A HPTLC method for the identification of ferulic acid from Lycopodium clavatum

Srivastava, Sharad; Singh, Adarsh Pratap; Rawat, Ajay Kumar Singh

doi:10.1016/s2221-1691(12)60121-x

Cited by 9 publications

(6 citation statements)

References 16 publications

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“…The HPTLC method was simple, reproducible, and sensitive in the separation and determination of ferulic acid. It was used to estimate ferulic acid in Lycopodium clavatum [74], Setaria italica [75], and Ricinus communis Linn. [76].…”

Section: Hptlc Analysismentioning

confidence: 99%

Genetic Stability, Phenolic, Flavonoid, Ferulic Acid Contents, and Antioxidant Activity of Micropropagated Lycium schweinfurthii Plants

Mamdouh

Mahgoub

Gabr

et al. 2021

Plants

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Lycium schweinfurthii is a Mediterranean wild shrub rich in plant secondary metabolites. In vitro propagation of this plant may support the production of valuable dietary supplements for humanity, introduction of it to the world market, and opportunities for further studies. The presented study aimed to introduce an efficient and reproducible protocol for in vitro micropropagation of L. schweinfurthii and assess the genetic stability of micropropagated plants (MiPs) as well as to estimate phenolic, flavonoid, ferulic acid contents, and the antioxidant activity in leaves of micropropagated plants. Two DNA-based techniques, random amplified polymorphic DNA (RAPD) and inter-simple sequence repeats (ISSR), and one biochemical technique, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), were used to assess the genetic stability in MiPs. Spectrophotometric analysis was performed to estimate total phenolic and flavonoid contents and antioxidant activity of MiPs leaves, while ferulic acid content was estimated using high-performance thin-layer chromatography (HPTLC). Sufficient shoot proliferation was achieved at MS (Murashige and Skoog) medium supplemented with 0.4 mg L−1 kinetin and rooted successfully on half-strength MS medium fortified with 0.4 mg L−1 Indole-3-butyric acid (IBA). The Jaccard’s similarity coefficients detected in MiPs reached 52%, 55%, and 82% in the RAPD, ISSR, and SDS-PAGE analyses, respectively. In the dried leaves of MiPs, the phenolic, flavonoid, and ferulic acid contents of 11.53 mg gallic acid equivalent, 12.99 mg catechin equivalent, and 45.52 mg were estimated per gram, respectively. However, an IC50 of 0.43, and 1.99 mg mL−1 of MiP dried leaves’ methanolic extract was required to scavenge half of the DPPH, and ABTS free radicals, respectively. The study presented a successful protocol for in vitro propagation of a valued promising plant source of phenolic compounds.

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Section: Hptlc Analysismentioning

confidence: 99%

Genetic Stability, Phenolic, Flavonoid, Ferulic Acid Contents, and Antioxidant Activity of Micropropagated Lycium schweinfurthii Plants

Mamdouh

Mahgoub

Gabr

et al. 2021

Plants

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“…The TLC fingerprint of A. lanata was obtained according to the method of Srivastava et al (2012). A TLC chromatogram was carried out on an aluminum sheet of silica gel60 F 254 (E. Merck, Germany).…”

Section: Thin Layer Chromatographic (Tlc) Fingerprintmentioning

confidence: 99%

Evaluation of Antioxidant Activities, Total Phenolic and Total Flavonoid Contents of Aqueous Extracts of Leaf, Stem, and Root of Aerva lanata

Chewchinda¹,

Kongkiatpaiboon²,

Sithisarn³

2019

CMUJNS

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Aerva lanata (Amaranthaceae) is a tropical weed commonly found in fields and wasteland. Several biological activities of this plant have been reported, such as antihyperglycemic, antimicrobial, and anticancer activities. Different antioxidant assays including DPPH radical scavenging, ferric reducing antioxidant power (FRAP), and ABTS radical scavenging assays were assessed to compare antioxidant potentials of plant extracts. The total phenolic and flavonoid contents were determined. HPLC analysis was used to quantify the amount of ferulic acid. From the results, the leaf extract showed the strongest radical scavenging activity as measured by DPPH and ABTS assays with IC 50 values of 136 μg/ml and 58 mg TEAC/g extract, respectively. Similarly, the highest reducing power of the leaf extract was observed at 70 mmol FeSO 4 /100 g extract. HPLC quantification of ferulic acid yielded values of 1.58, 1.53, and 1.33 µg/100 g extract for the leaf, stem and root extracts, respectively. Thus, A. lanata leaf extract may be suitable for further development and application as pharmaceutical and nutraceutical products due to its potent in vitro antioxidant activities and high phenolic contents.

