The Niangmuco region on the east margin of the Eastern Himalayan Syntaxis features a large number of glacial lakes. The development process and stability classification of glacial lakes is of great significance to the study of seasonal glaciers in the eastern Himalayan margin, with implications for economic development and disaster prevention. Based on Landsat remote sensing image data from 2000 to 2021, this study analyzed the development and change characteristics of glacial lakes in the Niangmuco region during the past 21 years, and classified the stability of lakes with areas >0.02 km2 using the fuzzy consistent matrix method. In this area, 126 glacial lakes were identified within an elevation range of 3044–4156 m with a total area of 10.94 km2. These lakes primarily included glacial erosion lakes, valley lakes, tectonic lakes, and landslide dam lakes. Specifically, glacial erosion lakes accounted for 88.9% of the total number of lakes and 60.3% of the total lake area, followed by valley lakes with 6.3% and 23.7%, respectively. From 2000 to 2010, the total area of glacial lakes decreased from 10.53 km2 to 10.09 km2, which may be attributable to climate fluctuations. Subsequently, the area of lakes increased significantly to 10.94 km2 in 2021, an increase of 0.41 km2. Compared with 2000, among the lakes with a growth rate of 0.019 km2/a in 21 years, glacial erosion lakes exhibited the largest change. Among the classified glacial lakes in the study area, 95.7% were stable and relatively stable, mostly comprising glacial erosion lakes at high altitudes between 3468 and 4156 m. Only 4 unstable and extremely unstable glacial lakes were identified, and they were located near a fault zone. The findings show that the development and the change of glacial lakes in the area are primarily controlled by temperature and precipitation, and the topography and fault activity have important influences on the stability of glacial lakes.