A highly sensitive impedimetric label free immunosensor for Ochratoxin measurement in cocoa beans

Malvano, Francesca; Albanese, Donatella; Pilloton, Roberto; Matteo, Marisa Di

doi:10.1016/j.foodchem.2016.06.034

Cited by 54 publications

(27 citation statements)

References 18 publications

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“…The cysteamine layers, as well as the consecutive immobilization of anti-OTA and ethanolamine, caused a significant decrease of the electron transfer rate, measured by CV, with a lowering of both anodic and cathodic peaks due to hindering effects of the layers [ 29 , 30 ] ( Figure 5 ).…”

Section: Resultsmentioning

confidence: 99%

Impedimetric Label-Free Immunosensor on Disposable Modified Screen-Printed Electrodes for Ochratoxin A

et al. 2016

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An impedimetric label-free immunosensor on disposable screen-printed carbon electrodes (SPCE) for quantitative determination of Ochratoxin A (OTA) has been developed. After modification of the SPCE surface with gold nanoparticles (AuNPs), the anti-OTA was immobilized on the working electrode through a cysteamine layer. After each coating step, the modified surfaces were characterized by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The capacitance was chosen as the best parameter that describes the reproducible change in electrical properties of the electrode surface at different OTA concentrations and it was used to investigate the analytical parameters of the developed immunosensor. Under optimized conditions, the immunosensor showed a linear relationship between 0.3 and 20 ng/mL with a low detection limit of 0.25 ng/mL, making it suitable to control OTA content in many common food products. Lastly, the immunosensor was used to measure OTA in red wine samples and the results were compared with those registered with a competitive ELISA kit. The immunosensor was sensitive to OTA lower than 2 μg/kg, which represents the lower acceptable limit of OTA established by European legislation for common food products.

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Section: Resultsmentioning

confidence: 99%

Impedimetric Label-Free Immunosensor on Disposable Modified Screen-Printed Electrodes for Ochratoxin A

et al. 2016

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“…Beer (spiked at µg/l) and wine (spiked at 1 µg/l) samples have been successfully analysed with this device, needing a total analysis time of 30 min per sample. Malvano et al (2016) developed an impedimetric label-free immunosensor for the detection of OTA. Oriented and nonoriented antibody immobilisation methods were compared.…”

Section: Ochratoxinsmentioning

confidence: 99%

Developments in mycotoxin analysis: an update for 2017-2018

Tittlemier¹,

Cramer

Dall’Asta

et al. 2019

WMJ

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This review summarises developments that have been published in the period from mid-2017 to mid-2018 on the analysis of various matrices for mycotoxins. Analytical methods to determine aflatoxins, Alternaria toxins, ergot alkaloids, fumonisins, ochratoxins, patulin, trichothecenes, and zearalenone are covered in individual sections. Advances in sampling strategies are discussed in a dedicated section, as are methods used to analyse botanicals and spices, and newly developed comprehensive liquid chromatographic-mass spectrometric based multi-mycotoxin methods. This critical review aims to briefly discuss the most important recent developments and trends in mycotoxin determination as well as to address limitations of the presented methodologies.

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“…The European Committee Regulations (ECR) has established the maximum limits of OTA in cereals at 5 µg·kg −1 (raw cereals grains) and 3 µg·kg −1 in processed cereals products for direct human consumption (EC) No 123-2005. Some methods based on electrochemical biosensors for the determination of OTA in different matrices are summarized in Table 1 [61][62][63][64]. A particularly appealing enzymatic-free design was developed by Bulbul et al [62] for OTA determination involving the synergistic contribution of the catalase activity of a nanoceria (nCe) tag and the peroxidase like activity of graphene oxide (GO) used as electrode modifier for amplification purposes.…”

Section: Introductionmentioning

confidence: 99%

“…These results agreed with the more uniform and homogeneous antibody layer in the oriented immunosensor that favors higher antigen-binding capacity and sensitivity. Consequently, the oriented design was used for application to real samples, and was specifically validated by analyzing spiked cocoa bean samples [64].…”

Section: Introductionmentioning

confidence: 99%

Electrochemical Affinity Biosensors in Food Safety

2017

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Safety and quality are key issues of today's food industry. Since the food chain is becoming more and more complex, powerful analytical methods are required to verify the performance of food safety and quality systems. Indeed, such methods require high sensitivity, selectivity, ability for rapid implementation and capability of automatic screening. Electroanalytical chemistry has, for decades, played a relevant role in food safety and quality assessment, taking more and more significance over time in the solution of analytical problems. At present, the implementation of electrochemical methods in the food is evident. This is in a large part due to the relevant results obtained by combining the attractive advantages of electrochemical transduction strategies (in terms of relatively simple hardware, versatility, interface with automatic logging and feasibility of application outside the laboratory environment) with those from biosensors technology. Important examples of enzyme electrochemical biosensors are those dedicated to the determination of glucose, alcohol or cholesterol are important examples. In addition, other types of different electrochemical biosensing approaches have emerged strongly in the last years. Among these, the strategies involving affinity interactions have been shown to possess a large number of applications. Therefore, electrochemical immunosensors and DNA-based biosensors have been widely used to determine major and minor components in foodstuffs, providing sufficient data to evaluate food freshness, the quality of raw materials, or the origin of samples, as well as to determine a variety of compounds at trace levels related to food safety such as micotoxins, allergens, drugs residues or pathogen microorganisms. This review discusses some critical examples of the latest advances in this area, pointing out relevant methodologies related to the measurement techniques, including the use of nanostructured electrodes and strategies for signal amplification.

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A highly sensitive impedimetric label free immunosensor for Ochratoxin measurement in cocoa beans

Cited by 54 publications

References 18 publications

Impedimetric Label-Free Immunosensor on Disposable Modified Screen-Printed Electrodes for Ochratoxin A

Impedimetric Label-Free Immunosensor on Disposable Modified Screen-Printed Electrodes for Ochratoxin A

Developments in mycotoxin analysis: an update for 2017-2018

Electrochemical Affinity Biosensors in Food Safety

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