A hand-powered microfluidic system for portable and low-waste sample discretization

Xie, Tengbao; Wang, Ping; Wu, Lei; Sun, Bangyong; Zhao, Qiang; Li, Gang

doi:10.1039/d1lc00448d

Cited by 21 publications

(8 citation statements)

References 45 publications

(51 reference statements)

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“…Like with ELISA, microwell arrays for nucleic acid detection can be loaded using a range of techniques. Flow cell loading [ 91 , 92 ], vacuum-assisted loading and oil-driven digitization [ 93 , 94 , 95 , 96 ], pressure loading [ 97 ], dispensing robots [ 98 , 99 ], and self-induced partitioning via SlipChip [ 100 , 101 , 102 ] have all been used for loading microwell arrays for nucleic acid applications.…”

Section: Digital Biosensing For Nucleic Acidsmentioning

confidence: 99%

Recent Advances in Digital Biosensing Technology

et al. 2022

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Digital biosensing assays demonstrate remarkable advantages over conventional biosensing systems because of their ability to achieve single-molecule detection and absolute quantification. Unlike traditional low-abundance biomarking screening, digital-based biosensing systems reduce sample volumes significantly to the fL-nL level, which vastly reduces overall reagent consumption, improves reaction time and throughput, and enables high sensitivity and single target detection. This review presents the current technology for compartmentalizing reactions and their applications in detecting proteins and nucleic acids. We also analyze existing challenges and future opportunities associated with digital biosensing and research opportunities for developing integrated digital biosensing systems.

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Section: Digital Biosensing For Nucleic Acidsmentioning

confidence: 99%

Recent Advances in Digital Biosensing Technology

et al. 2022

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“…[8,9] The digital droplet PCR (dd-PCR) technique has been employed for quantitative nucleic acid amplification for a long time due to its capability in the rapid and large-scale production of parallel PCR assays. [10][11][12][13] Several typical commercial dPCR instruments, such as Bio-Rad QX600, Stilla Naica…”

Section: Introductionmentioning

confidence: 99%

Facile prepared microfluidic chip for multiplexed digital RT‐qPCR test

Hu,

Zhu,

Tang

et al. 2023

Biotechnology Journal

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The chip‐based digital polymerase chain reaction (PCR) is an indispensable technique for amplifying and quantifying nucleic acids, which has been widely employed in molecular diagnostics at both fundamental and clinical levels. However, the previous designs have yet to achieve widespread application due to limitations in complex chip fabrication, pretreatment procedures, special surface properties, and low throughput. This study presents a facile digital microfluidic chip driven by centrifugal force for digital PCR analysis. Interestingly, regardless of the hydrophilicity or hydrophobicity of the inner chip surface, an efficient digitization process can be achieved. PCR reagents introduced into the inlet can be allocated to 9600 microchambers and subsequently isolated by the immiscible phase (silicone oil). The centrifugal priming approach offers a facile means to achieve high‐throughput analysis. The design was further employed for the quantification of nucleic acids using digital PCR. The calculated result exhibited a strong correlation with the measured value at the concentrations from 1 copy/μL to 1000 copies/μL (R2 = 0.99). Additionally, the chip also allowed digital multiplexed analysis, thereby indicating its potential for multi‐target detection applications.

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“…28 Microfluidic chip, also called Lab on a Chip (LOC), is able to achieve completely automatic operation of biological, chemical and medical analysis through integrating separation, reaction and detection into one single chip. 29,30 Centrifugal microfluidic disc, called as Lab on a Disc (LOAD), is a current research hotspot of microfluidic chip, which could automatically perform the whole biological detection procedure only through centrifugation. 31 Sequential operations of different solutions, including transfer, mixing, separation and reaction, are the key to these centrifugal microfluidic discs by simple strategies based on rotation speed, 32 rotation radius, 27 channel size 33 and slope valve angle, 34 or complex strategies based on siphon valves, 35 switch chambers 36 and other elaborate designs.…”

Section: Introductionmentioning

confidence: 99%