A high proportion of Baltic Sea benthic cyanobacterial isolates contain apoptogens able to induce rapid death of isolated rat hepatocytes

Herfindal, Lars; Oftedal, Linn; Selheim, Frode; Wahlsten, Matti; Sivonen, Kaarina; Døskeland, Stein Ove

doi:10.1016/j.toxicon.2005.04.005

Cited by 29 publications

(56 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition, we analyzed two microcystin-producing Anabaena strains, which had previously been isolated from the coastal waters of Helsinki City, Finland ( Fig. 1) (14). Anabaena strain 315 was isolated in August 1997 from a plankton sample taken at Seurasaari in Helsinki.…”

Section: Methodsmentioning

confidence: 99%

Direct Evidence for Production of Microcystins by Anabaena Strains from the Baltic Sea

Halinen

Jokela

Fewer

et al. 2007

Appl Environ Microbiol

Self Cite

View full text Add to dashboard Cite

show abstract

Section: Methodsmentioning

confidence: 99%

Direct Evidence for Production of Microcystins by Anabaena Strains from the Baltic Sea

Halinen

Jokela

Fewer

et al. 2007

Appl Environ Microbiol

Self Cite

View full text Add to dashboard Cite

show abstract

“…This potent cyclic peptide is an inhibitor of microcystin-induced apoptosis in hepatocytes, an activity not previously associated with cyanobacterial natural compounds. Herfindal et al (2005) and Surakka et al (2005) also showed that benthic strains of Anabaena, Phormidium and Nostoc genera could cause cytotoxicity and apoptosis in mammalian cell lines by uncommon compounds, again not yet characterized. In addition, have shown cytotoxicity against L929 cells and antiviral activity by estuarine cyanobacteria.…”

Section: Further Cyanotoxinsmentioning

confidence: 99%

“…Nevertheless, recently, benthic cyanobacteria species of the genera Anabaena, Calothrix, Cyanothece, Nostoc, Oscillatoria and Phormidium, have been shown to produce toxins such as MCs, anatoxin and BMAA, and other cytotoxic and apoptosis-inducing compounds in several European estuarine systems, such as in France, Norway, Finland, Switzerland, Poland, Scotland and Portugal (Edwards et al, 1992;Mez et al, 1997;Vasconcelos & Cerqueira, 2001;Aboal & Puig, 2005;Herfindal et al, 2005;Surakka et al, 2005;Jonasson et al, 2008;Oftedal et al, 2010). Furthermore, the presence of anabaenopeptins A and D in a benthic brackish-water Nostoc isolate from an Atlantic estuary (Minho River, Portugal) has been reported by Lopes and co-workers (unpublished information).…”

Section: Further Cyanotoxinsmentioning

confidence: 99%

“…E-mail: vmvascon@ fc.up.pt Tan, 2007). The cyanobacteria of brackish waters (including estuaries), however, remain under-investigated, particularly in Europe, despite a number of significant contributions (Sivonen et al, 1989;Nehring, 1993;Lehtima¨ki et al, 1994Lehtima¨ki et al, , 1997Lehtima¨ki et al, , 2000Wille´n & Mattson, 1997;Sivonen & Jones, 1999;Sipia¨et al, 2001Sipia¨et al, , 2002Sipia¨et al, , 2006Sipia¨et al, , 2007Sipia¨et al, , 2008Vasconcelos & Cerqueira, 2001;Laamanen et al, 2002;Rocha et al, 2002;Geiss et al, 2003;Hobson & Fallowfield, 2003;Lehtonen et al, 2003;Stal et al, 2003;Sobrino et al, 2004;Herfindal et al, 2005;Kankaanpa¨a¨et al, 2005;Karjalainen et al, 2005Karjalainen et al, , 2007Karlsson et al, 2005;Pere´z & Carrilo, 2005;Surakka et al, 2005;Marino et al, 2006;Mazur-Marzec et al, 2007Paoli et al, 2007;Halinen et al, 2008;Ibelings & Havens, 2008;Jo´z´wiak et al, 2008;Lopes et al, 2010;Oftedal et al, 2010;). This lack of information for brackish-water cyanobacteria is especially significant in relation to the potential for production of new bioactive compounds.…”

