A heat shock protein protects against oxidative stress induced by lambda-cyhalothrin in the green peach aphid Myzus persicae

Heat Shock 70 kDa Protein Cognate 3 of Brown Planthopper Is Required for Survival and Suppresses Immune Response in Plants

“…The Hsp70 family is the most influential and conserved family of heat shock proteins which exist in organisms widely [ 22 , 33 ]. Hsp70s generally produce protective tolerance to stress through molecular chaperone, antioxidant, immune defense and anti-apoptotic effects [ 34 ].…”

“…Subsequently, studies of Hsp70s in insect biology are of great help in understanding the temperature dependence on insect growth and development [ 21 ]. The knockdown of Myzus persicae heat shock protein 70 (MpHsp70) increases the susceptibility of M. persicae to lambda-cyhalothrin (LCT) [ 22 ]. In BPH, the expression level of heat shock 70 kDa protein cognate ( NlHsc70 ) in macropterous BPH remarkably increased at 32–38 °C, while the expression level of NlHSC70 in the short-wing type remained unchanged [ 23 ].…”

Epsilon Class Glutathione S-Transferase Confers Phoxim Tolerance in the Black Cutworm Agrotis ipsilon (Lepidoptera: Noctuidae)

“…A 187-bp fragment of AiGSTe1 , which was located upstream of the region for RT-qPCR analysis (Figure S2), was amplified from the pCold II- AiGSTe1 plasmid using gene-specific primers that contained T7 RNA polymerase promoter sequences ( AiGSTe1 -T7F and AiGSTe1 -T7R; Table S3). A 440-bp fragment of green fluorescent protein ( GFP ) was also amplified from the pEGFP-N1 vector (Clontech, Mountain View, CA), as described previously. , The dsRNA samples of AiGSTe1 (ds AiGSTe1 ) and GFP (ds GFP ) were synthesized using the MEGAscript T7 Transcription Kit (Thermo Fisher Scientific, Waltham, MA) and diluted to a final concentration of 2 μg/μL with nuclease-free water. Third-instar larvae of A. ipsilon were injected with a 1 μL (2 μg) volume of ds AiGSTe1 or ds GFP using a microliter syringe.…”

“…A 440-bp fragment of green fluorescent protein (GFP) was also amplified from the pEGFP-N1 vector (Clontech, Mountain View, CA), as described previously. 24,25 The dsRNA samples of AiGSTe1 (dsAiGSTe1) and GFP (dsGFP) were synthesized using the MEGAscript T7 Transcription Kit (Thermo Fisher Scientific, Waltham, MA) and diluted to a final concentration of 2 μg/μL with nuclease-free water. Third-instar larvae of A. ipsilon were injected with a 1 μL (2 μg) volume of dsAiGSTe1 or dsGFP using a microliter syringe.…”

“…A bacterial survival assay was conducted following a protocol published by the authors. 24 In brief, S2. 20 mL of a cell suspension of E. coli BL21(DE3) cells containing the pCold II-AiGSTe1 plasmid or the pCold II empty vector (control) was cultured in LB medium containing 100 mg/L ampicillin at 37 °C with shaking at 180 rpm.…”