A highly efficient capillary electrophoresis-based method for size determination of water-soluble CdSe/ZnS core–shell quantum dots

Li, Yongqiang; Wang, Haiqiao; Wang, Jian-Hao; Guan, Liyun; Liu, Bi‐Feng; Zhao, Yuan‐Di; Chen, Hong

doi:10.1016/j.aca.2009.06.004

Cited by 36 publications

(31 citation statements)

References 37 publications

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“…Thus, the successful separation of CdTe nanocrystals according to size with the size span 2.6 nm was recently proposed with the use of capillary gel electrophoresis with polymer as sieving medium [12]. The method was recently extended toward CdSe/ZnS NCs [13]. Next, the reversed electrode polarity stacking mode of CE with the use of anionic surfactant was examined for the enrichment of Au nanoparticles and an increase in the detection of particles, up to 10 3 fold was reported [14–16].…”

Section: Introductionmentioning

confidence: 99%

Characterization of CdSe nanocrystals coated with amphiphiles. A capillary electrophoresis study

2011

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We have synthesized CdSe nanocrystals (NCs) possessing a trioctylphosphine surface passivation layer and modified with amphiphilic molecules to form a surface bilayer. The NCs covered with single amphiphiles are not stable in aqueous solution, but a mixed amphiphilic system is shown to provide stability in solution over several months. The solutions of the modified NCs were characterized by UV-Vis absorbance, photoluminescence, and transmission electron microscopy. An electrophoretic study revealed two operational modes. The first relies on the enrichment of NCs using a micellar plug as a tool. The accumulation of NCs at the plug-electrolyte buffer interface results in a sharp peak. By controlling the electrophoretic conditions, nanocrystals were forced to exit a micellar plug into an electrolyte buffer. We conclude that a system consisting of modified nanocrystals and a micellar plug can act as a mixed pseudomicellar system, where modified nanocrystals play the role of pseudomicelles.FigureElectrophoretic focusing of amphiphile coated CdSe nanocrystals using a micellar plug.Electronic supplementary materialThe online version of this article (doi:10.1007/s00604-011-0727-8) contains supplementary material, which is available to authorized users.

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Section: Introductionmentioning

confidence: 99%

Characterization of CdSe nanocrystals coated with amphiphiles. A capillary electrophoresis study

2011

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“…16,20 CE analysis of a mixture of QD SA and 4 -QD SA complex was carried out using various polyethylene glycol (PEG) (20 kDa) concentrations (0–4%) as sieving medium (Figure S5). At 2% PEG solution gave an optimal separation of QDQD SA and 4 -QD SA complex.…”

Section: Resultsmentioning

confidence: 99%

“…The 2% PEG sieving medium concentration, selected to provide optimal performance, is in agreement with previous reports. 20 …”

Section: Discussionmentioning

confidence: 99%

Ultrasensitive Detection and Quantification of Acidic Disaccharides Using Capillary Electrophoresis and Quantum Dot-Based Fluorescence Resonance Energy Transfer

Chang¹,

Cai²,

Li³

et al. 2013

Anal. Chem.

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Rapid and highly sensitive detection of the carbohydrate components of glycoconjugates is critical for advancing glycobiology. Fluorescence (or Forster) resonance energy transfer (FRET) is commonly used in detection of DNA, in protein structural biology, and in protease assays, but is less frequently applied to glycan analysis due to difficulties in inserting two fluorescent tags into small glycan structures. We report an ultrasensitive method for the detection and quantification of a chondroitin sulfate disaccharide based on FRET, involving a CdSe-ZnS core-shell nanocrystal quantum dot (QD)-streptavidin conjugate donor and a Cy5 acceptor. The disaccharide was doubly labeled with biotin and Cy5. QDs then served to concentrate the target disaccharide, enhancing the overall energy transfer efficiency, with unlinked QDs and Cy5-hydrazide producing nearly zero background signal in capillary electrophoresis using laser-induced fluorescence detection with two different band-pass filters. This method is generally applicable to the ultrasensitive analysis of acidic glycans and offers promise for the high-throughput disaccharide analysis of glycosaminoglycans.

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“…CE has emerged as a powerful tool to separate and characterize different nanoparticles, among which QDs [13][14][15][16][17][18][19][20][21]. In comparison to the classical methods for NP physicochemical characterization, CE presents several advantages, such as high separation efficiency in aqueous, hydro-organic, or organic media, low sample and products consumption, simple sample preparation, accurate quantitative analysis, reduced analysis time, and a high degree of automation [22].…”

Section: Introductionmentioning

confidence: 99%

Characterization of phthalocyanine functionalized quantum dots by dynamic light scattering, laser Doppler, and capillary electrophoresis

et al. 2016

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In this work, we characterized different phtalocyanine-capped core/shell/shell quantum dots (QDs) in terms of stability, ζ-potential, and size at various pH and ionic strengths, by means of capillary electrophoresis (CE), and compared these results to the ones obtained by laser Doppler electrophoresis (LDE) and dynamic light scattering (DLS). The effect of the phthalocyanine metallic center (Zn, Al, or In), the number (one or four), and nature of substituents (carboxyphenoxy- or sulfonated-) of functionalization on the phthalocyanine physicochemical properties were evaluated. Whereas QDs capped with zinc mono-carboxyphenoxy-phtalocyanine (ZnMCPPc-QDs) remained aggregated in the whole analyzed pH range, even at low ionic strength, QDs capped with zinc tetracarboxyphenoxy phtalocyanine (ZnTPPc-QDs) were easily dispersed in buffers at pH equal to or higher than 7.4. QDs capped with aluminum tetrasulfonated phthalocyanine (AlTSPPc-QDs) and indium tetracarboxyphenoxy phthalocyanines (InTCPPc-QDs) were stable in aqueous suspension only at pH higher than 9.0 due to the presence of functional groups bound to the metallic center of the phthalocyanine. The ζ-potential values determined by CE for all the samples decreased when ionic strength increased, being well correlated with the aggregation of the nanoconjugates at elevated salt concentrations. The use of electrokinetic methodologies has provided insights into the colloidal stability of the photosensitizer-functionalized QDs in physiological relevant solutions and thereby, its usefulness for improving their design and applications for photodynamic therapy. Graphical Abstract Schematic illustration of the phthalocyanine capped QDs nanoconjugates and the capillary electrophoresis methods applied for size and ζ-potential characterization.

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A highly efficient capillary electrophoresis-based method for size determination of water-soluble CdSe/ZnS core–shell quantum dots

Cited by 36 publications

References 37 publications

Characterization of CdSe nanocrystals coated with amphiphiles. A capillary electrophoresis study

Characterization of CdSe nanocrystals coated with amphiphiles. A capillary electrophoresis study

Ultrasensitive Detection and Quantification of Acidic Disaccharides Using Capillary Electrophoresis and Quantum Dot-Based Fluorescence Resonance Energy Transfer

Characterization of phthalocyanine functionalized quantum dots by dynamic light scattering, laser Doppler, and capillary electrophoresis

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