A high sensitive platinum-modified colloidal gold immunoassay for tenuazonic acid detection based on monoclonal IgG

Cai, Peiyuan; Wang, Rongzhi; Ling, Sumei; Wang, Shihua

doi:10.1016/j.foodchem.2021.130021

Cited by 18 publications

(27 citation statements)

References 37 publications

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“…The successfully prepared AuNPs, with an average diameter of 15 nm, were used as the signal reporters of the AuNPs-based strip, for rapidly detecting αB-CTX. It is well known that the size of the AuNPs is an important factor to improve the sensitivity of detection [ 23 ]. Nanoflower gold particles (AuNFs), with a multi-branched surface and large size, have been widely used as signal reporters to enhance the sensitivity of detection [ 17 ].…”

Section: Resultsmentioning

confidence: 99%

“…The AuNFs were prepared by using the seeding growth method, and the pH of the solution significantly affected the color of the solution and the shape of the AuNFs. A pH of 7.0 in the reaction solution was suitable for the synthesis of AuNFs, according to previous reports [ 17 , 23 ]. As shown in Figure 5 A, the color of the nanoflower solution was deep blue, with good transparency and stability, and the prepared AuNFs showed a complex three-dimensional and flower-like shape under TEM observation.…”

Section: Resultsmentioning

confidence: 99%

“…AuNPs–antibody conjugation was performed as described, with minor modifications [ 23 ]. The prepared colloidal gold solution (about 200 μL) was added into the series of 96-well plates separately, and different volumes of purified mAb solution (from 0 to 7 μL) were added into the colloidal gold solution.…”

Section: Methodsmentioning

confidence: 99%

“…To construct the AuNFs-based strip, the AuNFs probe was prepared according to the reported studies [ 23 ], and all the steps of preparation were similar to those of the AuNPs–antibody conjugates described in the above section. The specificity and sensitivity of the AuNFs-based strip were characterized using the same protocols as the colloid gold strip.…”

Section: Methodsmentioning

confidence: 99%

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Development of Immunochromatographic Strip for Detection of αB-VxXXIVA-Conotoxin Based on 5E4 Monoclonal Antibody

Tang

Liu

Chen

et al. 2022

Toxins

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Given the application of αB-VxXXIVA-conotoxin (αB-CTX) in analgesics and cancer chemotherapeutics, and its threat to humans, it is urgent to develop a rapid, effective and accurate method for the analysis and detection of αB-CTX in real shellfish and medicine drug samples. In the present study, two different immunochromatographic strips were established for αB-CTX detection, based on the monoclonal antibody 5E4 against αB-CTX, and the visual limits of detection (vLOD) for the colloidal gold nanoparticles-based strip (AuNPs-based strip) and nanoflowers-based strip (AuNFs-based strip) were 4 μg/mL and 1.5 μg/mL, respectively. The developed AuNPs-/AuNFs-based strips have good specificity and accuracy, and the detection results were analyzed in less than 10 min, without using an instrument. In view of the excellent repeatability and usability, the established methods could be applied to detect and analyze the content of αB-CTX in real samples.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Development of Immunochromatographic Strip for Detection of αB-VxXXIVA-Conotoxin Based on 5E4 Monoclonal Antibody

Tang

Liu

Chen

et al. 2022

Toxins

Self Cite

View full text Add to dashboard Cite

show abstract

“…Moreover, complicated and tedious operation steps, high requirement of samples and detection practicability seriously limits their application in the detection of αB-CTX in real samples [ 19 ]. In contrast, immunoassays with high sensitivity, accuracy and adaptability have been widely used to detect the targets (toxins, pathogenic molecules and heavy metals) in different samples [ 20 , 21 , 22 ]. In the previous study, we developed ELISA and colloidal test strips based on monoclonal antibodies (mAb) against ω-CTX to detect ω-CTX MVIIA residue in Conus samples [ 5 ].…”

Section: Introductionmentioning

confidence: 99%

Detection of αB-Conotoxin VxXXIVA (αB-CTX) by ic-ELISA Based on an Epitope-Specific Monoclonal Antibody

Tang

Liu

Gao

et al. 2022

Toxins

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In view of the toxicological hazard and important applications in analgesics and cancer chemotherapeutics of αB-CTX, it is urgent to develop an accurate, effective and feasible immunoassay for the determination and analysis of αB-CTX in real samples. In this study, MBP-αB-CTX4 tandem fusion protein was used as an immunogen to elicit a strong immune response, and a hybridoma cell 5E4 secreting IgG2b against αB-CTX was successfully screened by hybridoma technology. The affinity of the purified 5E4 monoclonal antibody (mAb) was 1.02 × 108 L/mol, which showed high affinity and specificity to αB-CTX. Epitope 1 of αB-CTX is the major binding region for 5E4 mAb recongnization, and two amino acid residues (14L and 15F) in αB-CTX were critical sites for the interaction between αB-CTX and 5E4 mAb. Indirect competitive ELISA (ic-ELISA) based on 5E4 mAb was developed to detect and analyze αB-CTX in real samples, and the linear range of ic-ELISA to αB-CTX was 117–3798 ng/mL, with a limit of detection (LOD) of 81 ng/mL. All the above results indicated that the developed ic-ELISA had high accuracy and repeatability, and it could be applied for αB-CTX detection and drug analysis in real samples.

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Advances in nanoprobes‐based immunoassays

Zhang,

Huang,

Jin

et al. 2023

BMEMat

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Immunoassay is a powerful technique that uses highly specific antigen‐antibody interactions to detect biochemical targets such as proteins and toxins. As a diagnostic tool, immunoassay is employed in the screening, diagnosis, and prognosis of diseases, which are crucial for the grasp and control of patient conditions in clinical practice. With the rapid development of nanotechnology, immunoassays based on nanoprobes have attracted more and more attention due to the advantages of high sensitivity, specificity, stability, and versatility. These nanoprobes are nanoscale particles that can act as signal carriers or targeting agents for immunoassays. In this paper, we review the recent advances in various types of nanoprobes for immunoassays, such as colloidal gold, quantum dots, magnetic nanoparticles, nanozymes, aggregation‐induced emission, and up‐conversion nanoparticles. The effect of the nanoprobe construction and synthesis methods on their detection performance deserves to be studied in depth. We also compare their detection ranges and limits in different immunoassay methods, such as lateral flow immunoassays, fluorescent immunoassays, and surface‐enhanced Raman scattering immunoassays. Moreover, we discuss the benefits and challenges of nanoprobes in immunoassays and provide insights into their future development. This study aims to offer a comprehensive and critical perspective on the role of nanoprobes in the field of immunoassays.

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A high sensitive platinum-modified colloidal gold immunoassay for tenuazonic acid detection based on monoclonal IgG

Cited by 18 publications

References 37 publications

Development of Immunochromatographic Strip for Detection of αB-VxXXIVA-Conotoxin Based on 5E4 Monoclonal Antibody

Development of Immunochromatographic Strip for Detection of αB-VxXXIVA-Conotoxin Based on 5E4 Monoclonal Antibody

Detection of αB-Conotoxin VxXXIVA (αB-CTX) by ic-ELISA Based on an Epitope-Specific Monoclonal Antibody

Advances in nanoprobes‐based immunoassays

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