A highly sensitive fluorescence-enhanced aptasensor based on polyAn-aptamer nanostructure

Jiang, Liying; Hang, Xinxin; Zhang, Pei; Zhang, Jitao; Wang, Yanfeng; Wang, Wei; Ren, Linjiao

doi:10.1016/j.microc.2019.04.061

Cited by 12 publications

(13 citation statements)

References 26 publications

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“…Jiang et al, developed a highly sensitive fluorescence-enhanced aptasensor based on a polyA n -aptamer nanostructure for the detection of adenosine triphosphate (ATP) [ 82 ]. Their approach involved optimizing the distance between fluorophores, in this case fluorescein (FAM), and the AuNP to enhance fluorescence by adjusting the number of adenosine bases at the 5′ end of a polyA n -aptamer.…”

Section: Aptameric Structure-based Plasmon Biosensormentioning

confidence: 99%

Recent Advances in DNA Nanotechnology for Plasmonic Biosensor Construction

et al. 2022

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Since 2010, DNA nanotechnology has advanced rapidly, helping overcome limitations in the use of DNA solely as genetic material. DNA nanotechnology has thus helped develop a new method for the construction of biosensors. Among bioprobe materials for biosensors, nucleic acids have shown several advantages. First, it has a complementary sequence for hybridizing the target gene. Second, DNA has various functionalities, such as DNAzymes, DNA junctions or aptamers, because of its unique folded structures with specific sequences. Third, functional groups, such as thiols, amines, or other fluorophores, can easily be introduced into DNA at the 5′ or 3′ end. Finally, DNA can easily be tailored by making junctions or origami structures; these unique structures extend the DNA arm and create a multi-functional bioprobe. Meanwhile, nanomaterials have also been used to advance plasmonic biosensor technologies. Nanomaterials provide various biosensing platforms with high sensitivity and selectivity. Several plasmonic biosensor types have been fabricated, such as surface plasmons, and Raman-based or metal-enhanced biosensors. Introducing DNA nanotechnology to plasmonic biosensors has brought in sight new horizons in the fields of biosensors and nanobiotechnology. This review discusses the recent progress of DNA nanotechnology-based plasmonic biosensors.

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Section: Aptameric Structure-based Plasmon Biosensormentioning

confidence: 99%

Recent Advances in DNA Nanotechnology for Plasmonic Biosensor Construction

et al. 2022

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“…In particular, an improvement of 60% may seem insignicant as compared to the previously reported uorescence enhancement studies where the improvement was oen in the range of tens of fold. 35,36 It is important to note that the previously reported studies also oen involved the synthesis of nanomaterials, new aptamers and uorescence dyes as well as alternative bindingquenching mechanisms. 35,36 However in this case, the improvement was achieved by simply pairing an existing aptamer with an appropriate ssDNA probe.…”

Section: Potential and Limitationsmentioning

confidence: 99%

“…35,36 It is important to note that the previously reported studies also oen involved the synthesis of nanomaterials, new aptamers and uorescence dyes as well as alternative bindingquenching mechanisms. 35,36 However in this case, the improvement was achieved by simply pairing an existing aptamer with an appropriate ssDNA probe. It is also reasonable to suggest that similar improvement can be achieved by simultaneously pairing the aptamer with all three ssDNA probes and hence circumvent the need to select amongst them.…”

Section: Potential and Limitationsmentioning

confidence: 99%

Single-stranded DNA probe paired aptasensor with extra dye binding sites to enhance its fluorescence response in the presence of a target compound

Cho

Lim²,

Chua³

et al. 2021

RSC Adv.

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“…Until now, many papers concerning the preparation of aptamer-based biosensors have been published. These aptasensors were constructed using various methodologies, such as optical aptasensors (fluorescence-based optical aptasensors and colorimetric-based optical aptasensors), electrochemical aptasensors, chemiluminescent aptasensors, and surface-enhanced Raman scattering (SERS) aptasensors [ [95] , [96] , [97] , [98] , [99] ]. However, most of the principles of these aptasensors have some versatile or analogous elements.…”

Section: Aptamer-based Biosensormentioning

confidence: 99%