A highly sensitive detection of chloramphenicol based on chemiluminescence immunoassays with the cheap functionalized Fe₃O₄@SiO₂ magnetic nanoparticles

Abstract

A strategy has been applied to chloramphenicol (CAP) detection with chemiluminescence immunoassays (CLIA) based on cheap functionalized Fe O @SiO magnetic nanoparticles (Fe-MNPs). The strategy that bovine serum albumin (BSA) was immobilized on cheap functionalized Fe-MNPs and that the CAP molecules were then immobilized on BSA, avoided the long process of dialysis for preparation of the BSA-CAP conjugates. The samples were detected for both methods that utilized two different kinds of functionalized Fe-MNPs (amine-functionalized Fe O @SiO and carboxylic acid-functionalized Fe O @SiO ). The sensitivities and limits of detection (LODs) of the two methods were obtained and compared based on inhibition curves. The 50% inhibition concentrations (IC ) values of the two methods were about 0.024 ng ml and 0.046 ng ml respectively and LODs were approximately 0.0002 ng ml and 0.001 ng ml respectively. These methods were much more sensitive than that of any traditional enzyme-linked immunosorbent assay (ELISA) previously reported. Therefore, such chemiluminescence methods could be easily adapted for small molecule detection in a variety of foods using Fe-MNPs.

A label-free silicon-based spherical nucleic acid enzyme (SNAzyme) for ultrasensitive chemiluminescence detection of acute myocardial infarction-related nucleic acids

“…As a result, various organic functional groups can be further modified on the surface by organosilanes and crosslinking agents, such as amino, 15 carboxyl, 16 thiol, 17 aldehyde, 18 and epoxy groups, 19 etc. After the modification of SiO 2 , biological molecules such as nucleic acids, 20 proteases 21 and antibodies 22 as well as functional molecules such as fluorescent 23 and quantum dots 24 can be loaded by electrostatic adsorption or covalent reaction. Luo et al have simultaneously labeled ABEI and DNA onto the SiO 2 surface to achieve the construction of a CL probe.…”

Development of a multicomponent immunochromatographic test system for the detection of fluoroquinolone and amphenicol antibiotics in dairy products

“…16,17 ELISA protocols for the detection of CIP and CAP in food products have been described in various investigations. [18][19][20][21] In comparison to ELISA, ICA is methodologically simple, does not require any equipment, and is rapid (the assay results can be visually assessed within 5-10 min). 22 Features of the ICAs proposed for the detection of antibiotics in milk are summarized in Table 1.…”

Highly Specific Chemiluminescence Immunoassay for the Determination of Chloramphenicol in Cosmetics

“…Chloramphenicol is usually performed as a broad-spectrum antibiotic with effective antimicrobial activity. It can be usually against a spread variety of Gram-positive and Gram-negative bacteria, including the anaerobic organisms [3]. Due to the high activity, chloramphenicol is widely applied in livestock, aquiculture, and cosmetics industries for the prevention and treatment of bacterial infection.…”