A highly specific antibody response after protein prime-peptide boost immunization with Eppin/B-cell epitope in mice

Chen, Zhengqiong; Wang, He; Wu, Yuzhang; Yan, Ping; He, Hongchao; Ji, Zhang; Yang, Xia; Shen, Zhaohua; Liang, Zhiqing; Li, Jintao

doi:10.4161/hv.7.8.16279

Cited by 4 publications

(5 citation statements)

References 24 publications

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“…With regard to the anti-fertility mechanism of Eppin, the results presented here were in agreement with our previous findings 3,27 and another report. 30 Anti-eppin antibody could leads to a reduction of fertility mainly by interfering with the function of sperm, but not the function of the testis.…”

Section: Discussionsupporting

confidence: 93%

“…In this study, pregnancy rate dropped to about 31.7%, which was similar to that seen in other researches. 3,27 This would suggest that there must be some difficulties in completely inhibiting the rodent sperm motility.…”

Section: Discussionmentioning

confidence: 99%

“…27 Briefly, the cauda epididymidis was separated, trimmed free of fat, and placed in 200 μL PBS containing 0.1% BSA. A 1 mL syringe was used to suck the PBS and flush the epididymis lumens thrice, and then express the residue fluid in the epidydimal lumens.…”

Section: Detection Of Iga In Epididymis Lavages Of Immunized Micementioning

confidence: 99%

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Nasal immunization using a mimovirus vaccine based on the Eppin B-cell epitope induced suppressed fertility in mice

Chen

Shen

et al. 2014

Human Vaccines & Immunotherapeutics

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

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Nasal immunization using a mimovirus vaccine based on the Eppin B-cell epitope induced suppressed fertility in mice

Chen

Shen

et al. 2014

Human Vaccines & Immunotherapeutics

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“…Similar to previous studies, this purity may satisfy the requirements of the contraceptive trial. 23,24 In our experiment, gradually increasing antibody titres were observed and peaked 12 weeks after the first peptide immunization. This observed antibody titre was similar to those seen in previous studies.…”

Section: Analysis Of Male Reproductive Organssupporting

confidence: 55%

“…Peptides were then synthesized, and the purity reached 90%. Similar to previous studies, this purity may satisfy the requirements of the contraceptive trial …”

Section: Discussionmentioning

confidence: 65%

ZNF185‐derived peptide induces fertility suppression in mice

Fan

Zhao

Pan

et al. 2018

Journal of Peptide Science

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Zinc finger protein 185 (ZNF185) belongs to the ZNF family and is involved in male reproduction. However, it is unclear whether ZNF185 may be a target candidate for contraceptive vaccines. In this study, antigenic peptides derived from ZNF185 were prepared, and their immune contraceptive effects were investigated using mice. Results from enzyme‐linked immunosorbent assay (ELISAs) showed that peptide immunization induced an antibody titre increase that reached a peak in week 12. Peptide‐3 and peptide‐4 were then chosen for subsequent experiments. The results of the fertility assays showed that peptide immunization inhibited the mating and fertility rates of the mice, whereas there were no obvious changes in the number of pups per litter. Subsequently, epididymal sperm was analysed. The results demonstrated that the sperm count and sperm motility were significantly decreased in the peptide group, while the amount of abnormal sperm was significantly increased in the peptide‐3 group. The male reproductive organs were also evaluated. There were no obvious differences in testis or epididymal weights, in the diameters of the seminiferous tubules, or in the thicknesses of the seminiferous epithelium between the peptide group and the phosphate buffer saline (PBS) group. In addition, histological analysis indicated that there were no obvious pathologic changes in testis and epididymal histology in the peptide group; however, the number of spermatozoa present in the epididymal lumen of the peptide group was significantly decreased when compared with the PBS group. Our study demonstrates for the first time that peptides derived from ZNF185 may induce fertility suppression in mice without damaging reproductive organs. These peptides have the potential to be used as a male contraceptive vaccine.

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Fertility Control in Wildlife: Review of Current Status, Including Novel and Future Technologies

Garside

Gebril

Alsaadi

et al. 2014

Advances in Experimental Medicine and Biology

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A highly specific antibody response after protein prime-peptide boost immunization with Eppin/B-cell epitope in mice

Cited by 4 publications

References 24 publications

Nasal immunization using a mimovirus vaccine based on the Eppin B-cell epitope induced suppressed fertility in mice

Nasal immunization using a mimovirus vaccine based on the Eppin B-cell epitope induced suppressed fertility in mice

ZNF185‐derived peptide induces fertility suppression in mice

Fertility Control in Wildlife: Review of Current Status, Including Novel and Future Technologies

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