A High‐Affinity Fluorescent Sensor for Catecholamine: Application to Monitoring Norepinephrine Exocytosis

Abstract

Af luorescent sensor for catecholamines,N S510, is presented. The sensor is based on aq uinolone fluorophore incorporating ab oronic acid recognition element that gives it high affinity for catecholamines and at urn-on response to norepinephrine.T he sensor results in punctate staining of norepinephrine-enriched chromaffin cells visualizedu sing confocal microscopyi ndicating that it stains the norepinephrine in secretory vesicles.A mperometry in conjunction with total internal reflection fluorescence (TIRF) microscopy demonstrates that the sensor can be used to observe destaining of individual chromaffin granules upon exocytosis.N S510 is the highest affinity fluorescent norepinephrine sensor currently available and can be used for measuring catecholamines in live-cell assays.

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