A High-Density Genetic Linkage Map of SLAFs and QTL Analysis of Grain Size and Weight in Barley (Hordeum vulgare L.)

Abstract

Grain size is an important agronomic trait determines yield in barley, and a high-density genetic map is helpful to accurately detect quantitative trait loci (QTLs) related to grain traits. Using specific-locus amplified fragment sequencing (SLAF-seq) technology, a high-density genetic map was constructed with a population of 134 recombinant inbred lines (RILs) deriving from a cross between Golden Promise (GP) and H602, which contained 12,635 SLAFs with 26,693 SNPs, and spanned 896.74 cM with an average interval of 0.07 cM on seven chromosomes. Based on the map, a total of 16 QTLs for grain length (GL), grain width and thousand-grain weight were detected on 1H, 2H, 4H, 5H, and 6H. Among them, a major QTL locus qGL1, accounting for the max phenotypic variance of 16.7% was located on 1H, which is a new unreported QTL affecting GL. In addition, the other two QTLs, qGL5 and qTGW5, accounting for the max phenotypic variances of 20.7 and 21.1%, respectively, were identified in the same region, and sequencing results showed they are identical to HvDep1 gene. These results indicate that it is a feasible approach to construct a high-quality genetic map for QTL mapping by using SLAF markers, and the detected major QTLs qGL1, qGL5, and qTGW5 are useful for marker-assisted selection (MAS) of grain size in barley breeding.

Stable pleiotropic loci and candidate genes for fresh pod‐ and seed‐related characteristics across multiple environments in soybean (Glycine max)

“…In all, 10 continuously arranged pods were treated as one group to measure the fresh pod length and width, and then the weights of pods and seeds were measured. After that, the soybean seeds were scanned and the seed length, seed width and seed area were analysed through the SC‐G automatic seed testing system (Hangzhou WSeen Detection Technology Co., Ltd.; Fang et al., 2020; Lei et al., 2020).…”

Advances in Barley Breeding for Improving Nitrogen Use Efficiency

“…The constraints of bi-parental mapping population construction have also been greatly reduced because of the high density of SNP markers. There have been some reports of this kind of work on barley, such as studies involving plant height [47], net blotch disease [48], yield and grain plumpness [49], grain amylopectin content [50], and grain size and weight [51]. However, there are no reports on NUE.…”

Mining of quantitative trait loci and candidate genes for seed size and shape across multiple environments in soybean (Glycine max)

“…Six related traits of ZQ-RIL for soybean seed size and shape, including the seed length (SL), seed width (SW), seed diameter (SD), seed circumference (SC), ratio of seed length to width (RSLW), and hundredseed weight (HSW), were analysed under four environments via the methods similar as in the previous studies (Fang et al, 2020;Lei et al, 2020). Briefly, 100 soybean seeds of ZQ-RIL population lines were scanned and analysed by the SC-G automatic seed testing system (Hangzhou WSeen Detection Technology Co., Ltd., China), and the six related traits were calculated as single seed values except for the hundred-seed weight.…”