A Guide Inside Electrochemiluminescent Microscopy Mechanisms for Analytical Performance Improvement

Recent progress in electrochemiluminescence microscopy analysis of single cells

“…2 Since ECLM has been gradually becoming a powerful tool in the fundamental studies of ECL mechanisms and biological applications, an increasing number of researchers have used ECLM to investigate traditional or new ECL systems, which in turn promotes the development of ECLM. 10 Cellular analysis is becoming increasingly important as we step into the age of life science. 11,12 Thus, many advanced techniques have been developed to investigate relevant information about cells.…”

“…Each of these routes has its own features when used to design single-cell ECLM. We also recommend readers to refer to relevant reviews involving single-cell ECLM from other perspectives, 9,10,18,19,21 which inspired us to see the bigger picture of this field.…”

“…2 Since ECLM has been gradually becoming a powerful tool in the fundamental studies of ECL mechanisms and biological applications, an increasing number of researchers have used ECLM to investigate traditional or new ECL systems, which in turn promotes the development of ECLM. 10…”

Photoswitchable CRISPR/Cas12a-Amplified and Co3O4@Au Nanoemitter Based Triple-Amplified Diagnostic Electrochemiluminescence Biosensor for Detection of miRNA-141

“…Among them, ECL has captivated increasingly deliberation and brilliance owing to the minimal background noise, ultrahigh sensitivity, extremely low detection limit, and admirable electrochemical performance and has become an indispensable part in the areas of clinical medicine, food security, environmental monitoring, and so on. In the past few decades, ECL luminophores have been cultivated from luminol, Ru­(bpy) 3 2+ , and other classical reagents to quantum dots and metal nanoclusters . To substantially exaggerate the transmission electronically intensity of luminophores, penalize the disadvantages of ambiguous registration, and escape the quite hazards of co-reaction reagents, the competitive co-reaction projectors (including aniline substances, metal complexes, noble metal nanoparticles, and semiconductor nanomaterials , ) are familiarized with the conventional binary ECL system, which can trigger a qualitative leap in signal enhancement.…”

“…In the past few decades, ECL luminophores have been cultivated from luminol, Ru(bpy) 3 2+ , and other classical reagents to quantum dots and metal nanoclusters. 13 To substantially exaggerate the transmission electronically intensity of luminophores, penalize the disadvantages of ambiguous registration, and escape the quite hazards of co-reaction reagents, 14 the competitive coreaction projectors (including aniline substances, 15 metal complexes, 16 noble metal nanoparticles, and semiconductor nanomaterials 17,18 ) are familiarized with the conventional binary ECL system, which can trigger a qualitative leap in signal enhancement. In addition to chemical enhancement factors that can manipulate the sensitivity of redox reaction, visualize powerful genetic tools and molecular machines at the biological level can also exponentially duplicate the concentration of targets at in trace standard.…”