A highly selective two‐stage isolation method for the enumeration of <i>Staphylococcus aureus</i> in foods

Isigidi, B.K.; Devriese, Luc; Croegaert, Th.; Hoof, Jan Van

doi:10.1111/j.1365-2672.1989.tb05106.x

Cited by 17 publications

(5 citation statements)

References 17 publications

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“…The acriflavine MICs were in the range 0?8-1?6 mg ml 21 for the four novel isolates, whereas they amounted to 12?5 and 25 mg ml 21 for S. aureus strains (Devriese, 1981). S. aureus strains are unique in being the only staphylococci described, to date, that are resistant to more than 8 mg acriflavine ml 21 -a level used in the selective isolation of this species in food microbiology (Isigidi et al, 1989).…”

mentioning

confidence: 87%

Staphylococcus pseudintermedius sp. nov., a coagulase-positive species from animals

Devriese

Vancanneyt

Baele

et al. 2005

International Journal of Systematic and Evolutionary Microbiology

319

212

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Four staphylococcal isolates from clinical and necropsy specimens from a cat, a dog, a horse and a parrot (Psittacus erithacus timneh) were found to constitute a distinct taxon. 16S rRNA gene sequence analysis revealed that its closest phylogenetic relatives are Staphylococcus intermedius and Staphylococcus delphini. Growth characteristics, biochemical features and DNA-DNA hybridizations demonstrated that the strains differ from these and other known species and that they represent a single, novel Staphylococcus species for which the name Staphylococcus pseudintermedius sp. nov. is proposed. The novel species is commonly confused with S. intermedius in routine diagnostic veterinary bacteriology. Although the strains described were isolated from lesions and show several characteristics typical of pathogenic staphylococci, such as coagulase, DNase and b-haemolysin production, the pathogenic significance of the novel species remains unclear. The type strain, LMG 22219 T (=ON 86 T =CCUG 49543 T ), was isolated from lung tissue of a cat.

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mentioning

confidence: 87%

Staphylococcus pseudintermedius sp. nov., a coagulase-positive species from animals

Devriese

Vancanneyt

Baele

et al. 2005

International Journal of Systematic and Evolutionary Microbiology

319

212

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“…A second factor that influences the numbers of organisms recovered is whether a resuscitation treatment (Mossel & van Netten 1984;Ray 1989) is used. In many instances staphylococci are severely injured, so that recovery is erroneously low if a specimen is plated directly on many currently used selective media (Idziak & Mossel 1980;Mossel et al 1985;Isigidi et al 1989;van Netten et al 1990). Such cells have to be included in counts as when they have recovered from injury their enterotoxinogenicity is unimpaired (Fung & van den Bosch 1975;Smith et al 1984).…”

Section: A a Mossel And P Van Nettenmentioning

confidence: 99%

“…Constituents of the original medium, included to assist repair of staphylococci exposed to adverse intrinsic and extrinsic factors (Baird-Parker & Davenport 1965; Collins-Thompson et al. 1974;Gray et al 1974;Idziak & Mossel 1980), proved to be ineffective in some situations (Mansfield et al 1983;Isigidi et al 1989). Procedures based on solid medium repair (SMR) (Fig.…”

Section: S E L E C T I V E E N U M E R a T I O N Of Staphylococcus Aumentioning

confidence: 99%

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Staphylococcus aureus and related staphylococci in foods: ecology, proliferation, toxinogenesis, control and monitoring

Mossel¹,

Netten

1990

Journal of Applied Bacteriology

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“…other than S. aureus, Bacillus spp., Proteus spp., Enterococcus spp., or Micrococcus spp.) form colonies with a morphology similar to those of S. aureus (Isigidi et al 1989). …”

Section: Discussionmentioning

confidence: 94%

Rapid and Sensitive Detection of Staphylococcus aureus in Food Using Selective Enrichment and Real-Time PCR Targeting a New Gene Marker

et al. 2008

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Staphylococcus aureus is a bacterial pathogen considered a principal etiological agent of food poisoning. The aim of this study was to develop and evaluate a rapid and sensitive method for the detection of S. aureus in food by using selective enrichment and a new species-specific real-time polymerase chain reaction (PCR). Specific primers and a TaqMan probe targeted to specific S. aureus gene encoding for acriflavine resistance protein were designed. The real-time PCR was highly specific for S. aureus with 100% inclusivity and 100% exclusivity determined using 83 S. aureus strains and 64 non-S.-aureus strains. PCR detection limit of 6.8×10 1 and 3.4×10 1 CFU ml −1 were obtained with 100% and 70% detection probability, respectively. The single selective enrichment based on the study of different enrichment conditions was selected and a lysis by boiling was used to obtain bacterial DNA. Out of 112 food samples analyzed, 61 were positive by the PCR-based method and 53 by the standard method. Out of ten food matrices artificially contaminated at a level of 10°CFU g −1 , ten and six were positive by the respective methods. Moreover, 10°CFU 10 g −1 was detected in all ten artificially contaminated samples after a large-scale enrichment using PCR-based detection, in contrast to seven false negative by standard detection. The developed method facilitated the detection of S. aureus on the next day after the sample reception. This method can be used for S. aureus detection as a faster, highly specific, and more sensitive alternative to microbiological method with the potential for providing of improved food-processing hygiene control.

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A highly selective two‐stage isolation method for the enumeration of Staphylococcus aureus in foods

Cited by 17 publications

References 17 publications

Staphylococcus pseudintermedius sp. nov., a coagulase-positive species from animals

Staphylococcus pseudintermedius sp. nov., a coagulase-positive species from animals

Staphylococcus aureus and related staphylococci in foods: ecology, proliferation, toxinogenesis, control and monitoring

Rapid and Sensitive Detection of Staphylococcus aureus in Food Using Selective Enrichment and Real-Time PCR Targeting a New Gene Marker

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