Acrylamide polymer, as one type of fluid loss agent (FLA), had attracted a strong interest in the applied petroleum research because of its outstanding characteristics of thickening and filtration control. The aim of this study was to synthesize a series of binary FLA using different acrylamide monomers and analyze the effect of monomer structure on the fluid loss control performance of FLA. Three kinds of FLA with the monomer of 2-acrylamido-2-methyl propane sulfonic acid (AMPS) were prepared by polymerization of AMPS with acrylamide (AM), N,N-dimethyl acrylamide (DMAM), N,N-diethyl acrylamide (DEAM), respectively, and characterized by FTIR, 1 H-NMR and 13 C-NMR. The results showed that the FLA prepared by AMPS and DMAM (coded as ADM) showed the best water-retaining capacity, which can be attributed to its moderate rigidity group of-CH 3. It was also revealed that the tolerance of cement slurry containing ADM to Ca 2? was stronger than that to Mg 2? and Na ? .