A Histomorphological Pattern Analysis of Pulmonary Tuberculosis in Lung Autopsy and Surgically Resected Specimens

Gupta, Mamta; Lobo, Flora D.; Adiga, Deepa; Gupta, Abhishek

doi:10.1155/2016/8132741

Cited by 19 publications

(16 citation statements)

References 20 publications

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“…During the progression of TB, the infiltration of inflammatory mononuclear cells leads to the development of granulomas; a habitable niche for Mtb [69]. Furthermore, acute bronchopneumonia and necrotizing granulomas have been correlated with the pathology of pulmonary TB [70]. Remarkably, we observed a decrease in the Mtb burden and disease pathology of the lungs of the C6 administered group and augmented the potency of BCG (Fig.…”

Section: Discussionsupporting

confidence: 51%

A multiple T cell epitope comprising DNA vaccine boosts the protective efficacy of Bacillus Calmette–Guérin (BCG) against Mycobacterium tuberculosis

et al. 2020

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Background Approximately 80% - 90% of individuals infected with latent Mycobacterium tuberculosis (Mtb) remain protected throughout their life-span. The release of unique, latent-phase antigens are known to have a protective role in the immune response against Mtb. Although the BCG vaccine has been administered for nine decades to provide immunity against Mtb, the number of TB cases continues to rise, thereby raising doubts on BCG vaccine efficacy. The shortcomings of BCG have been associated with inadequate processing and presentation of its antigens, an inability to optimally activate T cells against Mtb, and generation of regulatory T cells. Furthermore, BCG vaccination lacks the ability to eliminate latent Mtb infection. With these facts in mind, we selected six immunodominant CD4 and CD8 T cell epitopes of Mtb expressed during latent, acute, and chronic stages of infection and engineered a multi-epitope-based DNA vaccine (C6). Result BALB/c mice vaccinated with the C6 construct along with a BCG vaccine exhibited an expansion of both CD4 and CD8 T cell memory populations and augmented IFN-γ and TNF-α cytokine release. Furthermore, enhancement of dendritic cell and macrophage activation was noted. Consequently, illustrating the elicitation of immunity that helps in the protection against Mtb infection; which was evident by a significant reduction in the Mtb burden in the lungs and spleen of C6 + BCG administered animals. Conclusion Overall, the results suggest that a C6 + BCG vaccination approach may serve as an effective vaccination strategy in future attempts to control TB.

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Section: Discussionsupporting

confidence: 51%

A multiple T cell epitope comprising DNA vaccine boosts the protective efficacy of Bacillus Calmette–Guérin (BCG) against Mycobacterium tuberculosis

et al. 2020

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“…Gupta et al studied 40 cases diagnosed as pulmonary tuberculosis with a mean age was 41 years with a male preponderance (92.5%) which was not consistent with the present study. [16] A study was done by Meel et al on 200 cases which showed granulomas with carbon and silica particles in 1% males and 7% females. [17] These nontuberculous (dust reticulation) granulomas were not observed in the present study.…”

Section: Granulomatous Diseasesmentioning

confidence: 99%

Histopathological Pattern of Lungs on Post-Mortem Specimen – A Study of 100 Cases

Kaur¹,

Gupta²,

Singh³

et al. 2017

Ann. Int. Med. Den. Res.

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Background: Lungs are a major organ involved in infectious, inflammatory, occupational and neoplastic diseases. Lungs are also involved secondarily in almost all terminal events. Autopsy is an important tool to identify cause and manner of death and hence to establish preventive methods. Aims: To study the histopathological pattern of lungs in 100 post mortem cases. Methods: This was an observational study on 100 post-mortem specimens of lungs received in the department of Pathology, GMC, Patiala for a period of 1 year from August 2014 onwards. A thorough gross examination was done on the received lung specimens and sections were taken from the representative areas. These sections were processed for paraffin blocks; slides were prepared and stained with routine Hematoxylin and Eosin stains. The slides were studied under the microscope and the results were noted. Results: Out of the 100 cases, 63 showed terminal nonspecific changes; 19 cases showed the evidence of chronic venous congestion. 10 cases were of pneumonia, 6 of granulomatous pathology, and 1 case of metastatic deposits of adenocarcinoma. Conclusion: Infections are the most commonly noticed primary lung pathology mainly comprising of pneumonia followed by tuberculosis. Hence, efforts should be made for the prevention of these infectious diseases.

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“…In this situation, granulomatous lesions suggestive of TB can be found in up to 60% of cases [ 17 ]. Unfortunately, an important number of patients with miliary TB will not be diagnosed before a patient’s death, and it will be confirmed during autopsy [ 18 , 19 , 20 , 21 ].…”

Section: Discussionmentioning

confidence: 99%

Miliary Tuberculosis Presenting with Hyponatremia and ARDS in an 82-Year-Old Immunocompetent Female

et al. 2018

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An immunocompetent 82-year-old female was admitted to our hospital due to fever without clear origin and hyponatremia. In the following days, an acute and bilateral pulmonary infiltrate appeared with a progressive worsening in respiratory function. Chest x-ray and CT (Computed tomography) showed bilateral reticulonodular infiltrates. Bronchoscopic aspiration and bronchoalveolar lavage (BAL), and transbronchial lung biopsy (TBBX) studies did not reveal microbiological and histopathological diagnosis. Broad-spectrum antibiotics were non-effective, and the patient died due to respiratory failure. Necropsy study revealed a miliary tuberculosis affecting lungs, liver, bone marrow, spleen, kidney, arteries, pancreas, and adrenal glands. Some weeks after the patient´s death, mycobacterial cultures from sputum, BAL and TBBX samples were positive for Mycobacterium tuberculosis.

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A Histomorphological Pattern Analysis of Pulmonary Tuberculosis in Lung Autopsy and Surgically Resected Specimens

Cited by 19 publications

References 20 publications

A multiple T cell epitope comprising DNA vaccine boosts the protective efficacy of Bacillus Calmette–Guérin (BCG) against Mycobacterium tuberculosis

A multiple T cell epitope comprising DNA vaccine boosts the protective efficacy of Bacillus Calmette–Guérin (BCG) against Mycobacterium tuberculosis

Histopathological Pattern of Lungs on Post-Mortem Specimen – A Study of 100 Cases

Miliary Tuberculosis Presenting with Hyponatremia and ARDS in an 82-Year-Old Immunocompetent Female

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