A GSH Fluorescent Probe with a Large Stokes Shift and Its Application in Living Cells

Mao, Yueyuan; Xu, Yediao; Li, Zhi; Wang, Yan; Du, Huanhuan; Liu, Lei; Ding, Ran; Liu, Guodong

doi:10.3390/s19245348

Cited by 7 publications

(5 citation statements)

References 36 publications

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“…3b, the emission intensity (586 nm) was linearly proportional to the GSH concentration in the range of 0–20 μM ( R = 0.99355), which demonstrated that MITO-PQDNs had the potential ability to detect GSH quantitatively. Compared with other reported probes for GSH detection, 32–36 MITO-PQDNs had higher sensitivity for GSH with a detection limit as low as 253 nM.…”

Section: Resultsmentioning

confidence: 84%

A water-soluble fluorescent probe for monitoring mitochondrial GSH fluctuations during oxidative stress

Wang

Zhang²,

Jin³

et al. 2022

RSC Adv.

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Section: Resultsmentioning

confidence: 84%

A water-soluble fluorescent probe for monitoring mitochondrial GSH fluctuations during oxidative stress

Wang

Zhang²,

Jin³

et al. 2022

RSC Adv.

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“…Previous research has emphasized MDA as an indicator of lipid peroxidation while suggesting that SOD is an enzyme that can eliminate harmful substances produced in the process of oxidative metabolism ( Wang et al, 2019 ). GSH is the most abundant non-protein biothiol and is a central antioxidant to defend against the effects of toxins and free radicals in aging ( Mao et al, 2019 ). 5-HT is a neurotransmitter derived from tryptophan that is synthesized both centrally and systemically ( Sahu et al, 2018 ).…”

Section: Discussionmentioning

confidence: 99%

miR-96 Inhibits SV2C to Promote Depression-Like Behavior and Memory Disorders in Mice

Sun¹,

Bai²,

Lin³

et al. 2021

Front. Behav. Neurosci.

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Accumulating evidence continues to emphasize the role of microRNAs as significant contributors to depression-like behavior and memory disorders. The current study aimed to investigate the mechanism by which miR-96 influences depression-like behavior and memory deficit in mice. A depression-like behavior and memory disorder mouse model was initially established by means of intraperitoneal injection with lipopolysaccharide. Memory deficits in the mice were evaluated using the Novel Object Recognition Test and Morris water maze experiments, whereas the Sucrose Preference Experiment and forced swimming experiments were performed to identify depression-like behavior in mice. The levels of tumor necrosis factor-α, malondialdehyde, superoxide dismutase, glutathione, and the monoamine transmitters 5-hydroxytryptamine and dopamine were subsequently detected in the serum. Reverse transcription-quantitative polymerase chain reaction and Western blot analysis evaluated the expression of miR-96 and SV2C expression in the CA1 hippocampal region of the mice. Finally, the relationship of miR-96 and SV2C was verified by dual-luciferase reporter gene assay. Our data indicated that the expression of miR-96 was increased, whereas that of SV2C was decreased in the CA1 region of mice exhibiting depression-like behavior and memory impairment. When miR-96 was downregulated or SV2C was overexpressed via intra-cerebroventricular injection with a miR-96 antagonist (miR-96 antagomir) or overexpression of SV2C vector, the Novel Object Recognition Test and sucrose preference index were increased, whereas the escape latency, the number of water maze platform crossings, and the immobility time of the mice were decreased. The serum levels of tumor necrosis factor-α, interleukin-1β, and malondialdehyde in the mouse CA1 region of mice were reduced, whereas the levels of superoxide dismutase and glutathione were elevated after the downregulation of miR-96 or overexpression of SV2C. Collectively, our study demonstrates that miR-96 negatively regulates the expression of SV2C, which consequently leads to depression-like behavior and memory impairment in mice. Our findings highlight the potential of miR-96-targeted therapeutics.

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“…Both of the above designs introduce a strong electron-absorbing group into the probe structure, and the fluorescence of probe is quenched or resurrected after GSH regulation. There are also some references for the application of this design [103][104][105][106][107][108][109].…”

Section: Fluorescent Nano-probe Design Based On Physiological Properties Of Gshmentioning

confidence: 99%

Nano-Drug Design Based on the Physiological Properties of Glutathione

2021

Molecules

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Glutathione (GSH) is involved in and regulates important physiological functions of the body as an essential antioxidant. GSH plays an important role in anti-oxidation, detoxification, anti-aging, enhancing immunity and anti-tumor activity. Herein, based on the physiological properties of GSH in different diseases, mainly including the strong reducibility of GSH, high GSH content in tumor cells, and the NADPH depletion when GSSH is reduced to GSH, we extensively report the design principles, effect, and potential problems of various nano-drugs in diabetes, cancer, nervous system diseases, fluorescent probes, imaging, and food. These studies make full use of the physiological and pathological value of GSH and develop excellent design methods of nano-drugs related to GSH, which shows important scientific significance and prominent application value for the related diseases research that GSH participates in or responds to.

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A GSH Fluorescent Probe with a Large Stokes Shift and Its Application in Living Cells

Cited by 7 publications

References 36 publications

A water-soluble fluorescent probe for monitoring mitochondrial GSH fluctuations during oxidative stress

A water-soluble fluorescent probe for monitoring mitochondrial GSH fluctuations during oxidative stress

miR-96 Inhibits SV2C to Promote Depression-Like Behavior and Memory Disorders in Mice

Nano-Drug Design Based on the Physiological Properties of Glutathione

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