A high-quality genome assembly and annotation of the European earwig Forficula auricularia

Abstract

The European earwig Forficula auricularia is an important model for studies of maternal care, sexual selection, sociality and host-parasite interactions. However, detailed genetic investigations of this species are hindered by a lack of genomic resources. Here we present a high-quality hybrid genome assembly for F. auricularia. The genome was assembled using nanopore long-reads and 10x chromium link-reads. The final assembly is 1.06Gb in length with 31.03% GC content. It consists of 919 scaffolds with an N50 of 12.55Mb. Half of the genome is present in only 20 scaffolds. Benchmarking Universal Single-Copy Orthologs scores are ∼90% from three sets of single-copy orthologs (eukaryotic, insect, and arthropod). The total repeat elements in the genome are 64.62%. The MAKER2 pipeline annotated 12,876 protein-coding genes and 21,031 mRNAs. The genome assembly, annotation, and associated resources will be of high value to a large and diverse group of researchers working on Dermapterans.

Whole genome assembly and annotation of the lucerne weevilSitona discoideus

“…We performed flow cytometry analysis using a single head of S. discoideus with two biological replicates at Flowjoanna (Palmerston North, NZ), following the standard procedures described in (Bhattarai, Katuwal et al 2022). Rooster red blood cells (RRBC) obtained from a domestic rooster were used as a reference sample.…”