A highly sensitive capillary electrophoresis immunoassay strategy based on dual‐labeled gold nanoparticles enhancing chemiluminescence for the detection of prostate‐specific antigen

Li, Shuting; Shi, Min; Zhao, Jingjin; Zhang, Liangliang; Huang, Yong; Zhao, Shulin

doi:10.1002/elps.201600396

Cited by 15 publications

(12 citation statements)

References 42 publications

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“…Likewise, another work was reported for the enhanced separation of the three isomers of dihydroxybenzene ( o‐ , m‐ , p ‐dihydroxybenzene) by CE using AuNPs as stationary phase. Under the optimum conditions, a good resolution of three dihydroxybenzene isomers was obtained with wide linear ranges and better LOD . In another similar work, effective separations of synthetic peptides mixture were obtained on the AuNP‐coated capillaries.…”

Section: Gold Npsmentioning

confidence: 93%

Recent advances in nanomaterial‐based capillary electrophoresis

et al. 2019

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This review gives a summary of applications of different nanomateials, such as gold nanoparticles (AuNPs), carbon‐based nanoparticles, magnetic nanoparticles (MNPs), and nano‐sized metal organic frameworks (MOFs), in electrophoretic separations. This review also emphasizes the recent works in which nanoparticles (NPs) are used as pseudostationary phase (PSP) or immobilized on the capillary surface for enhancement of separation in CE, CEC, and microchips electrophoresis.

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Section: Gold Npsmentioning

confidence: 93%

Recent advances in nanomaterial‐based capillary electrophoresis

et al. 2019

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“…Several articles about the immobilization of an Ab/Ag onto a capillary wall , magnetic beads , silica particles , glass beads , latex particles , or gold nanoparticles have been published. More detailed information on applications of heterogeneous CEIA are summarized in Table .…”

Section: Heterogeneous Ce Immunoassaysmentioning

confidence: 99%

Capillary electrophoresis–based immunoassay and aptamer assay: A review

2020

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Capillary electrophoresis-based immunoassay and aptamer assay: A reviewOver the last two decades, the group of techniques called affinity probe CE has been widely used for the detection and the determination of several types of biomolecules with high sensitivity. These techniques combine the low sample consumption and high separation power of CE with the selectivity of the probe to the target molecule. The assays can be defined according to the type of probe used: CE immunoassays, with an antibody as the probe, or aptamer-based CE, with an aptamer as the probe. Immunoassays are generally divided into homogeneous and heterogeneous groups, and homogeneous variant can be further performed in competitive or noncompetitive formats. Interacting partners are free in solution at homogeneous assay, as opposed to heterogeneous analyses, where one of them is immobilized onto a solid support. Highly sensitive fluorescence, chemiluminescence or electrochemical detections were typically used in this type of study. The use of the aptamers as probes has several advantages over antibodies such as shorter generation time, higher thermal stability, lower price, and lower variability. The aptamer-based CE technique was in practice utilized for the determination of proteins in biological fluids and environmentally or clinically important small molecules. Both techniques were also transferred to microchip. This review is focused on theoretical principles of these techniques and a summary of their applications in research.Abbreviations: Ag, antigen; CEIA, CE immunoassay; MCE, microchip CE; SELEX, systematic evolution of ligands by exponential enrichment and they are produced by the immune system of living organisms. Ag that start the immunological response are also called immunogens. These Ab-Ag complexes are stabilized by dipole-dipole, van der Waals or hydrophobic interactions and hydrogen bonds [5,6].The most often used immunoassays are ELISA with Ab or Ag immobilized onto solid support (plates, glass fibers, or plastic tubes), immunofluorescence assay, fluorescence polarization immunoassay, luminescence immunoassay, microparticle enzyme immunoassay, and biosensors. The major disadvantages of immunoassays are the tedious process, time-consuming reactions, and problems with reproducibility and nonspecific interactions [6].CE is a powerful analytical method for the separation of a wide range of molecules. The coupling of CE and immunoassay in the new CE immunoassay (CEIA) technique provided both separation power and specificity of interactions. Other advantages are the possibility of multianalyte analyses, automation, low sample consumption, high speed, flexibility, a lower amount of false-positive results, and also the possibility of connection with highly sensitive detectors [5].In CEIA, the Fab fragments of Ab containing Ag-specific regions are more often used than the whole monoclonal and Color online: See article online to view Figs. 4 and 7 in color.

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“…An interesting use of gold nano‐particles that were dual labeled with both horseradish peroxidase and anti‐prostate specific antigen (PSA) antibody was developed by Li et al. . The particles were added to the samples to capture the available PSA to form an immune complex that was separated and detected by CE‐CL via the horseradish peroxidase technique.…”

Section: Applications In Different Clinical Fieldsmentioning

confidence: 99%

Recent advances in CE and microchip‐CE in clinical applications: 2014 to mid‐2017

Phillips

2017

Electrophoresis

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Recent advances in CE and microchip-CE in clinical applications: 2014 to mid-2017CE and microchip CE (ME) are powerful tools for the analysis of a number of different analytes and have been applied to a variety of clinical fields and human samples. This review will present an overview of the most recent applications of these techniques to different areas of clinical medicine during the period of 2014 to mid-2017. CE and ME have been applied to clinical chemistry, drug detection and monitoring, hematology, infectious diseases, oncology, endocrinology, neonatology, nephrology, and genetic screening. Samples examined range from serum, plasma, and urine to lest utilized materials such as tears, cerebral spinal fluid, sweat, saliva, condensed breath, single cells, and biopsy tissue. Examples of clinical applications will be given along with the various detection systems employed.

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A highly sensitive capillary electrophoresis immunoassay strategy based on dual‐labeled gold nanoparticles enhancing chemiluminescence for the detection of prostate‐specific antigen

Cited by 15 publications

References 42 publications

Recent advances in nanomaterial‐based capillary electrophoresis

Recent advances in nanomaterial‐based capillary electrophoresis

Capillary electrophoresis–based immunoassay and aptamer assay: A review

Recent advances in CE and microchip‐CE in clinical applications: 2014 to mid‐2017

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