Section: Introductionmentioning

confidence: 99%

“…The occurrence of Phormidium, Nostoc, Cyanothece and Calothrix in Baltic waters has been reported (Herfindal et al, 2005;Surakka et al, 2005). Interestingly, although the filamentous Prochlorothrix is known to be a genus sensitive to high salinity, the uncommon species P. hollandica has been found in the Baltic Sea, in an environment with salinity up to 12 psu (BurgerWiersma et al, 1989;Geiss et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Planktonic and benthic cyanobacteria of European brackish waters: a perspective on estuaries and brackish seas

Lopes

Vasconcelos

2011

European Journal of Phycology

View full text Add to dashboard Cite

Cyanobacteria are photosynthetic organisms found in aquatic and terrestrial biotopes and ecosystems throughout the world. Marine and freshwater cyanobacteria have been extensively studied due to the toxic hazards that some of them create and biotechnological interest. In contrast, the cyanobacteria of brackish waters have been less studied despite being able to form toxic blooms like their freshwater and marine counterparts. We review the occurrence, diversity and toxicity of cyanobacteria species in the brackish waters of Europe, mainly estuaries and brackish environments of the Atlantic Ocean and the Mediterranean and Baltic Seas. The dominant cyanobacteria belong mainly to the planktonic genera Nodularia, Aphanizomenon, Microcystis and Anabaena, but also the benthic forms of Anabaena and Phormidium. Most genera from brackish waters are reported to be hepatotoxin producers (microcystin and nodularin). However, anatoxin-a and other bioactive compounds (e.g. apoptogens) can also be found and are produced exclusively by benthic forms. Nodularin is the best-characterized brackish-water cyanotoxin, being primarily produced by N. spumigena. Data are presented on cyanotoxin production, accumulation, and potential food chain transfer, with a particular focus on nodularin, which induces multiple effects on food-chain dynamics. Microcystins and anatoxin-a are also considered. Potential risks to animals and humans from cyanotoxin exposure are discussed, although reports of animal poisoning by brackish-water toxic cyanobacteria are scarce and no cases of human intoxication are yet known.

show abstract

A Novel Cyanobacterial Nostocyclopeptide is a Potent Antitoxin against Microcystins

et al. 2010

Self Cite

View full text Add to dashboard Cite

Cyanobacterial hepatotoxins (microcystins and nodularins) cause numerous animal poisonings worldwide each year and are threats to human health. However, we found that extracts from several cyanobacteria isolates failed to induce hepatotoxicity even if they contained high concentrations of the liver toxin microcystin. The antitoxic activity abolishes all morphological hallmarks of microcystin-induced apoptosis, and therefore invalidates cell-based assays of the microcystin content of bloom-forming cyanobacteria. The antitoxin was purified from a cyanobacterial isolate (Nostoc sp. XSPORK 13A) from the Baltic Sea, and the activity was shown to reside in a novel cyclic peptide of the nostocyclopeptide family (nostocyclopeptide M1, Ncp-M1) that consists of seven amino acids (Tyr1-Tyr2-D-HSe3-L-Pro4-L-Val5-(2S,4S)-4-MPr6-Tyr7; MW=881) with an imino linkage between Tyr1 and Tyr7. Ncp-M1 did not compete with labelled microcystin for binding to protein phosphatase 2A; this explains why the antitoxin did not interfere with phosphatase-based microcystin assays. Currently used agents that interfere with microcystin action, such as inhibitors of ROS formation, microcystin uptake and Cam-kinase activity, are themselves inherently toxic. Since Ncp-M1 is potent and nontoxic it promises to become a useful mechanistic tool as soon as its exact cellular target is elucidated.

show abstract

A high proportion of Baltic Sea benthic cyanobacterial isolates contain apoptogens able to induce rapid death of isolated rat hepatocytes

Cited by 29 publications

References 25 publications

Direct Evidence for Production of Microcystins by Anabaena Strains from the Baltic Sea

Direct Evidence for Production of Microcystins by Anabaena Strains from the Baltic Sea

Planktonic and benthic cyanobacteria of European brackish waters: a perspective on estuaries and brackish seas

A Novel Cyanobacterial Nostocyclopeptide is a Potent Antitoxin against Microcystins

Contact Info

Product

Resources

About