A guide to preclinical evaluation of hydrogel-based devices for treatment of cartilage lesions

Karami, Peyman; Stampoultzis, Theofanis; Guo, Yanheng; Pioletti, Dominique P.

doi:10.1016/j.actbio.2023.01.015

Cited by 14 publications

(5 citation statements)

References 124 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The water uptake behavior of the developed scaffolds was analyzed, since the scaffold water uptake capacity is considered essential for tissue engineering applications due to the need to transport body fluids with nutrients to cells [21,44]. Importantly, all scaffolds maintained their structural integrity after immersion for 200 h. As can be seen in Figure 6, all the scaffolds reached an equilibrium water uptake of 300%, showing a capacity to absorb water and ensure the transport of nutrients inside the 3D printed scaffold.…”

Section: Water Uptake Of 3d Printed Scaffoldsmentioning

confidence: 99%

Optimization of Ink Composition and 3D Printing Process to Develop Soy Protein-Based Scaffolds

Carranza,

Tejo-Otero,

Bengoechea

et al. 2024

Gels

View full text Add to dashboard Cite

Inks based on soybean protein isolate (SPI) were developed and their formulations were optimized as a function of the ink heat treatment and the content of other biopolymers to assess the effects of protein–polysaccharides and protein–protein interactions. First, the rheological behavior of the inks was analyzed in relation to the polyvinyl alcohol (PVA) concentration employed (20, 25, and 30 wt%) and, as a result of the analysis, the ink with 25 wt% PVA was selected. Additionally, sodium alginate (SA) and gelatin (GEL) were added to the formulations to improve the viscoelastic properties of the inks and the effect of the SA or GEL concentrations (1, 2, and 3 wt%) was studied. All inks showed shear thinning behavior and self-supporting abilities. Among all the 3D printed scaffolds, those with higher SA (3 wt%) or GEL (2 and 3 wt%) content showed higher shape fidelity and were selected for further characterization. Texture profile analysis demonstrated that the scaffolds prepared with previously heat-treated inks containing 3 wt% GEL showed the highest strength. Additionally, these scaffolds showed a higher water-uptake capacity profile.

show abstract

Section: Water Uptake Of 3d Printed Scaffoldsmentioning

confidence: 99%

Optimization of Ink Composition and 3D Printing Process to Develop Soy Protein-Based Scaffolds

Carranza,

Tejo-Otero,

Bengoechea

et al. 2024

Gels

View full text Add to dashboard Cite

show abstract

“…In vivo tests represent a determinant step for hydrogel applications. Interdisciplinary studies for hydrogel design, characterization, and in vitro investigations [55,92,116,189,380,381], along with access to preclinical and clinical studies to ensure safety and efficacy of new materials [98,271,[382][383][384][385], will accelerate the application of new concepts and revolutionary materials. Last but not least, education regarding the protection of nature, which generously offers us a variety of biomolecules, must be promoted beyond the academic framework.…”

Section: Conclusion and Future Outlookmentioning

confidence: 99%

Diversity of Bioinspired Hydrogels: From Structure to Applications

Lupu¹,

Grădinaru²,

Gradinaru

et al. 2023

Gels

View full text Add to dashboard Cite

Hydrogels are three-dimensional networks with a variety of structures and functions that have a remarkable ability to absorb huge amounts of water or biological fluids. They can incorporate active compounds and release them in a controlled manner. Hydrogels can also be designed to be sensitive to external stimuli: temperature, pH, ionic strength, electrical or magnetic stimuli, specific molecules, etc. Alternative methods for the development of various hydrogels have been outlined in the literature over time. Some hydrogels are toxic and therefore are avoided when obtaining biomaterials, pharmaceuticals, or therapeutic products. Nature is a permanent source of inspiration for new structures and new functionalities of more and more competitive materials. Natural compounds present a series of physico-chemical and biological characteristics suitable for biomaterials, such as biocompatibility, antimicrobial properties, biodegradability, and nontoxicity. Thus, they can generate microenvironments comparable to the intracellular or extracellular matrices in the human body. This paper discusses the main advantages of the presence of biomolecules (polysaccharides, proteins, and polypeptides) in hydrogels. Structural aspects induced by natural compounds and their specific properties are emphasized. The most suitable applications will be highlighted, including drug delivery, self-healing materials for regenerative medicine, cell culture, wound dressings, 3D bioprinting, foods, etc.