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“…FA has been quantified by many analytical methods, such as high-performance liquid chromatography (HPLC) coupled with photodiode array wavelength detector (PDA) or UV-Vis detector (Anselmi et al, 2006;Craparo et al, 2009;Kareparamban et al, 2013;Li et al, 2007;Li et al, 2004;Li, Bi, 2003;Lu et al, 2005;Nadal et al, 2015;Picone et al, 2009;Qi et al, 2007;Wang et al, 2011), liquid chromatography tandem mass spectrometry (Guy et al, 2009;Wang et al, 2013;Zhang et al, 2009), UV-Vis spectroscopy (Lima et al, 2013;Merlin et al, 2012), thin layer chromatography (Mabinya, Mafunga, Brand, 2006), high-performance thin layer chromatography (Hingse, Digole, Annapure, 2014;Srivastava, Singh, Singh Rawat, 2012), gas chromatography (Olthof et al, 2003), chemiluminescence (Shen et al, 2013), capillary electrophoresis (Lima, Duarte, Esteves, 2007), micellar electrokinetic chromatography (Guo et al, 2003), electrochemical analysis (Vilian, Chen, 2014) and paper-based platforms (Tee-Ngam et al, 2013). However, HPLC is considered the most reliable and popular methodology for investigating phenolic acids (Barberousse et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

“…Moreover, most of these studies are about quantification of FA coupled with other phenolic compounds or other components extracted from plants (Andreasen et al, 2000;Guo et al, 2003;Sen et al, 1991;Srivastava, Singh, Singh Rawat, 2012;Vichapong et al, 2010;Waldron et al, 1996), or about the quantification of FA in rat plasma (Li, Bi, 2003;Qi et al, 2007;Rondini et al, 2004). There are few studies about the analysis of FA in products with pharmaceutical potential and among these are even rarer those with a documented validation study (Nadal et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

A stability-indicating HPLC-PDA method for the determination of ferulic acid in chitosan-coated poly(lactide-co-glycolide) nanoparticles

Lima

Khalil

Mainardes

2017

Braz. J. Pharm. Sci.

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The development and validation of a simple and efficient method for the quantification of ferulic acid in poly (D,L-lactide-co-glycolide) (PLGA) nanoparticles coated with chitosan (CS) by reverse phase high performance liquid chromatography coupled to photodiode array detection was described. For the chromatographic analysis, a reverse phase C-18 column was used, mobile phase consisting of acetonitrile and 0.5% acetic acid (37:63, v/v), isocratically eluted at a flow rate of 1 mL/min. Drug determination was performed at 320 nm. The method was validated in terms of the selectivity, linearity, precision, accuracy, robustness, limits of detection and quantification. The method was linear in the range of 10 to 100 μg/mL (r=0.999) and presented limit of detection and quantification of 102 ng/mL and 310 ng/mL, respectively. The method was precise (intra and inter-day) based on relative standard deviation values (less than 3.20%). The recovery was between 101.06 and 102.10%. Robustness was demonstrated considering change in mobile phase proportion. Specificity assay showed no interference from the components of nanoparticles or from the degradation products derived from acidic and oxidative conditions. The proposed method was suitable to be applied in determining the encapsulation efficiency of ferulic acid in PLGA-CS nanoparticles and can be employed as stability indicating one.Uniterms: Nanoparticles. Ferulic acid/encapsulation efficiency. Ferulic acid/quantification. Stability. High performance liquid chromatography/validation. PLGA-poly(lactide-co-glycolide)/nanoparticles. Chitosan.

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A HPTLC method for the identification of ferulic acid from Lycopodium clavatum

Cited by 9 publications

References 16 publications

Genetic Stability, Phenolic, Flavonoid, Ferulic Acid Contents, and Antioxidant Activity of Micropropagated Lycium schweinfurthii Plants

Genetic Stability, Phenolic, Flavonoid, Ferulic Acid Contents, and Antioxidant Activity of Micropropagated Lycium schweinfurthii Plants

Evaluation of Antioxidant Activities, Total Phenolic and Total Flavonoid Contents of Aqueous Extracts of Leaf, Stem, and Root of Aerva lanata

A stability-indicating HPLC-PDA method for the determination of ferulic acid in chitosan-coated poly(lactide-co-glycolide) nanoparticles

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