A hemopoietic cell line dependent upon a factor in pokeweed mitogen‐stimulated spleen cell conditioning medium

Hasthorpe, Suzanne

doi:10.1002/jcp.1041050221

Cited by 51 publications

(17 citation statements)

References 12 publications

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“…Therefore, some morphologically identifiable mast cells in the peritoneal cavity may function as the mast cell precursors. Since peritoneal mast cells appear to float in the fluid, the proliferative potentiality of some peritoneal mast cells reminds us of the suspension culture system of mast cell-like cells that has been devised in many laboratories (41)(42)(43)(44)(45)(46). However, mitosis of peritoneal mast cells is seldom observed in intact animals (10).…”

Section: Discussionmentioning

confidence: 99%

Proliferation of peritoneal mast cells in the skin of W/Wv mice that genetically lack mast cells.

Sonoda¹,

Kanayama²,

Hara³

et al. 1984

The Journal of Experimental Medicine

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Presence of mast cell precursors in the mouse peritoneal cavity was demonstrated, and the precursors were characterized. When a cell suspension, containing mast cell precursor(s), was directly injected into the skin of genetically mast cell-deficient WBB6F1 (WB X C57BL/6)-W/Wv mice, a cluster composed of approximately 2,000 mast cells appeared at the injection site. By determining the proportion of injection sites at which the mast cell cluster appeared, the concentration of mast cell precursors can be calculated by limiting dilution analysis. The concentration in the peritoneal cavity was about five times as great as the concentration in the bone marrow. Although peritoneal mast cell precursors were shown to originate from the bone marrow, physical characterization revealed that the peritoneal precursors differed from the marrow precursors. The peritoneal precursors were less susceptible to irradiation than the marrow precursors; the former were heavier than the latter. When a 95% pure mast cell suspension was prepared from the peritoneal cells by the removal of phagocytes and the density gradient centrifugation, 1 out of 16 cells had the potentiality to make a mast cell cluster in the skin of the W/Wv mice. Moreover, when a single mast cell was identified under the phase contrast microscope and picked up with the micromanipulator, 1 out of 17 mast cells made the cluster. This indicated that some peritoneal mast cells kept extensive proliferative potentiality even after morphological differentiation. In other words, some peritoneal mast cells themselves may function as the committed precursors.

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Section: Discussionmentioning

confidence: 99%

Proliferation of peritoneal mast cells in the skin of W/Wv mice that genetically lack mast cells.

Sonoda¹,

Kanayama²,

Hara³

et al. 1984

The Journal of Experimental Medicine

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“…Thymic-dependent proliferation of mast cells also occurs in the mucosa of helminth-infected mice (16), and mouse hematopoietic stem cells cultured in vitro in the presence of lymphocyte conditioned medium differentiate into mast cells resembling the mucosal mast cell subclass (17)(18)(19)(20)(21)(22). The cultured cells depend on the T-cell lymphokine interleukin 3 for growth (23) and contain an oversulfated chondroitin sulfate proteoglycan, termed chondroitin sulfate E proteoglycan (24).…”

mentioning

confidence: 99%

Homology of the rat basophilic leukemia cell and the rat mucosal mast cell.

Seldin¹,

Adelman²,

Austen³

et al. 1985

Proc. Natl. Acad. Sci. U.S.A.

