A High-Capacity Pressurized Continuous Chromatograph

Begovich, J.M.; Byers, Charles H.; Sisson, Warren G.

doi:10.1080/01496398308059923

Cited by 37 publications

(14 citation statements)

References 10 publications

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“…To incorporate the specific character of each process a proper formulation of the corresponding initial and boundary conditions is necessary. Usually adsorbents that are not preloaded are applied leading to the following initial condition: (8) For the two discontinuous processes (elution and recycling) at the column inlet the following holds:…”

Section: Reviewmentioning

confidence: 99%

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Comparison of Various Process Engineering Concepts of Preparative Chromatography

Heuer¹,

Kniep²,

Falk

et al. 1998

Chem. Eng. Technol.

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Liquid chromatography utilizing porous solid phases (adsorption chromatography) is one of the most powerful and versatile methods of modern analytical chemistry. Its widespread application is due in part to the development of a broad range of combinations of solid stationary and liquid mobile phases. Normal phase and reverse phase chromatography with pure and chemically modified silica, respectively, affinity chromatography, can be cited as examples [1]. In addition to its use as an analytical method, chromatography is becoming increasingly important in the preparative scale for the isolation of pure substances in the pharmaceutical industry, in biotechnology, and in the production of fine chemicals. In many cases, the required purity criteria can only be met with chromatographic techniques, as in the resolution of racemates. From a process engineering viewpoint, a better understanding of concentration profile deformations caused by intended overloading of separation columns has recently been acquired [2]. In particular, this concerns the description of displacement and entrainment effects associated with the competitive adsorption. A series of alternative operating modes has been developed and used [3] to enhance yields and productivities. In spite of a number of attempted comparative studies [4], quantitative information about optimizing these processes is lacking. This article will compare four different process engineering concepts of preparative chromatography on the basis of a uniform theoretical model.

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Section: Reviewmentioning

confidence: 99%

“…The SMB process is described, for example, in [3,6,7,13]. The process of annular chromatography uses a stationary phase fixed in the annular space between two concentric cylinders [8]. Across the whole cross section, the mobile phase is transported continuously from top to bottom.…”

Section: Process Engineering Conceptsmentioning

confidence: 99%

Comparison of Various Process Engineering Concepts of Preparative Chromatography

Heuer¹,

Kniep²,

Falk

et al. 1998

Chem. Eng. Technol.

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“…To date, annular chromatography has been applied mostly to isocratic elution chromatography of metals (Begovich and Sisson, 1982;Begovich et al 1983;1 Begovich and Sisson, 1984;Canon and Sisson, 1978;Canon et al, 1980;Carta et al, 1989;Sun et al, 1994), sugars and oligosaccharides (Bart et al, 1996;Byers et al, 1990;Howard et al, 1988;Takahashi and Goto, 1994;Wolfgang et al, 1997), amino acids (DeCarli II et al, 1990;Kitakawa et al, 1995;Kitakawa et al, 1997;Goto, 1991a, 1991b;Yonemoto et al, 1993) and proteins (Bloomingburg et al, 1991, Genest et al, 1998, Takahashi and Goto, 1992. Just a few applications involve the use of (step) gradient elution and fewer still consider the separation of an authentic feed mixture.…”

Section: Introductionmentioning

confidence: 98%

Continuous isolation of plasmid DNA by annular chromatography

Giovannini

Freitag²

2002

Biotech & Bioengineering

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Continuous chromatographic separations, especially of multicomponent mixtures, constitute interesting options for biotechnological downstream processing. Taking the separation of plasmid DNA from clearified lysates on hydroxyapatite as a pertinent example, we discuss the potential of continuous annular chromatography (CAC) in comparison with conventional (preparative) batch chromatography. In CAC the column is realized in the form of a thin (5 mm, height 210 mm) slowly rotating annulus. The performance of such a CAC column is compared to that of an ("analytical") batch column of similar thickness (diameter) and length (4 x 250 mm) and that of a ("preparative") batch column of similar cross-sectional surface area and height (50 x 210 mm). The quality of the obtained plasmid as defined by the appearance of the corresponding agarose gels (native and linearized plasmid), the 260/280 ratio and the biological activity (transient transfection of HEK 293 cells) was found to be identical in all three cases. The yields are also shown to be equivalent. The loading factor is found to be the most decisive parameter for the transfer of a given separation method between the continuous and the batch columns. Under nonoptimized conditions, plate numbers tended to be lower in the continuous compared to the batch columns. This is shown to be largely due to an artifact created by the CAC design (collection of averaged fractions at the outlets) and can be overcome by optimizing the rotation speed. Surprisingly the large batch column consistently gave better plate numbers than either the small batch or the CAC column. Compared to the preparative batch column, wall effects are more pronounced in the CAC (respectively the small diameter batch column), which may translate into better bed stability but conceivably also contributes to an increase in plate height, due to the reduction in bed density usually observed in the proximity of the wall. The CAC is shown to be a powerful approach to continuous chromatography, which allows a direct and straightforward upscale of chromatographic bioseparation methods.

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“…Several more complicated modes of operation were developed in order to overcome the limitations of batch chromatography with respect to productivity, recovery and solvent economy. Examples are displacement chromatography [2], different variants of recycling chromatography [3,4] and annular chromatography [5]. Overviews are given, e.g., in [1,6,7].…”

Section: Introductionmentioning

confidence: 99%

New Developments in Simulated Moving Bed Chromatography

2008

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Chromatographic separation processes are widely used to isolate and purify value added products. Most frequently, such separation processes are performed exploiting the principles of injecting samples of the feed mixture in a repetitive periodic manner and collecting the target products batchwise. In the early 1960s, an alternative operation principle based on using several columns connected in series and exploiting a continuous countercurrent movement between the mobile and stationary phases initiated significant improvements in performing specific separation processes in the petrochemical and sugar industries. In the last decade, the so-called simulated moving bed (SMB) chromatography has rapidly entered the pharmaceutical industry. In particular enantioseparations using chiral stationary phases have become a strong driving force to develop more sophisticated concepts of continuous chromatography. Currently, the growing interest in efficient methods for the downstream processing of biomolecules enhances interest in SMB processes. This paper gives a brief overview of the new suggestions and trends. © 2008 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim [accessed July 4, 2008

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A High-Capacity Pressurized Continuous Chromatograph

Cited by 37 publications

References 10 publications

Comparison of Various Process Engineering Concepts of Preparative Chromatography

Comparison of Various Process Engineering Concepts of Preparative Chromatography

Continuous isolation of plasmid DNA by annular chromatography

New Developments in Simulated Moving Bed Chromatography

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