A homogenous electrochemical sensing DNA sensor by using bare Au electrode based on potential-assisted chemisorption technique

Kong, Dewen; Liao, Fang; Lin, Yan; Cheng, Lin; Peng, Hong Mei; Zhang, Jing; Cui, Hanfeng; Hong, Nian; Chen, ChaDan; Wei, Guobing; Fan, Hao

doi:10.1016/j.snb.2018.03.011

Cited by 12 publications

(4 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Electrochemical genosensing technology for DNA diagnostics has been recently developed [ 68 , 83 ]. Of the DNA sensing methods, electrochemical detection has the advantages of inexpensive equipment, sensitivity, and rapid performance.…”

Section: Electrochemical Detection Of Biomoleculesmentioning

confidence: 99%

Recent Advances in Electrochemical Sensors for the Detection of Biomolecules and Whole Cells

2020

View full text Add to dashboard Cite

Electrochemical sensors are considered an auspicious tool to detect biomolecules (e.g., DNA, proteins, and lipids), which are valuable sources for the early diagnosis of diseases and disorders. Advances in electrochemical sensing platforms have enabled the development of a new type of biosensor, enabling label-free, non-destructive detection of viability, function, and the genetic signature of whole cells. Numerous studies have attempted to enhance both the sensitivity and selectivity of electrochemical sensors, which are the most critical parameters for assessing sensor performance. Various nanomaterials, including metal nanoparticles, carbon nanotubes, graphene and its derivatives, and metal oxide nanoparticles, have been used to improve the electrical conductivity and electrocatalytic properties of working electrodes, increasing sensor sensitivity. Further modifications have been implemented to advance sensor platform selectivity and biocompatibility using biomaterials such as antibodies, aptamers, extracellular matrix (ECM) proteins, and peptide composites. This paper summarizes recent electrochemical sensors designed to detect target biomolecules and animal cells (cancer cells and stem cells). We hope that this review will inspire researchers to increase their efforts to accelerate biosensor progress—enabling a prosperous future in regenerative medicine and the biomedical industry.

show abstract

Section: Electrochemical Detection Of Biomoleculesmentioning

confidence: 99%

Recent Advances in Electrochemical Sensors for the Detection of Biomolecules and Whole Cells

2020

View full text Add to dashboard Cite

show abstract

“…Examples of sensitive DNA detection systems involving this approach include detection of plant pathogen DNA using isothermal amplification in combination with a gold nanoparticle reporter, detection of DNA sequences relevant to Mycobacterium Tuberculosis using a gold surface functionalized with thiol modified DNA sequences, and E.coli DNA detection using a graphene oxide-chitosan composite decorated with nickel ferrite nanoparticles to achieve 10 -16 M sensitivity (Gaffar et al, 2017;Lau and Botella, 2017;Tiwari et al, 2015). DPV has been used to achieve 2.3 pM sensitivity for DNA hybridization when a methylene blue tagged hairpin probe DNA film was immobilized using assisted potential deposition and in similar work a LoD of 3.4 pM was discovered for a ferrocene tagged DNA probe deployed in a similar configuration (Hong et al, 2017;Kong et al, 2018). Finally, simultaneous detection of Legionella and Legionella pneumophila was achieved by using a signal-off double DNA probe electrochemical sensor with ferrocene and methylene blue hairpin probes where signal changes arise through specific cleavage of restriction sites within the probe sequences .…”

Section: Differential Pulse Voltammetry (Dpv)mentioning

confidence: 99%

A review of microfabricated electrochemical biosensors for DNA detection

Blair

Corrigan

2019

Biosensors and Bioelectronics

130

View full text Add to dashboard Cite

This review article presents an overview of recent work on electrochemical biosensors developed using microfabrication processes, particularly sensors used to achieve sensitive and specific detection of DNA sequences. Such devices are important as they lend themselves to miniaturisation, reproducible mass-manufacture, and integration with other previously existing technologies and production methods. The review describes the current state of these biosensors, novel methods used to produce them or enhance their sensing properties, and pathways to deployment of a complete point-ofcare biosensing system in a clinical setting.

show abstract

“…In terms of the electrode material and design for the microchip application, Kong et al [13] reported that gold (Au) was used as the electrode and had a high sensitivity without contamination. Robinson et al [14] reported that the Cu and Pt could be used as an electrode with a 'point contact' ball shape, which could reduce the contamination and detect the gas with the Cu of high activation enthalpy in the reaction.…”

Section: Introductionmentioning

confidence: 99%

“…Robinson et al [14] reported that the Cu and Pt could be used as an electrode with a 'point contact' ball shape, which could reduce the contamination and detect the gas with the Cu of high activation enthalpy in the reaction. Yong et al [15] also used the inert Pt for contact detection for the reaction of H 2 S. The Au [13,16] and Pt [10. 14, 15] are noble and expensive metal materials.…”

Section: Introductionmentioning

confidence: 99%

Design and fabrication of a Ti/Al thin-film electrode in the meander-shaped microchannel and its application for promoting capillary-driven dielectrophoresis blood separation

Lai

Yeh

Chung³

2019

J. Micromech. Microeng.

View full text Add to dashboard Cite

In this article, a Ti/Al thin film with Ti as an adhesive layer that merits high conductivity, excellent adhesion and low cost is used as the electrode in a biomedical blood separation chip instead of the conventional biomedical electrode mostly made of expensive Au and/or Pt. The design and fabrication of the meander-shaped Ti/Al thin-film electrode are studied for the application to promote the capillary-driven dielectrophoresis (DEP) blood separation chip. The meandered electrode with a castellated profile of 0.6 and 0.2 mm spacing instead of the flat one is designed on the glass substrate to produce an asymmetric electric field for the DEP force to separate the blood. The meander-with-castellated electrode shape and gap affects the electric field gradient on the separation performance. The patterning of the electrode was performed by the low-cost CO2 laser-ablated polymer tape rather than an expensive-complex photolithography process and then followed by sputtering together with lift-off process. The Ti/Al thin film is coated on the channel backside rather than inside for contactless DEP to avoid the contact pollution of the sample as well to reduce the operation voltage. The COMSOL Multiphysics® simulation of the electric field and the blood separation test were done for verifying the design and experiment. In the simulation, the 0.2 mm castellated electrode can greatly enhance the asymmetric electric field peak of 24000 V m−1 compared to the flat electrode of about 12000 V m−1. Also, the 0.2 mm gap castellated electrode with three-time electric-field-gradient-pulse frequency can enhance the DEP force compared to the 0.6 mm gap electrode. In the experiment, the blood chip of 0.2 mm castellated electrode with the highly increased electric field and DEP force can shorten the starting separation time from 533 s to 253 s and enhance the separation efficiency from 20.0% to 51.7% at the applied voltage of 5 V and 1 MHz.

show abstract

A homogenous electrochemical sensing DNA sensor by using bare Au electrode based on potential-assisted chemisorption technique

Cited by 12 publications

References 28 publications

Recent Advances in Electrochemical Sensors for the Detection of Biomolecules and Whole Cells

Recent Advances in Electrochemical Sensors for the Detection of Biomolecules and Whole Cells

A review of microfabricated electrochemical biosensors for DNA detection

Design and fabrication of a Ti/Al thin-film electrode in the meander-shaped microchannel and its application for promoting capillary-driven dielectrophoresis blood separation

Contact Info

Product

Resources

About