A High Performance Liquid Chromatographic Method for Histamine in Plasma Using Solid Phase Extraction and Fluorescamine Derivatization

Highly Sensitive Determination of Histamine by Narrow-Bore High-Performance Liquid Chromatography Using Postcolumn Fluorescence Detection

“…Highly specific and sensitive highperformance liquid chromatography (HPLC) methods have also been reported. [5][6][7][8][9][10][11][12][13][14][15][16][17][18] Widely used today is postcolumn fluorescence detection; 5,7 this employs a twostep condensation reaction with o-phthalaldehyde (OPA) under alkaline conditions, followed by acidic conditions. 19 Although the specificity of this method for histamine is high, the sensitivity and reproducibility are still problematic in terms of reagent transfer and OPA stability in aqueous solutions.…”