223

123

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Secretory granules of the rat basophilic leukemia (RBL-1) cell, a chemically-generated tumor cell line maintained in tissue culture, were shown to stain with alcian blue but not with safranin counterstain and to have sparse, small, electron-dense granules. A Mr 25,000 protein was the major [3H]diisopropyl fluorophosphate-binding protein in extracts of RBL-1 cells. Double-immunodiffusion analysis of extracts revealed immunoreactivity for rat mast cell protease (RMCP)-II, a Mr 25,000 neutral protase present in the secretory granules of rat mucosal mast cells and cultured rat bone marrow-derived mast cells, but no immunoreactivity for RMCP-I, the predominant neutral protease of rat connective tissue mast cells. By radial immunodiffusion, there was 66.8 ng of RMCP-ll per 106 cells. Whereas rat connective tissue mast cells stain with alcian blue and safranin and contain heparm proteoglycan, rat mucosal and rat bone marrow-derived mast cells stain with alcian blue only and contain a non-heparin proteoglycan and lesser amounts of histamine. Proliferation of rat mucosal mast cells in vivo and rat bone marrowderived mast cells in vitro requires T-cell factors, whereas no comparable requirement has been observed for connective tissue mast cells. The transformed RBL-1 tumor cell, whose growth is independent of factors other than those present in standard tissue culture medium, has previously been shown to contain predominantly chondroitin sulfate di-B proteoglycans and low amounts of histamine. The similar histology and secretory granule biochemistry of the rat mucosal mast cell, rat culture-derived mast cell, and RBL-1 cell suggest that they comprise a single mast cell subclass distinct from the rat connective tissue mast cell. (6)(7)(8)(9). Both of these rat mast cell subclasses can be activated with IgE and specific antigen. The connective tissue cell also responds to compound 48/80, (a condensation product of N-methyl-pmethoxyphenethylamine and formaldehyde), whereas the mucosal mast cell in situ (10) or isolated from intestine (11) does not. Disodium cromoglycate and theophylline inhibit the activation of the connective tissue mast cell but not of the mucosal mast cell (12). Homogeneous populations of mast cells resembling the mucosal mast cell subclass have been obtained in vitro from rat bone marrow cultured in the presence of conditioned medium from antigen-activated immune mesenteric lymph nodes (13,14). These mast cells stain with alcian blue but not safranin, have low amounts of histamine, contain RMCP-II, and appear to have a non-heparin proteoglycan (15).Thymic-dependent proliferation of mast cells also occurs in the mucosa of helminth-infected mice (16), and mouse hematopoietic stem cells cultured in vitro in the presence of lymphocyte conditioned medium differentiate into mast cells resembling the mucosal mast cell subclass (17-22). The cultured cells depend on the T-cell lymphokine interleukin 3 for growth (23) and contain an oversulfated chondroitin sulfate proteoglycan, termed chondroitin sulfate...

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“…The culture system that was used in this study pro vided a connective tissue microenvironment for mast cell differentiation so that lesser concentrations of lymphoid or bone marrow cells were plated as com pared to other systems which lacked monolayers [10,16,17,20,22,25], The embryonic skin monolayers, which can contain over 1% mast cells in the starting trypsinates, were grown and subcultured at low plat ing densities to avoid monolayer mast cell contamina tion. Using this culture system, we have obtained evi dence that T cells or T cell products can both enhance or suppress the differentiation or proliferation of mast cells.…”

Section: Discussionmentioning

confidence: 99%

“…Based partly on these early in vitro studies [6,8,12] as well as in vivo studies of a T celldependent, Nippostrongylus brasiliensis (Nb)-induced intestinal mastocytosis [15,18], Burnet [3] suggested that there were two mast cell types: one which devel oped from postmitotic T cells accumulating at sites of immune response such as helminth-infected gut and the other represented by the more typical mast cells characteristic of murine connective tissues and serous spaces. However, it became clear from many subsequent in vitro studies that T cells were required not as precursors but as inducers since T cell super natants could fulfill the T cell requirement [10,16,17,20,22,25]. Alternatively, the in vivo work of Kitamura et al [13] suggested that many mast cell types required no T cell influence for differentiation.…”

Section: Introductionmentioning

confidence: 99%

Mast Cell Differentiation in Cultures of T Cell-Depleted Mesenteric Lymph Node Cells from <i>Nippostrongylus brasiliensi</i><i>s</i>-lnfected Mice

Huff

Justus²

1988

Int Arch Allergy Immunol

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Lymphoid and bone marrow cells from normal and horse serum-immunized mice and lymphoid cells from Nippostrongylus brasiliensis-infected mice were cultured on monolayers of embryonic skin fibroblasts to analyze the factors which regulate the differentiation and proliferation of mast cells in vitro. Our results indicate that T cells can regulate the development of mast cells in vitro by either enhancement or suppression. In cultures of horse serum-immune spleen cells, inducer T cells are required for mast cells to develop. However, in cultures of mesenteric lymph node cells from N. brasiliensis-infected mice, inducer T cells are not required for mast cell development. This suggests that the development of mast cells may occur at discrete interleukin-3 (IL-3)-dependent and IL-3-independent stages. Mast cell precursors in the mesenteric lymph nodes of N. brasiliensis-infected mice may have already been acted on by inducer cells in vivo to become mast cell committed. While IL-3 does not appear to be required for mast cell development, these precursors do require the presence of a connective tissue microenvironment such as embryonic skin. The precursors can be inhibited from development by interferon preparations.

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A hemopoietic cell line dependent upon a factor in pokeweed mitogen‐stimulated spleen cell conditioning medium

Cited by 51 publications

References 12 publications

Proliferation of peritoneal mast cells in the skin of W/Wv mice that genetically lack mast cells.

Proliferation of peritoneal mast cells in the skin of W/Wv mice that genetically lack mast cells.

Homology of the rat basophilic leukemia cell and the rat mucosal mast cell.

Mast Cell Differentiation in Cultures of T Cell-Depleted Mesenteric Lymph Node Cells from <i>Nippostrongylus brasiliensi</i><i>s</i>-lnfected Mice

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