A highly sensitive enzyme-linked immunoassay for serum free prostate specific antigen (f-PSA)

Matsumoto, Kyoichi; Konishi, Noboru; Hiasa, Yoshio; Kimura, E; Takahashi, Yujiro; Shinohara, K.; Samori, Tomohiro

doi:10.1016/s0009-8981(98)00208-3

Cited by 31 publications

(12 citation statements)

References 28 publications

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“…Thus, developing a simple, sensitivity and reliable method to detect PSA is important for patient. Until now, several methods including enzyme-linked immunosorbent-assay (ELISA) [5], Chemiluminescent immuno-assay [6], bioluminescent immunoassay [7] and electrochemical immunoassay [8,9] have been conducted on clinical serum sample measurements. Although those methods are reliable and precise, it is time consuming, requires complex equipment and trained personal, not convenient for large-scale use in developing country [10].…”

Section: Introductionmentioning

confidence: 99%

Sandwich immunoassay for the prostate specific antigen using a micro-fluxgate and magnetic bead labels

et al. 2016

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We describe a micro fluxgate based device with rectangular magnetic core for the determination of prostate specific antigen (PSA) labeled with Dynabeads. A sandwich immunoassay was employed where PSA is captured on a gold film modified with a self-assembled monolayer of antibody. The secondary antibody is labeled with Dynabeads. By applying a DC magnetic fields in the range of 460 to 700 μT, PSA can be detected with detection limit as low as 0.1 ng mL −1 . This micro fluxgate-based assay offers the advantages of miniaturization, simple and conveniently manipulation, reusability and stability. In our perception, it offers a viable approach towards clinical determination of PSA or other biomarkers.

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Section: Introductionmentioning

confidence: 99%

Sandwich immunoassay for the prostate specific antigen using a micro-fluxgate and magnetic bead labels

et al. 2016

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“…Adoption of antibodies for the BGM assay can improve the assay’s sensitivity to a similar level achieved by immunoassays. For example, a BGM-based sandwich assay for Prostate Specific Antigen has been demonstrated with a LoD at 400 pg/mL [9], which is closer to the LoD of existing ELISA assay (typically in the 10–100 pg/mL range) [21–23] ( see Note 10 ). Negative controls using human serum albumin result in negligibly low readings from the BGM under otherwise identical conditions.…”

Section: Methodsmentioning

confidence: 90%

Detection of Protein Biomarker Using a Blood Glucose Meter

Lan

Xiang

2014

Methods in Molecular Biology

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mHeath technologies are recognized to play important roles in the future of personal care and medicine. However, their full potentials have not been reached, as most of current technologies are restricted to monitoring physical and behavioral parameters, such as body temperature, heart rate, blood pressure, and physical movement, while direct monitoring of biomarkers in body fluids can provide much more accurate and useful information for medical diagnostics. A major barrier to realizing the full potential of mHealth is the high costs and long cycles of developing mHealth devices capable of monitoring biomarkers in body fluids. To lower the costs and shorten the developmental cycle, we have demonstrated the leveraging of the most successful portable medical monitoring device on the market, the blood glucose meter (BGM), with FDA-approved smartphone technologies that allow for wireless transmission and remote monitoring of a wide range of non-glucose targets. In this protocol, an aptamer-based assay for quantification of interferon-γ (IFN-γ) using an off-the-shelf BGM is described. In this assay, an aptamer-based target recognition system is employed. When IFN-γ binds to the aptamer, it triggers the release of a reporter enzyme, invertase, which can catalyze the conversion of sucrose (not detected by BGM) to glucose. The glucose being produced is then detected using a BGM. The system mimics a competitive enzyme-linked immunosorbent assay (ELISA), where the traditional immunoassay is replaced by an aptamer binding assay; the reporter protein is replaced by invertase, and finally the optical or fluorescence detector is replaced with widely available BGMs.

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“…But most of them need long measurement time, high cost, and multiple equipment, and they are highly qualified and are not suitable for massive decentralized testing. Accordingly, it is still a challenge to develop simple, low‐cost for sensitive determination of PSA levels …”

Section: Introductionmentioning

confidence: 99%

A novel electroconductive interface based on Fe₃O₄ magnetic nanoparticle and cysteamine functionalized AuNPs: Preparation and application as signal amplification element to minoring of antigen‐antibody immunocomplex and biosensing of prostate cancer

Farshchi

Hasanzadeh

Mokhtarzadeh

2019

J of Molecular Recognition

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In this study, a novel electroconductive interface was prepared based on Fe3O4 magnetic nanoparticle and cysteamine functionalized gold nanoparticle. The engineered interface was used as signal amplification substrate in the electrochemical analysis of antibody‐antigen binding. For this purpose, biotinilated‐anti‐prostate‐specific antigen (PSA) antibody was bioconjugated with iron oxide magnetic nanoparticles (Fe3O4) and drop‐casted on the surface of glassy carbon electrode (GCE). Also, secondary antibody (HRP‐Ab2) encapsulated on gold nanoparticles caped by cysteamine was immobilized on the surface of GCE modified electrode. A transmission electron microscopy images shows that a sandwich immunoreaction was done and binding of Ab1 and Ab2 performed successfully. Various parameters of immunoassay, including the loading of magnetic nanoparticles, the amount of gold nanoparticle conjugate, and the immunoreaction time, were optimized. The detection limit of 0.001 μg. L−1 of PSA was obtained under optimum experimental conditions. It is found that such magneto‐bioassay could be readily used for simultaneous parallel detection of multiple proteins by using multiple inorganic metal nanoparticle tracers and are expected to open new opportunities for early stage diagnosis of cancer in near future.

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A highly sensitive enzyme-linked immunoassay for serum free prostate specific antigen (f-PSA)

Cited by 31 publications

References 28 publications

Sandwich immunoassay for the prostate specific antigen using a micro-fluxgate and magnetic bead labels

Sandwich immunoassay for the prostate specific antigen using a micro-fluxgate and magnetic bead labels

Detection of Protein Biomarker Using a Blood Glucose Meter

A novel electroconductive interface based on Fe₃O₄ magnetic nanoparticle and cysteamine functionalized AuNPs: Preparation and application as signal amplification element to minoring of antigen‐antibody immunocomplex and biosensing of prostate cancer

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A highly sensitive enzyme-linked immunoassay for serum free prostate specific antigen (f-PSA)

Cited by 31 publications

References 28 publications

Sandwich immunoassay for the prostate specific antigen using a micro-fluxgate and magnetic bead labels

Sandwich immunoassay for the prostate specific antigen using a micro-fluxgate and magnetic bead labels

Detection of Protein Biomarker Using a Blood Glucose Meter

A novel electroconductive interface based on Fe3O4 magnetic nanoparticle and cysteamine functionalized AuNPs: Preparation and application as signal amplification element to minoring of antigen‐antibody immunocomplex and biosensing of prostate cancer

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A novel electroconductive interface based on Fe₃O₄ magnetic nanoparticle and cysteamine functionalized AuNPs: Preparation and application as signal amplification element to minoring of antigen‐antibody immunocomplex and biosensing of prostate cancer