous solution, exhibiting 0.83 pM of detection limit. This nanoprobe is stable under borate buffer (pH = 7.4) with bovine serum albumin or 0.1% surfactant Triton 100 or Tween 20, and has good specificity for MMP9 detection, which is meaningful for MMP9-related clinical and bioanalytical applications.

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Section: Electronic Supplementary Materialsmentioning

confidence: 99%

Section: Electronic Supplementary Materialsmentioning

confidence: 99%

“…Preparation of nrGO-Pep-FITC nanoprobe was performed and stored just as described previously (Song et al 2013). nrGO was dispersed in borate buffer.…”

Section: Preparation Of Nrgo-pep-fitc Nanoprobementioning

confidence: 99%

“…Effects of BSA and surfactant on nrGO-Pep-FITC were evaluated just as described previously (Song et al 2013). …”

Section: Stability Of Nrgo-pep-fitc Nanoprobementioning

confidence: 99%

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Covalent linking peptide to hydrothermally reduced graphene oxide for ultrasensitive detection of matrix metalloproteinase 9

Lin

et al. 2017

Appl Nanosci

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“…[19][20][21][22] Many kinds of GO-based FRET sensors have been developed for sensitive MMP2 detection. [23][24][25] Reduced graphene oxide (rGO) is a more effective quencher due to the higher ability to absorb visible light over GO. [26][27][28] There are many ways to prepare rGO by using strong alkaline, alcohols, natural extracts, or overheated supercritical water in sealed container as reductant.…”

mentioning

confidence: 99%

A reduced graphene oxide-based fluorescence resonance energy transfer sensor for highly sensitive detection of matrix metalloproteinase 2

Xi¹,

Wang²,

Chen³

2016

IJN

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A novel fluorescence nanoprobe (reduced nano-graphene oxide [nrGO]/fluorescein isothiocyanate-labeled peptide [Pep-FITC]) for ultrasensitive detection of matrix metalloproteinase 2 (MMP2) has been developed by engineering the Pep-FITC comprising the specific MMP2 substrate domain (PLGVR) onto the surface of nrGO particles through non-covalent linkage. The nrGO was obtained by water bathing nano-graphene oxide under 90°C for 4 hours. After mixing the nrGO and Pep-FITC for 30 seconds, the fluorescence from Pep-FITC was almost completely quenched due to the fluorescence resonance energy transfer between fluorescein isothiocyanate (FITC) and nrGO. Upon cleavage of the amide bond between Leu and Gly in the Pep-FITC by protease-MMP2, the FITC bound to nrGO was separated from nrGO surface, disrupting the fluorescence resonance energy transfer process and resulting in fluorescence recovery of FITC. Under optimal conditions, the fluorescence recovery of nrGO/Pep-FITC was found to be directly proportional to the concentration of MMP2 within 0.02–0.1 nM. The detection limit of the nrGO/Pep-FITC was determined to be 3 pM, which is approximately tenfold lower than that of the unreduced carboxylated nano-graphene oxide/Pep-FITC probe.

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Graphene‐based Nanosystems for the Detection of Proteinic Biomarkers of Disease

Menaa

Vashist

Abdelghani

et al. 2016

Wireless Computing in Medicine

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A graphene oxide-based FRET sensor for rapid and sensitive detection of matrix metalloproteinase 2 in human serum sample

Cited by 94 publications

References 28 publications

Covalent linking peptide to hydrothermally reduced graphene oxide for ultrasensitive detection of matrix metalloproteinase 9

Covalent linking peptide to hydrothermally reduced graphene oxide for ultrasensitive detection of matrix metalloproteinase 9

A reduced graphene oxide-based fluorescence resonance energy transfer sensor for highly sensitive detection of matrix metalloproteinase 2

Graphene‐based Nanosystems for the Detection of Proteinic Biomarkers of Disease

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