A highly sensitive detection of carbendazim pesticide in food based on the upconversion-MnO2 luminescent resonance energy transfer biosensor

Ouyang, Qin; Wang, Li; Ahmad, Waqas; Rong, Yawen; Li, Huanhuan; Hu, Yuqian; Chen, Quansheng

doi:10.1016/j.foodchem.2021.129157

Cited by 109 publications

(39 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Only few optical sensors for carbendazim detection have been reported in the literature. These include a sensor based on surface-enhanced Raman scattering technology, which involves cyclodextrin inclusion complexes on gold nanorods as recognition element [ 32 ], a sensor based on luminescence resonance energy transfer from aptamer-labeled upconversion nanoparticles to manganese dioxide nanosheets that act as an acceptor [ 33 ], and a surface plasmon resonance-based immunosensor [ 34 ]. The immunosensor proposed herein is the first optical sensor based on white light reflectance spectroscopy, dedicated to the determination of carbendazim in foodstuff.…”

Section: Resultsmentioning

confidence: 99%

Fast and Sensitive Determination of the Fungicide Carbendazim in Fruit Juices with an Immunosensor Based on White Light Reflectance Spectroscopy

et al. 2021

View full text Add to dashboard Cite

Carbendazim is a systemic benzimidazole-type fungicide with broad-spectrum activity against fungi that undermine food products safety and quality. Despite its effectiveness, carbendazim constitutes a major environmental pollutant, being hazardous to both humans and animals. Therefore, fast and reliable determination of carbendazim levels in water, soil, and food samples is of high importance for both food industry and public health. Herein, an optical biosensor based on white light reflectance spectroscopy (WLRS) for fast and sensitive determination of carbendazim in fruit juices is presented. The transducer is a Si/SiO2 chip functionalized with a benzimidazole conjugate, and determination is based on a competitive immunoassay format. Thus, for the assay, a mixture of an in-house developed rabbit polyclonal anti-carbendazim antibody with the standards or samples is pumped over the chip, followed by biotinylated secondary antibody and streptavidin. The WLRS platform allows for real-time monitoring of biomolecular interactions carried out onto the Si/SiO2 chip by transforming the shift in the reflected interference spectrum caused by the immunoreaction to effective biomolecular adlayer thickness. The sensor is able to detect 20 ng/mL of carbendazim in fruit juices with high accuracy and precision (intra- and inter-assay CVs ≤ 6.9% and ≤9.4%, respectively) in less than 30 min, applying a simple sample treatment that alleviates any “matrix-effect” on the assay results and a 60 min preincubation step for improving assay sensitivity. Excellent analytical characteristics and short analysis time along with its small size render the proposed WLRS immunosensor ideal for future on-the-spot determination of carbendazim in food and environmental samples.

show abstract

Section: Resultsmentioning

confidence: 99%

Fast and Sensitive Determination of the Fungicide Carbendazim in Fruit Juices with an Immunosensor Based on White Light Reflectance Spectroscopy

et al. 2021

View full text Add to dashboard Cite

show abstract

“…Specificity, recognizing the target molecule, is essential in the performance evaluation (33). The accuracy of the assay method is mainly based on the specificity of the CBZ aptamer (2).…”

Section: Specificitymentioning

confidence: 99%

A Competitive Assay Based on Dual-Mode Au@Pt-DNA Biosensors for On-Site Sensitive Determination of Carbendazim Fungicide in Agricultural Products

Zhai

Liu

et al. 2022

Front. Nutr.

