A Hop Extract Lifenol® Improves Postmenopausal Overweight, Osteoporosis, and Hot Flash in Ovariectomized Rats

Abstract

Objective In order to assess the effectiveness of a hop extract (HE) for postmenopausal symptoms, the effects of Lifenol on ovariectomy-induced osteoporosis, hyperlipidemia, body weight increase, and hot flash were investigated in rats. Methods Female Sprague-Dawley rats were ovariectomized and subjected to a daily scheduled exercise training (15 min at 15 m/min) or treated with HE (30 or 100 mg/kg, oral) or 17β-estradiol (100 μg/kg, intraperitoneal) for 12 weeks. Body and visceral fat weights, serum lipid profiles, osteoporotic parameters in serum, and femoral bones were analyzed. Separately, forced running-induced dermal and rectal temperatures and blood flow velocity were measured in ovariectomized rats. Results Ovariectomy increased blood lipids including triglycerides, total cholesterol, and low-density lipoproteins, leading to visceral fat accumulation and overweight. Estrogen depletion caused osteoporosis, displaying decreased femoral bone weight, bone mineral density and content, and blood phosphorus level. The disturbances in lipid metabolism and bone resorption were recovered by treatment with HE in a dose-dependent manner. In addition, HE treatment shortened the duration of forced running-induced alterations in skin and rectal temperatures by reducing blood flow velocity. Conclusion The results indicate that HE attenuated overweight, osteoporosis, and hot flash in estrogen-deficient animals by regulating blood lipid profile and fat accumulation, blood estrogen and bone resorption factors, and dermal blood flow.

The Protective Effect of Xanthohumol on the Content of Selected Elements in the Bone Tissue for Exposed Japanese Quails to TCDD

“…The histomorphometric analysis of the lumbar vertebrae showed that trabecular bone volume was significantly higher in XN-treated mice. The results confirming the beneficial effects of prenylated flavonoids on bone resorption associated with osteoporosis were also obtained by Ban et al [ 55 ]. The use of Lifenol ® , which contains hops extracts, in ovariectomized rats significantly reduced the decrease in bone mineral density and mineral content.…”

Combination of Red Clover and Hops Extract Improved Menopause Symptoms in an Ovariectomized Rat Model

“…RHEC administration inhibited weight gain and abdominal fat accumulation, and this effect is similar to that seen after estrogen treatment in OVX rats [24]. is weight loss was not observed in the red clover alone [25]; weight loss was observed in high contents of xanthohumol in hops [26,27]. Biochanin A and xanthohumol contents in RHEC were approximately 1.09% and 0.033%, respectively.…”

“…In the RHEC group, an increase in TST was significantly suppressed under normal as well as under stress conditions. It was confirmed that TST decreased when 8-PN, one of the main components of hops, was administered to OVX rats [30]; moreover, it was found that changes in TST and rectal temperature decreased upon consumption of Lifenol ® [27], a standardized hops CO 2 extract. While the clinical test results of red clover for the iris have been abundantly reported [31], there are few Evidence-Based Complementary and Alternative Medicine reports about TST results in animals.…”

Sirt1/Foxo Axis Plays a Crucial Role in the Mechanisms of Therapeutic Effects of Erzhi Pill in Ovariectomized Rats

“…Treatments promoting the bone formation or inhibiting the bone resorption can achieve amelioration of PMOP, while estrogen deficiency may induce deterioration of PMOP. The ovariectomized rat model can simulate the pathological process of bone loss during the postmenopause stage in humans, and it has been widely used to evaluate the effects of drugs in PMOP [ 13 , 14 ]. We employed bilateral ovariectomy to establish the PMOP animal model, and the results are in accordance with these previous conclusions.…”