A highly versatile adaptor protein for the tethering of growth factors to gelatin-based biomaterials

Addi, Cyril; Murschel, Frédéric; Liberelle, Benoît; Riahi, Nesrine; Crescenzo, Grégory De

doi:10.1016/j.actbio.2017.01.014

Cited by 15 publications

(19 citation statements)

References 56 publications

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“…A different concept to leverage on peptide affinities is the formation of coiled coil structures between two peptides with complementary coil domains. [152][153][154] This strategy implies modifying the target GF with one of the peptide tags (usually by recombinant technology), while immobilizing the other one at the biomaterial surface. Then, by incubating both together, the coil peptides will link with one another, functionalizing the biomaterial with the factor(s).…”

Section: Selective Ligands For Gf Capturementioning

confidence: 99%

“…Furthermore, correct tagging of the GF will allow maintenance of its bioactivity, since the surface immobilization occurs via noncovalent binding and does not affect the protein conformation nor the receptor-binding region. [152][153][154] This concept has been explored for several GFs and different regenerative applications. For example, tagged EGF and VEGF were both tethered to a chondroitin sulfate surface functionalized with complementary coil peptides, resulting in major increases in endothelial and smooth muscle cell survival after 2 days in serum-free medium.…”

Section: Selective Ligands For Gf Capturementioning

confidence: 99%

Section: Solid‐phase Presentationmentioning

confidence: 99%

“…This method preserved GF bioactivity, increasing smooth muscle cell survival and endothelial cell proliferation in vitro in reduced serum and serum‐free conditions. [ 153 ] In a different application of this concept, PDGF‐BB was combined with a PEG‐based scaffold to construct an in vitro model of the perivascular niche. In order to study the behavior of MSCs resident in this microenvironment, the authors constructed an engineered niche, analogous to its in vivo counterpart.…”

Section: Solid‐phase Presentationmentioning

confidence: 99%

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Biomaterials for Sequestration of Growth Factors and Modulation of Cell Behavior

Teixeira

Domingues

Shevchuk

et al. 2020

Adv Funct Materials

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Growth factors (GFs) are proteins secreted by cells that regulate a variety of biological processes. Although they have long been proposed as potent therapeutic agents, their administration in a soluble form has proven costly and ineffective due to their short half-lives in biological environments. Biomaterial-based approaches are increasingly sought as alternatives to improve the efficacy or, ideally, replace the need for exogenous administration of GFs in regenerative medicine strategies. The means by which these systems evolve from biomaterials for conventional controlled release of GFs to the recent extracellular matrix (ECM)-inspired approaches for sequestering these labile molecules and regulating their spatiotemporal activity and presentation are reviewed. Focus is placed on biomaterials functionalized either with ECM components, which show promiscuous GF binding, or with targeted GF ligands (antibodies, aptamers, or peptides). The potential of synthetic platforms with abiotic affinity as cost-effective alternatives to the current biological ligands is also discussed. Overall, the various GF sequestering systems developed so far have remarkably improved the activity of GFs at reduced doses and, in some cases, completely avoided the need for their exogenous administration to guide cell fates. These bioinspired concepts thus enable the rational exploration of the full therapeutic potential of GFs in regenerative medicine.

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Section: Selective Ligands For Gf Capturementioning

confidence: 99%

Section: Selective Ligands For Gf Capturementioning

confidence: 99%

Section: Solid‐phase Presentationmentioning

confidence: 99%

Section: Solid‐phase Presentationmentioning

confidence: 99%

See 2 more Smart Citations

Biomaterials for Sequestration of Growth Factors and Modulation of Cell Behavior

Teixeira

Domingues

Shevchuk

et al. 2020

Adv Funct Materials

View full text Add to dashboard Cite

show abstract

“…Similarly, the fibronectin-derived collagen binding domain (CBD) as well as coiled-coil domains were combined for the purpose of non-covalently immobilizing the growth factors, basic fibroblast growth factor (bFGF) and endothelial growth factor (EGF), to a hydrogel [229]. In this study, the CBD was genetically fused the C-terminal of the Kcoil coiled-coil, as well as bFGF and EGF, both with an Ecoil domain (Kcoil’s binding partner) (Fig.…”

Section: Targeting the Extracellular Space – Modules In Biomaterialsmentioning

confidence: 99%

Bottom-up approaches in synthetic biology and biomaterials for tissue engineering applications

Weisenberger

Deans

2018

Journal of Industrial Microbiology and Biotechnology

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Synthetic biologists use engineering principles to design and construct genetic circuits for programming cells with novel functions. A bottom-up approach is commonly used to design and construct genetic circuits by piecing together functional modules that are capable of reprogramming cells with novel behavior. While genetic circuits control cell operations through the tight regulation of gene expression, a diverse array of environmental factors within the extracellular space also has a significant impact on cell behavior. This extracellular space offers an addition route for synthetic biologists to apply their engineering principles to program cell-responsive modules within the extracellular space using biomaterials. In this review, we discuss how taking a bottom-up approach to build genetic circuits using DNA modules can be applied to biomaterials for controlling cell behavior from the extracellular milieu. We suggest that, by collectively controlling intrinsic and extrinsic signals in synthetic biology and biomaterials, tissue engineering outcomes can be improved.

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Bioinspired Amyloid Fibril‐Based Hydrogel with Engineering Programable Functionalities for Diverse Applications

Wang

Xiao

et al. 2023

Adv Funct Materials

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Natural proteins display organized hierarchical structures and tailored functionalities that cannot be achieved by synthetic approaches, highlighting the increased interest in developing protein‐based materials. Protein self‐assembly allows fabricating sophisticated supramolecular structures from relatively simple building blocks, a strategy naturally employed by amyloid proteins and intrinsically disordered proteins. However, the design of self‐assembled bioinspired materials with multi functionalities is still challenging. Inspired by the natural self‐assembly proteins (such as mussel foot proteins and amyloid proteins), a temperature‐inducible engineering programable hydrogel‐like amyloid nanostructure is developed by using a genetically modular fusion approach. The resulting hydrogel‐like assemblies display outstanding adhesive capacity, high stability, and broad substrate universality. The employed SpyCatcher/SpyTag system allows modifying the hydrogel‐like assemblies with any functional proteins of interest. Owing to their strong adhesive capacity and functional flexibility, such amyloid fibril‐based hydrogel shows advantages in the immobilization of diverse enzymes for highly efficient biocatalysis, fabrication of multi‐layered functional coatings, and construction of functionalized 3D scaffold for cell culture. Overall, a modular and straightforward approach is established to obtain a genetically programable nanostructure platform. The novel hydrogel‐like assemblies described here may be potentially applied to but not limited to synthetic biology, surface/interface engineering, and tissue engineering.

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A highly versatile adaptor protein for the tethering of growth factors to gelatin-based biomaterials

Cited by 15 publications

References 56 publications

Biomaterials for Sequestration of Growth Factors and Modulation of Cell Behavior

Biomaterials for Sequestration of Growth Factors and Modulation of Cell Behavior

Bottom-up approaches in synthetic biology and biomaterials for tissue engineering applications

Bioinspired Amyloid Fibril‐Based Hydrogel with Engineering Programable Functionalities for Diverse Applications

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