View full text Add to dashboard Cite

Carbendazim (CBZ), a systemic, broad-spectrum benzimidazole fungicide, is widely used to control fungal diseases in agricultural products. Its residues might pose risks to human health and the environment. Therefore, it is warranted to establish a rapid and reliable method for its residual quantification. Herein, we proposed a competitive assay that combined aptamer (DNA) specific recognition and bimetallic nanozyme gold@platinum (Au@Pt) catalysis to trace the CBZ residue. The DNA was labeled onto bimetallic nanozyme Au@Pt surface to produce Au@Pt probes (Au@Pt-DNA). The magnetic Fe3O4 was functionalized with a complementary strand of DNA (C-DNA) to form Fe3O4 probes (Fe3O4-C-DNA). Subsequently, the CBZ and the Fe3O4 probes competitively react with Au@Pt probes to form two Au@Pt-DNA biosensors (Au@Pt-ssDNA-CBZ and Au@Pt-dsDNA-Fe3O4). The Au@Pt-ssDNA-CBZ biosensor was designed for qualitative analysis through a naked-eye visualization strategy in the presence of CBZ. Meanwhile, Au@Pt-dsDNA-Fe3O4 biosensor was developed to quantitatively analyze CBZ using a multifunctional microplate reader. A competitive assay based on the dual-mode Au@Pt-DNA biosensors was established for onsite sensitive determination of CBZ. The limit of detection (LOD) and recoveries of the developed assay were 0.038 ng/mg and 71.88-110.11%, with relative standard deviations (RSDs) ranging between 3.15 and 10.91%. The assay demonstrated a good correlation with data acquired from liquid chromatography coupled with mass spectrometry/mass spectrometry analysis. In summary, the proposed competitive assay based on dual-mode Au@Pt-DNA biosensors might have a great potential for onsite sensitive detection of pesticides in agro-products.

show abstract

“…Biosensor is an emerging and promising technology in detecting food contaminants such as pesticides, and a variety of biosensors have been developed in recent years (Majdinasab et al 2018(Majdinasab et al , 2019. For example, Ouyang et al (Ouyang et al 2021) developed a sensitive biosensor to detect carbendazim pesticide residues based on luminescent resonance energy transfer from aptamer-labelled upconversion nanoparticles to manganese dioxide nanosheets. Capobianco et al (Capobianco et al 2021) developed an enzyme-linked immunoelectrochemical biosensor to detect pathogenic bacteria in large volume food samples without subsampling.…”

Section: Rapid Detection Of Contaminantsmentioning

confidence: 99%

Environment and food safety: a novel integrative review

Jiang

Wang

et al. 2021

Environ Sci Pollut Res

View full text Add to dashboard Cite

Environment protection and food safety are two critical issues in the world. In this review, a novel approach which integrates statistical study and subjective discussion was adopted to review recent advances on environment and food safety. Firstly, a scientometric-based statistical study was conducted based on 4904 publications collected from the Web of Science Core Collection database. It was found that the research on environment and food safety was growing steadily from 2001 to 2020. Interestingly, the statistical analysis of most-cited papers, titles, abstracts, keywords, and research areas revealed that the research on environment and food safety was diverse and multidisciplinary. In addition to the scientometric study, strategies to protect environment and ensure food safety were critically discussed, followed by a discussion on the emerging research topics, including emerging contaminates (e.g., microplastics), rapid detection of contaminants (e.g., biosensors), and environment friendly food packaging materials (e.g., biodegradable polymers). Finally, current challenges and future research directions were proposed. Supplementary Information The online version contains supplementary material available at 10.1007/s11356-021-16069-6.

show abstract

A highly sensitive detection of carbendazim pesticide in food based on the upconversion-MnO2 luminescent resonance energy transfer biosensor

Cited by 109 publications

References 39 publications

Fast and Sensitive Determination of the Fungicide Carbendazim in Fruit Juices with an Immunosensor Based on White Light Reflectance Spectroscopy

Fast and Sensitive Determination of the Fungicide Carbendazim in Fruit Juices with an Immunosensor Based on White Light Reflectance Spectroscopy

A Competitive Assay Based on Dual-Mode Au@Pt-DNA Biosensors for On-Site Sensitive Determination of Carbendazim Fungicide in Agricultural Products

Environment and food safety: a novel integrative review

Contact Info

Product

Resources

About