show abstract

“…Hydrogels are a class of bioscaffold that can form 3-dimensional networks that possess clinically desirable properties of biocompatibility, bioadhesiveness, and biodegradability [4][5][6]. Additionally, hydrogels can be delivered as a liquid solution to fill irregularly shaped defects [7,8].…”

Section: Introductionmentioning

confidence: 99%

Enhanced osteochondral repair by leukocyte-depleted platelet-rich plasma in combination with adipose-derived mesenchymal stromal cells encapsulated in a three-dimensional photocrosslinked injectable hydrogel in a rabbit model

Iseki,

Rothrauff,

Kihara

et al. 2024

Stem Cell Res Ther

View full text Add to dashboard Cite

Introduction Intra-articular injection of adipose-derived mesenchymal stromal cells (ASCs) and/or platelet-rich plasma (PRP) have been reported to independently and synergistically improve healing of osteochondral lesions in animal models. However, their independent and combined effects when localized to an osteochondral lesion by encapsulation within a photocrosslinkable methacrylated gelatin hydrogel (GelMA) have not been explored. Herein we investigated a unique combination of allogeneic ASCs and PRP embedded in GelMA as a single-stage treatment for osteochondral regeneration in a rabbit model. Methods Thirty mature rabbits were divided into six experimental groups: (1) Sham; (2) Defect; (3) GelMA; (4) GelMA + ASCs; (5) GelMA + PRP; and (6) GelMA + ASCs + PRP.At 12 weeks following surgical repair, osteochondral regeneration was assessed on the basis of gross appearance, biomechanical properties, histological and immunohistochemical characteristics, and subchondral bone volume. Results In terms of mechanical property reflecting the ability of neotissue to bear stress, PRP only group were significantly lower than the Sham group (p = 0.0098). On the other hand, ASCs only and ASCs combined with PRP groups did not exhibit significantly difference, which suggesting that incorporation of ASCs assists in restoring the ability of the neotissue to bear stresses similarly to native tissue (p = 0.346, p = 0.40, respectively). Safranin O in ASCs combined with PRP group was significantly higher than the Defect and GelMA only groups (p = 0.0009, p = 0.0017, respectively). Additionally, ASCs only and ASCs combined with PRP groups presented especially strong staining for collagen type II. Surprisingly, PRP only and PRP + ASCs groups tended to exhibit higher collagen type I and collagen type X staining compared to ASCs only group, suggesting a potential PRP-mediated hypertrophic effect. Conclusion Regeneration of a focal osteochondral defect in a rabbit model was improved by a single-stage treatment of a photocrosslinked hydrogel containing allogenic ASCs and autologous PRP, with the combination of ASCs and PRP producing superior benefit than either alone. No experimental construct fully restored all properties of the native, healthy osteochondral unit, which may require longer follow-up or further modification of PRP and/or ASCs characteristics.

show abstract

A guide to preclinical evaluation of hydrogel-based devices for treatment of cartilage lesions

Cited by 14 publications

References 124 publications

Optimization of Ink Composition and 3D Printing Process to Develop Soy Protein-Based Scaffolds

Optimization of Ink Composition and 3D Printing Process to Develop Soy Protein-Based Scaffolds

Diversity of Bioinspired Hydrogels: From Structure to Applications

Enhanced osteochondral repair by leukocyte-depleted platelet-rich plasma in combination with adipose-derived mesenchymal stromal cells encapsulated in a three-dimensional photocrosslinked injectable hydrogel in a rabbit model

Contact Info

Product

Resources

About