A headspace collection chamber for whole body volatilomics

Rankin‐Turner, Stephanie; McMeniman, Conor J.

doi:10.1039/d2an01227h

Cited by 16 publications

(24 citation statements)

References 110 publications

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“…We next leveraged our previous analyses of human whole body headspace 48 to target the primary chemical constituents of human whole body odor, confirming the identity of 40 known VOCs detected in whole body odor in this semi-field dataset (Fig. S12).…”

Section: Resultssupporting

confidence: 66%

“…The human body emits hundreds of volatile organic compounds (VOCs) in exhaled breath and skin emissions 64 , and our prior research has demonstrated considerable heterogeneity in the chemical complexity of whole body emissions between humans 48 . This natural variation in the types and abundances of VOCs emitted by the human body has long been hypothesized as a key factor driving mosquito host preference 65 .…”

Section: Discussionmentioning

confidence: 99%

“…We also observed that a skin-microbe generated compound acetoin (3-hydroxy-2-butanone) was highly enriched in the most attractive human subject in the six-choice trial. Acetoin is amongst the most abundant of VOCs detected in the human whole body volatilome 48 . The bacteria Staphylococcus epidermidis and Staphylococcus aureus have been demonstrated to produce acetoin, both of which are abundantly present on human skin 14, 73 .…”

Section: Discussionmentioning

confidence: 99%

“…Anopheles gambiae classically exhibits peak periods of host-seeking activity during the late evening and early mornings in the hours flanking midnight, when humans are typically sleeping 47 . During this time frame, humans are usually indoors, where human whole body odor inclusive of carbon dioxide (CO 2 ) and other volatile organic compounds found in skin odor and breath 48 , dissipates from the human body into the air. Human body odor rises on warm convective currents emanating from the skin to generate odor plumes that typically dissipate through the housing eaves 49 or walls to be carried downwind to provide an alluring olfactory stimulus for mosquitoes in the vicinity that indicates human presence 50–52 .…”

Section: Introductionmentioning

confidence: 99%

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A semi-field system for quantifyingAnopheles gambiaeattraction to human scent

Giraldo

Rankin‐Turner

Corver

et al. 2022

Preprint

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Variability in the chemical composition of human scent has the potential to modulate mosquito attraction to certain humans. We have engineered a large-scale, semi-field system in Zambia for quantifying mosquito olfactory preferences towards whole body odor sourced from different humans under naturalistic conditions. In a flight cage arena with infrared tracking, we document that the African malaria mosquitoAnopheles gambiaehierarchically prefers to land on heated targets mimicking human skin temperature when they are baited with carbon dioxide (CO2) over background air, human body odor over CO2, and the scent of one individual over another. In a six-choice assay configuration, we further identify humans at both ends of the attractiveness spectrum whose scent is differentially attractive toAn. gambiaerelative to other individuals. We demonstrate integrative use of this multi-choice olfactory assay with whole body volatilomics, establishing a powerful method for discovery of human odorants modulating heterogeneity in biting risk at enhanced throughput.

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Section: Resultssupporting

confidence: 66%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

A semi-field system for quantifyingAnopheles gambiaeattraction to human scent

Giraldo

Rankin‐Turner

Corver

et al. 2022

Preprint

Self Cite

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“…Even in chamber experiments where the human subjects were controlled for bathing and clothing, divergent findings have been reported regarding baseline emissions for ozonolysis products. Some studies reported low baseline emissions, ,,,− while some others suggested substantially higher levels . In real-world scenarios, the soiling conditions of skin and clothing can differ by occupants, adding extra variability to the apparent baseline emissions.…”

Section: Resultsmentioning

confidence: 99%

Emissions of Volatile Organic Compounds from Human Occupants in a Student Office: Dependence on Ozone Concentration

Qu,

Xie,

Liu

2023

ACS Environ. Au

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Human occupants themselves constitute an important source of volatile organic compounds (VOCs) in indoor environments through breath and dermal emissions. In order to quantify VOC emissions from occupants under real-world settings, previous indoor observational studies often determined emission factors (i.e., average emission rates per person). However, the values obtained across these studies exhibited large variability, and the causes of this variability still need to be understood. Herein we report 10-day real-time VOC measurements in a university student office, using a proton transfer reaction-quadrupole interface-timeof-flight mass spectrometer. A method was developed to identify VOCs of primary human origin and to quantify the corresponding emission factors, accounting for the dynamically changing occupancy level and ventilation rate in the assessed office. We found that the emission factors of many dermally emitted VOCs strongly increased as the ozone concentration increased from <3 to 10−15 ppb. These VOCs include geranyl acetone, 6-methyl-5hepten-2-one (6-MHO), and C 10 -C 12 saturated aldehydes, which align with characteristic first-generation ozonolysis products of skin oil. The strongest increase occurred for 6-MHO, from 113 to 337 μg/h/p. In comparison, acetone and isoprene, which are primarily emitted from human breath, varied little with the ozone level. In light of this finding, we conducted an integrated analysis of emission factors reported in the literature for two frequently reported species, namely, 6-MHO and decanal. Ozone concentration alone can explain 94−97% of the variation in their emission factors across previous studies, and the best-estimated ozone dependence obtained using the literature data is consistent with those obtained in the current study. These results suggest that the ozone concentration is a key factor regulating emission factors of many dermally emitted VOCs in real indoor environments, which has to be considered when reporting or using the emission factors.

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Identification of Volatile Markers in Sweat for COVID‐19 Screening by Gas Chromatography‐Mass Spectrometry

Chuachaina,

Thaveesangsakulthai,

Sinsukudomchai

et al. 2024

ChemistrySelect

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Due to an outbreak of coronavirus spreading in recent years, there is an urgent need for effective, rapid, and non‐invasive diagnostics to detect and differentiate among patients who are infected and uninfected with SARS‐CoV‐2 for controlling the virus propagation. This study developed an alternative method for COVID‐19 screening from volatile analysis of metabolites mainly studied in sweat samples obtained from the populations in Bangkok, Thailand, during December 2020 to April 2022. A total of 140 collected samples; 70 positive confirmed COVID‐19 cases and another 70 negatives confirmed COVID‐19 cases were applied to be separated, detected, and identified as volatile organic compounds (VOCs)/odor occurring from SARS‐CoV‐2 infection body by the technique of Headspace solid‐phase microextraction gas chromatography‐mass spectrometry (HS‐SPME‐GC‐MS) together with principle component analysis (PCA), which allowed potential volatile markers of COVID‐19 such as pentadecane, nonanal, styrene, xylene, ethylbenzene, and 2‐ethylhexyl acrylate (up to 94 % accuracy, 93 % sensitivity, 96 % selectivity, and 94 % specificity), respectively, along with limit of detection in a range of 0.26–125 μg. The result was validated by comparison with the RT‐PCR results.

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A headspace collection chamber for whole body volatilomics

Abstract: The human body secretes a complex blend of volatile organic compounds (VOCs) via the skin, breath and bodily fluids, the study of which can provide valuable insight into the physiological...

Cited by 16 publications

References 110 publications

A semi-field system for quantifyingAnopheles gambiaeattraction to human scent

A semi-field system for quantifyingAnopheles gambiaeattraction to human scent

Emissions of Volatile Organic Compounds from Human Occupants in a Student Office: Dependence on Ozone Concentration

Identification of Volatile Markers in Sweat for COVID‐19 Screening by Gas Chromatography‐Mass Spectrometry

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