A High-performance Liquid-chromatographic Microanalytical Procedure for the Rapid Estimation of Piracetam in Plasma or Cerebrospinal Fluid

Doheny, Mary H.; O’Connell, Mark; Patsalos, Philip N.

doi:10.1111/j.2042-7158.1996.tb05964.x

Cited by 12 publications

(2 citation statements)

References 11 publications

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“…, 1990) and liquid chromatography with ultraviolet detection (LC‐UV) (Curticapean and Imre, 2007; Doheny et al. , 1996; Louchahi et al. , 1995; Mascher and Kikuta, 1989; Rameis et al.…”

Section: Introductionmentioning

confidence: 99%

Determination of piracetam in rat plasma by LC–MS/MS and its application to pharmacokinetics

Wang¹,

Zhu²,

Xu³

et al. 2010

Biomedical Chromatography

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A sensitive and selective liquid chromatography-tandem mass spectrometry method for the determination of piracetam in rat plasma was developed and validated over the concentration range of 0.1-20 mg/mL. After addition of oxiracetam as internal standard, a simplified protein precipitation with trichloroacetic acid (5%) was employed for the sample preparation. Chromatographic separation was performed by a Zorbax SB-Aq column (150 ¥ 2.1 mm, 3.5 mm). The mobile phase was acetonitrile-1% formic acid in water (10:90 v/v) delivered at a flow rate of 0.3 mL/min. The MS data acquisition was accomplished in multiple reaction monitoring mode with a positive electrospray ionization interface. The lower limit of quantification was 0.1 mg/mL. For inter-day and intra-day tests, the precision (RSD) for the entire validation was less than 9%, and the accuracy was within the 94.6-103.2% range. The developed method was successfully applied to pharmacokinetic studies of piracetam in rats following single oral administration dose of 50 mg/kg.

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“…, 1990) and liquid chromatography with ultraviolet detection (LC‐UV) (Curticapean and Imre, 2007; Doheny et al. , 1996; Louchahi et al. , 1995; Mascher and Kikuta, 1989; Rameis et al.…”

Section: Introductionmentioning

confidence: 99%

Determination of piracetam in rat plasma by LC–MS/MS and its application to pharmacokinetics

Wang¹,

Zhu²,

Xu³

et al. 2010

Biomedical Chromatography

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“…The extracts were analyzed by HPLC-MS/MS [94]. The HPLC methods describe using a mixture of an aqueous solution of perchloric acid, methanol or acetonitrile with protein precipitation and LLE with chloroform, 2-propanol, toluene or hexane mixture as a form of simple extraction [95,96].…”

Section: Piracetammentioning

confidence: 99%

New Methods Used in Pharmacokinetics and Therapeutic Monitoring of the First and Newer Generations of Antiepileptic Drugs (AEDs)

et al. 2020

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The review presents data from the last few years on bioanalytical methods used in therapeutic drug monitoring (TDM) of the 1st–3rd generation and the newest antiepileptic drug (AEDs) cenobamate in patients with various forms of seizures. Chemical classification, structure, mechanism of action, pharmacokinetic data and therapeutic ranges for total and free fractions and interactions were collected. The primary data on bioanalytical methods for AEDs determination included biological matrices, sample preparation, dried blood spot (DBS) analysis, column resolution, detection method, validation parameters, and clinical utility. In conclusion, the most frequently described method used in AED analysis is the LC-based technique (HPLC, UHPLC, USLC) combined with highly sensitive mass detection or fluorescence detection. However, less sensitive UV is also used. Capillary electrophoresis and gas chromatography have been rarely applied. Besides the precipitation of proteins or LLE, an automatic SPE is often a sample preparation method. Derivatization was also indicated to improve sensitivity and automate the analysis. The usefulness of the methods for TDM was also highlighted.

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Comparison of piracetam measured with HPLC-DAD, HPLC-ESI-MS, DIP-APCI-MS, and a newly developed and optimized DIP-ESI-MS

Lenzen

Winterfeld²,

Schmitz

2016

Anal Bioanal Chem

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The direct inlet probe-electrospray ionization (DIP-ESI) presented here was based on the direct inlet probe-atmospheric pressure chemical ionization (DIP-APCI) developed by our group. It was coupled to an ion trap mass spectrometer (MS) for the detection of more polar compounds such as degradation products from pharmaceuticals. First, the position of the ESI tip, the gas and solvent flow rates, as well as the gas temperature were optimized with the help of the statistic program Minitab® 17 and a caffeine standard. The ability to perform quantitative analyses was also tested by using different concentrations of caffeine and camphor. Calibration curves with a quadratic calibration regression of R (2) = 0.9997 and 0.9998 for caffeine and camphor, respectively, were obtained. The limit of detection of 2.5 and 1.7 ng per injection for caffeine and camphor were determined, respectively. Furthermore, a solution of piracetam was used to compare established analytical methods for this drug and its impurities such as HPLC-diode array detector (DAD) and HPLC-ESI-MS with the DIP-APCI and the developed DIP-ESI. With HPLC-DAD and 10 μg piracetam on column, no impurity could be detected. With HPLC-ESI-MS, two impurities (A and B) were identified with only 4.6 μg piracetam on column, while with DIP-ESI, an amount of 1.6 μg piracetam was sufficient. In the case of the DIP-ESI measurements, all detected impurities could be identified by MS/MS studies. Graphical Abstract Scheme of the DIP-ESI principle.

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A High-performance Liquid-chromatographic Microanalytical Procedure for the Rapid Estimation of Piracetam in Plasma or Cerebrospinal Fluid

Cited by 12 publications

References 11 publications

Determination of piracetam in rat plasma by LC–MS/MS and its application to pharmacokinetics

Determination of piracetam in rat plasma by LC–MS/MS and its application to pharmacokinetics

New Methods Used in Pharmacokinetics and Therapeutic Monitoring of the First and Newer Generations of Antiepileptic Drugs (AEDs)

Comparison of piracetam measured with HPLC-DAD, HPLC-ESI-MS, DIP-APCI-MS, and a newly developed and optimized DIP-ESI-MS

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