A highly sensitive ratiometric fluorescent probe for imaging endogenous hydrogen sulfide in cells

Abstract

Hydrogen sulfide has been shown to play important regulatory roles in more and more physiological and pathological processes. Therefore, it is necessary to develop highly sensitive, selective and reliable probes...

Analytical Methods for Detection of Gasotransmitter Hydrogen Sulfide Released from Live Cells

“…Compared with “turn-on” fluorescent probes, ratiometric fluorescent probes have been proposed to be more accurate for detecting H 2 S, independently of variables in quantitative analysis including variations of excitation intensity, environmental factors, light scattering, and concentration of probe [ 55 ]. An et al [ 56 ] reported the quinoline quaternary ammonium salt derivative-based ratiometric fluorescent probe (referred to as QL-N 3 ). The QL-N 3 probe exhibited two fluorescence emission peaks at 525 and 605 nm with different excitation wavelengths of 385 and 521 nm, and the ratio between fluorescence intensities of two peaks was positively related with the H 2 S concentration.…”

A near‐infrared fluorescent probe for detecting hydrazine metabolized from isoniazid in living cells

“…In recent years, many fluorescent probes have been reported for detecting N 2 H 4 in living cells [13][14][15][16][17][18][19][20][21][22]. Near-infrared fluorescent probes (650-900 nm) are particularly suitable for detecting small molecules in vivo, because they cause little damage to tissue and have good tissue penetration [24,[26][27][28][29][30][31][32][33][34][35][36].…”

“…Near‐infrared fluorescent probes (650–900 nm) are particularly suitable for detecting small molecules in vivo , because they cause little damage to tissue and have good tissue penetration [24, 26–36]. Dicyanoisophorone analogues have been widely used in near‐infrared fluorescent probe design because of their simple synthesis and large Stokes’ shift [27, 37–40].…”

In Situ Detection of Hydrogen Sulfide in 3D-Cultured, Live Prostate Cancer Cells Using a Paper-Integrated Analytical Device

“…Several studies investigating non-destructive methods to reliably detect free H 2 S gas in living cells have been reported [16][17][18]. In particular, paper-based colorimetric assays utilizing silver/Nafion™/polyvinylpyrrolidone (PVP) membranes are able to detect quantitatively endogenous H 2 S in live cancer cells without the need for expensive and large instruments or special probes for H 2 S [19].…”