A Highly Sensitive Impedimetric DNA Biosensor Based on Hollow Silica Microspheres for Label-Free Determination of E. coli

Ariffin, Eda Yuhana; Heng, Lee Yook; Tan, Ling Ling; Karim, Nurul Huda Abd; Hasbullah, Siti Aishah

doi:10.3390/s20051279

Cited by 36 publications

(14 citation statements)

References 45 publications

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“…The N element increases in Fig. 7 d due to the formation of double-stranded DNA 34 . The process of DNA hybridisation is successful and was proved by the addition of a nitrogen element in Fig.…”

Section: Resultsmentioning

confidence: 93%

Hexaferrocenium tri[hexa(isothiocyanato)iron(III)] trihydroxonium complex as a new DNA intercalator for electrochemical DNA biosensor

Ariffin

Zakariah

Ruslin

et al. 2021

Sci Rep

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Ferrocene or ferrocenium has been widely studied in the field of organometallic complexes because of its stable thermodynamic, kinetic and redox properties. Novel hexaferrocenium tri[hexa(isothiocyanato)iron(III)]trihydroxonium (HexaFc) complex was the product from the reaction of ferrocene, maleic acid and ammonium thiocyanate and was confirmed by elemental analysis CHNS, FTIR and single crystal X-ray crystallography. In this study, HexaFc was used for the first time as an electroactive indicator for porcine DNA biosensor. The UV–Vis DNA titrations with this compound showed hypochromism and redshift at 250 nm with increasing DNA concentrations. The binding constant (Kb) for HexaFc complex towards CT-DNA (calf-thymus DNA) was 3.1 × 104 M−1, indicated intercalator behaviour of the complex. To test the usefulness of this complex for DNA biosensor application, a porcine DNA biosensor was constructed. The recognition probes were covalently immobilised onto silica nanospheres (SiNSs) via glutaraldehyde linker on a screen-printed electrode (SPE). After intercalation with the HexaFc complex, the response of the biosensor to the complementary porcine DNA was measured using differential pulse voltammetry. The DNA biosensor demonstrated a linear response range to the complementary porcine DNA from 1 × 10−6 to 1 × 10−3 µM (R2 = 0.9642) with a limit detection of 4.83 × 10−8 µM and the response was stable up to 23 days of storage at 4 °C with 86% of its initial response. The results indicated that HexaFc complex is a feasible indicator for the DNA hybridisation without the use of a chemical label for the detection of porcine DNA.

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Section: Resultsmentioning

confidence: 93%

Hexaferrocenium tri[hexa(isothiocyanato)iron(III)] trihydroxonium complex as a new DNA intercalator for electrochemical DNA biosensor

Ariffin

Zakariah

Ruslin

et al. 2021

Sci Rep

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“…Ariffin et al introduced an electrochemical DNA biosensor for E. coli detection [ 65 ]. The genosensor employs silica microspheres that was treated with APTES that was applied to screen-printed electrodes with AuNPs.…”

Section: Pathogen Detection With Electrochemical Biosensorsmentioning

confidence: 99%

Pathogen detection with electrochemical biosensors: Advantages, challenges and future perspectives

Kaya

Çetin²,

Azimzadeh

et al. 2021

Journal of Electroanalytical Chemistry

152

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“…Yuhana et al developed a new label-free electrochemical DNA biosensor for detecting E. coli bacteria in environmental water samples. The aminated DNA probe was mounted on 3-aminopropyltriethoxysilane-functionalized hollow silica microspheres (HSMs) and placed onto a screen-printed electrode (SPE) carbon paste with integrated gold NPs [92]. The biosensor's selectivity and specificity were improved.…”

Section: Silica Npsmentioning

confidence: 99%

Nanomaterials in the Management of Gram-Negative Bacterial Infections

et al. 2021

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The exploration of multiplexed bacterial virulence factors is a major problem in the early stages of Escherichia coli infection therapy. Traditional methods for detecting Escherichia coli (E. coli), such as serological experiments, immunoassays, polymerase chain reaction, and isothermal microcalorimetry have some drawbacks. As a result, detecting E. coli in a timely, cost-effective, and sensitive manner is critical for various areas of human safety and health. Intelligent devices based on nanotechnology are paving the way for fast and early detection of E. coli at the point of care. Due to their specific optical, magnetic, and electrical capabilities, nanostructures can play an important role in bacterial sensors. Another one of the applications involved use of nanomaterials in fighting microbial infections, including E. coli mediated infections. Various types of nanomaterials, either used directly as an antibacterial agent such as metallic nanoparticles (NPs) (silver, gold, zinc, etc.), or as a nanocarrier to deliver and target the antibiotic to the E. coli and its infected area. Among different types, polymeric NPs, lipidic nanocarriers, metallic nanocarriers, nanomicelles, nanoemulsion/ nanosuspension, dendrimers, graphene, etc. proved to be effective vehicles to deliver the drug in a controlled fashion at the targeted site with lower off-site drug leakage and side effects.

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A Highly Sensitive Impedimetric DNA Biosensor Based on Hollow Silica Microspheres for Label-Free Determination of E. coli

Cited by 36 publications

References 45 publications

Hexaferrocenium tri[hexa(isothiocyanato)iron(III)] trihydroxonium complex as a new DNA intercalator for electrochemical DNA biosensor

Hexaferrocenium tri[hexa(isothiocyanato)iron(III)] trihydroxonium complex as a new DNA intercalator for electrochemical DNA biosensor

Pathogen detection with electrochemical biosensors: Advantages, challenges and future perspectives

Nanomaterials in the Management of Gram-Negative Bacterial Infections

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