A Greenhouse Method to Screen Brachiariagrass Genotypes for Aluminum Resistance and Root Vigor

Abstract

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Evaluation of aluminum resistance in hybrids of Brachiaria decumbens Stapf.

“…These results show that hybrids with excellent root systems in the absence of Al can be expected when compared to the commercial cultivar, such as hybrids R151 and R137 (right superior side of Figure 1). Wenzl et al (2006) evaluated 38 interspecific hybrids, together with parental genotypes Basilisk and B. ruziziensis clone 44-02, also identified several hybrids with positive transgressive segregation, which suggests presence of heterosis for this trait. High accuracy (0.83) was obtained in the evaluation of hybrid root vigor, denoting good overall precision.…”

“…In the case of Al resistance, the number of replications used (three) guaranteed a moderate experimental precision, but not as high as desirable (accuracy above 0.70), which could also have contributed for a lower heritability estimate amongst progeny means (Table 1). Wenzl et al (2006), evaluating 38 interspecific hybrids (B. ruziziensis x B. decumbens), measured the total root length by scanning stained roots, and analyzed the images with the WinRIZHO software (Régent Instruments Inc., Québec, Canada). Hybrids were then evaluated in 10 subgroups of experiments.…”

“…Forty-five days after potting, vegetative propagules were produced from tillers with three to five leaves and up to three nodes. The tillers were detached below the lowest node above soil level and transplanted into a nutrient solution in greenhouse for rooting, as described by Wenzl et al (2006).…”

“…One propagule of each of three pairs was transferred to Solution 1 (200 mM CaCl2,pH 4.20), and the other, to Solution 2 (200 mM CaCl2, 200 mM AlCl3, pH 4.20), as described by Wenzl et al (2006). The pH of both solutions was adjusted to 4.2 by adding calculated quantities of HCl and measured with a pH electrode.…”

“…Basilisk proved to be the most Al-resistant (Wenzl et al 2001). Wenzl et al (2006) developed a methodology to evaluate the edaphic adaptation of brachiaria grass genotypes using vegetative propagules (stem cuttings) grown in solution culture. The procedure was designed to quantify two key components: root vigor and Al resistance.…”