A high performance liquid chromatography method for determining nitrate and nitrite levels in vegetables

Chou, Shin-Shou; Chung, J.-C.; Hwang, Deng Fwu

doi:10.38212/2224-6614.2702

Cited by 30 publications

(37 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…e HPLC method used in this work was previously used to determine the nitrate content in canned vegetable juices [28], vegetables [29], and sodium nitrate mouthwash [30,31]. According to this work, the HPLC method was also applied to determine the nitrate content in C. anisata leaves.…”

Section: Resultsmentioning

confidence: 99%

“…is method showed a linear response and specific, precise, and accurate style. Moreover, this method was easy and rapid [28,29,32]. e advantages of this method were the high stability and efficiency of the columns and the lack of the organic stationary phase to be regenerated [32].…”

Section: Discussionmentioning

confidence: 99%

“…e HPLC used for the analysis of the nitrate content was adapted from Cheng and Tsang [28] and Chou et al [29].…”

Section: Analysis Of Nitrate Content and Methods Validationmentioning

confidence: 99%

See 2 more Smart Citations

Validated Reversed-Phase Ion-Interaction High-Performance Liquid Chromatography for Quantitation of Nitrate Content ofClausena anisata(Willd.) Hook. f. ex Benth. Leaves

2020

View full text Add to dashboard Cite

This work sought to validate the reversed-phase ion-interaction high-performance liquid chromatography for quantifying the nitrate content in the extract and raw material of Clausena anisata (Willd.) Hook. f. ex Benth. leaves. Three extraction methods (i.e., decoction, infusion, and ultrasound-assisted extraction) were investigated and compared. Furthermore, the effect of the solid-to-solvent ratio and defatting was also evaluated. The validation result showed that the high-performance liquid chromatographic method had a linear response (R2 = 0.9999) in the range of 1–50 μg/mL. The limit of detection and limit of quantitation were 0.25 μg/mL and 0.75 μg/mL, respectively. In addition, the method was specific, precise, and accurate. So the validated method was suitable for determination of the nitrate content in C. anisata leaves. Infusion of a nondefatted sample using a solid-to-solvent ratio of 1 : 10 gave the highest nitrate content in the raw material, 0.251 ± 0.003%. In case of a defatted sample, decoction provided the highest nitrate content, 0.309 ± 0.001%. Increasing the solid-to-solvent ratio and defatting had a huge effect on the nitrate content of C. anisata leaves extracted from decoction. To the best of our knowledge, this is the first report that used the reversed-phase ion-interaction high-performance liquid chromatography for quantifying the nitrate content in C. anisata leaves. Furthermore, the authors suggested that nitrate could be used as a standard marker for quality control of C. anisata leaves’ extract and raw material.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Validated Reversed-Phase Ion-Interaction High-Performance Liquid Chromatography for Quantitation of Nitrate Content ofClausena anisata(Willd.) Hook. f. ex Benth. Leaves

2020

View full text Add to dashboard Cite

show abstract

“…[4]. Vegetables are the most important source of nitrate exposure in the human diet and contribute to the intake of more than 80% of nitrates [1,5]. Excessive usage of nitrogenous fertilizers has increased the nitrate concentration in various vegetables.…”

Section: Introductionmentioning

confidence: 99%

The nitrate content of fresh and cooked vegetables and their health-related risks

et al. 2020

View full text Add to dashboard Cite

Background Vegetables are the most important source of nitrates in the human diet. During various processes in the body, nitrates are converted into nitrites, which causes various diseases, such as blue baby syndrome and cancer. This study aimed to determine the concentration of nitrates in several vegetable farms in Sanandaj city and to evaluate their health-related risks. Methods This descriptive cross-sectional study was conducted from October 2017 to July 2018. A total of 90 samples were taken from nine farms. Soil and water sampling was also carried out. All stages of sample preparation and extraction were carried out according to Food Standards 2-16721, and the nitrate measurements were performed using ion chromatography (Compact IC Plus 882 Model, Metrohm, Switzerland). A health risk assessment was performed using the non-carcinogenic risk assessment. Results This study's results showed that the nitrate concertation in all vegetables was less than National Iranian Vegetable Nitrate Standard. Nitrate levels in leafy vegetables were higher than in root vegetables, and the root vegetables levels were higher than those in Fruit vegetable. The nitrate level in vegetables in autumn was higher than in spring. The cooking process reduced the raw vegetables' nitrate content from 4.094% to 13.407%, while the frying process increased the vegetables' nitrate content from 12.46% to 29.93%. The highest health risk level in raw, cooked and fried vegetables was parsley, parsley and beet leaves, respectively, and the lowest in all categories was tomatoes. Generally, the highest health risk was related to fried beet leaves, and the lowest was raw tomatoes. In addition, each of the abovementioned relationships between vegetables' nitrate levels and the harvest season, type of processing procedure and type of vegetables was significant (p < 0.05). The

show abstract

“…During the past decade, IC and HPLC methods have been developed for analysis of nitrite and nitrate, due to by their accurate and precise determinations of the compounds [4,10,12,13].…”

Section: Introductionmentioning

confidence: 99%

"Determination of nitrate and nitrite content in Zonar milk serum and derived dairy drinks using ion-pair reversed-phase high performance liquid chromatography "

Filip¹,

Prodan²,

Moldovan³

et al. 2019

Studia UBB Chemia

View full text Add to dashboard Cite

The presence of nitrite and nitrate in milk and dairy products may cause serious health problem for consumers. Thus, a simple and accurate ion-pair reversed-phase high performance liquid chromatographic (IP-RP-HPLC) method has been developed and validated for determination of nitrite and nitrate in some dairy drinks based on Zonar Milk Serum. The separation was achieved on a Synergy Hydro-RP polar endcapped C18, 80Å, 4m (250  4.6 mm) column using isocratic elution with 0.01M n-octylamine and methanol, 90:10 (v/v) at pH=7; with flow rate of 1 mL•min-1 and detection at 214 nm and 25°C. A linear response (R 2 ≥ 0.9994) was observed in the range between 3.125 and 75 µg mL-1 for the nitrite and nitrate standard solutions. The limits of detection (LOD) and quantification (LOQ) were found as 0.25 µg mL-1 and 0.8 µg mL-1 for nitrite and 0.4 µg mL-1 and 1.2 µg mL-1 for nitrate, respectively. The method was validated according to ICH guidelines with respect to selectivity, linearity, detection and quantitation limits, accuracy, precision. The good analytical performance verified for this method indicates that it is suitable the monitoring of nitrite and nitrate in dairy drinks based on Zonar Milk Serum.

show abstract

A high performance liquid chromatography method for determining nitrate and nitrite levels in vegetables

Cited by 30 publications

References 12 publications

Validated Reversed-Phase Ion-Interaction High-Performance Liquid Chromatography for Quantitation of Nitrate Content ofClausena anisata(Willd.) Hook. f. ex Benth. Leaves

Validated Reversed-Phase Ion-Interaction High-Performance Liquid Chromatography for Quantitation of Nitrate Content ofClausena anisata(Willd.) Hook. f. ex Benth. Leaves

The nitrate content of fresh and cooked vegetables and their health-related risks

"Determination of nitrate and nitrite content in Zonar milk serum and derived dairy drinks using ion-pair reversed-phase high performance liquid chromatography "

Contact Info

Product

Resources

About