A high-density, integrated genetic linkage map of lettuce (Lactuca spp.)

Truco, María José; Antonise, Rudie; Lavelle, Dean; Ochoa, O.; Kozik, Alexander; Witsenboer, H.; Fort, Sean B.; Jeuken, Marieke; Kesseli, Rick; Lindhout, P.; Michelmore, Richard W.; Peleman, J.

doi:10.1007/s00122-007-0599-9

Cited by 104 publications

(104 citation statements)

References 47 publications

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“…We identified one (cross 1) and seven (cross 2) viable 9sal plants (see Supplemental Table 1A online, right column). None of these eight plants were homozygous for L. sativa alleles in a region from ;30 to 35 cM on chromosome 6 (hereafter referred to as the C6 locus, and similar to C9, genotypes on chromosome 6 are referred to as 6sal, 6sat, and 6het; see Supplemental Table 1A online; equivalent to chromosome 8 of the consensus map for Lactuca spp; Truco et al, 2007). Thus, a homozygous combination of L. sativa alleles on the C6 locus and L. saligna alleles on the C9 locus (6sat9sal) was implicated as causing necrosis leading to lethality.…”

Section: Two Epistatic Loci Are Involved In Hybrid Necrosismentioning

confidence: 99%

Rin4 Causes Hybrid Necrosis and Race-Specific Resistance in an Interspecific Lettuce Hybrid

Jeuken¹,

Zhang²,

et al. 2009

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Some inter-and intraspecific crosses may result in reduced viability or sterility in the offspring, often due to genetic incompatibilities resulting from interactions between two or more loci. Hybrid necrosis is a postzygotic genetic incompatibility that is phenotypically manifested as necrotic lesions on the plant. We observed hybrid necrosis in interspecific lettuce (Lactuca sativa and Lactuca saligna) hybrids that correlated with resistance to downy mildew. Segregation analysis revealed a specific allelic combination at two interacting loci to be responsible. The allelic interaction had two consequences: (1) a quantitative temperature-dependent autoimmunity reaction leading to necrotic lesions, lethality, and quantitative resistance to an otherwise virulent race of Bremia lactucae; and (2) a qualitative temperature-independent racespecific resistance to an avirulent race of B. lactucae. We demonstrated by transient expression and silencing experiments that one of the two interacting genes was Rin4. In Arabidopsis thaliana, RIN4 is known to interact with multiple R gene products, and their interactions result in hypersensitive resistance to Pseudomonas syringae. Site-directed mutation studies on the necrosis-eliciting allele of Rin4 in lettuce showed that three residues were critical for hybrid necrosis.

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Section: Two Epistatic Loci Are Involved In Hybrid Necrosismentioning

confidence: 99%

Rin4 Causes Hybrid Necrosis and Race-Specific Resistance in an Interspecific Lettuce Hybrid

Jeuken¹,

Zhang²,

et al. 2009

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“…Integrated consensus maps have been constructed for many cultivated plant species like loblolly pine (Sewell et al 1999), soybean (Cregan et al 1999;Song et al 2004), rapeseed (Lombard and Delourme 2001), wheat (Daryl et al 2004), grapevine (Doligez et al 2006), and lettuce (Truco et al 2007). Composite maps derived from multiple populations provide several advantages.…”

Section: Introductionmentioning

confidence: 99%

Construction of an integrated consensus map of the apple genome based on four mapping populations

N’Diaye

Weg

Kodde

et al. 2008

Tree Genetics & Genomes

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An integrated consensus genetic map for apple was constructed on the basis of segregation data from four genetically connected crosses (C1=Discovery × TN10-8, C2=Fiesta × Discovery, C3=Discovery × Prima, C4= Durello di Forli × Fiesta) with a total of 676 individuals using CarthaGene® software. First, integrated female-male maps were built for each population using common femalemale simple sequence repeat markers (SSRs). Then, common SSRs over populations were used for the consensus map integration. The integrated consensus map consists of 1,046 markers, of which 159 are SSR markers, distributed over 17 linkage groups reflecting the basic chromosome number of apple. The total length of the integrated consensus map was 1,032 cM with a mean distance between adjacent loci of 1.1 cM. Markers were proportionally distributed over the 17 linkage groups (χ 2 = 16.53, df=16, p=0.41). A non-uniform marker distribution was observed within all of the linkage groups (LGs). Clustering of markers at the same position (within a 1-cM window) was observed throughout LGs and consisted predominantly of only two to three linked markers. The four integrated female-male maps showed a very good colinearity in marker order for their common markers, except for only two (CH01h01, CH05g03) and three (CH05a02z, NZ02b01, Lap-1) markers on LG17 and LG15, respectively. This integrated consensus map provides a framework for performing quantitative trait locus (QTL) detection in a multi-population design and evaluating the genetic background effect on QTL expression.

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“…Compared with other crops such as rice and soybean [48], there are relatively few public SSR markers available in Brassica, although several research groups have presented a number of SSR markers with different technology [16,34,35,37,49]. SSR development has been improved since the early expensive technology of prob hybridization to an easy online source-based method.…”

Section: Discussionmentioning

confidence: 99%

An Integrated Genetic Map for Brassica napus Derived from Double Haploid and Recombinant Inbred Populations

Geng

Javed²,

McVetty³

et al. 2012

Hereditary Genetics

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A hybrid developed from a cross between two diverse Brassica napus cultivars ("Polo" and "Topas") was used to produce a microspore derived double haploid (DH) population and a single seed descent derived recombinant inbred (RI) population for genetic mapping. Each of the two populations consisting of 190 DH lines and 94 RI lines was characterized for various types (SSR, SRAP, ISSR, SCAR) of polymorphic molecular markers. The DH population was scored for 620 molecular markers while the RI population was scored for 349 molecular markers to construct two independent genetic maps. In both genetic maps, all of the molecular markers were found to cluster in 19 linkage groups (LGs) covered a total genome length of 2244.1 cM and 1649.1 cM for the DH and RI maps, respectively. The data from the two genetic maps was used to construct a consensus integrated genetic map covering a total genome length of 2464.9 cM. Previously published Brassica reference genetic maps were used to assign each of the nineteenLGs to corresponding Brassica napus chromosomes named N01 to N19. To our knowledge, this is the first integrated genetic map based on DH and RI populations developed from the same cross in Brassica napus.

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A high-density, integrated genetic linkage map of lettuce (Lactuca spp.)

Cited by 104 publications

References 47 publications

Rin4 Causes Hybrid Necrosis and Race-Specific Resistance in an Interspecific Lettuce Hybrid

Rin4 Causes Hybrid Necrosis and Race-Specific Resistance in an Interspecific Lettuce Hybrid

Construction of an integrated consensus map of the apple genome based on four mapping populations

An Integrated Genetic Map for Brassica napus Derived from Double Haploid and Recombinant Inbred Populations

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