A human papillomavirus type 16 vaccine by oral delivery of L1 protein

Sasagawa, Toshiyuki; Tani, Mayuko; Basha, Walid; Rose, Robert C.; Tohda, Hideki; Giga-Hama, Yuko; Azar, Khadijeh K.; Yasuda, Hideyo; Sakai, Akemi; Inoue, Masakí

doi:10.1016/j.virusres.2005.02.001

Cited by 35 publications

(16 citation statements)

References 28 publications

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“…In rural Gambia with a spread of prevalent HR HPV types, where funding is short supply, this is impractical. However, vaccines currently in development include oral based prophylactic vaccines (Baud et al, 2004;Berg et al, 2005;Sasagawa et al, 2005), which will be easier to store and administer and thus economically viable.…”

Section: Discussionmentioning

confidence: 99%

Cervical human papillomavirus infection and squamous intraepithelial lesions in rural Gambia, West Africa: viral sequence analysis and epidemiology

et al. 2005

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The development of effective strategies against cervical cancer in Africa requires accurate type specific data on human papillomavirus (HPV) prevalence, including determination of DNA sequences in order to maximise local vaccine efficacy. We have investigated cervical HPV infection and squamous intraepithelial lesions (SIL) in an unselected cohort of 1061 women in a rural Gambian community. Squamous intraepithelial lesions was diagnosed using cytology and histology, HPV was typed by PCR-ELISA of DNA extracts, which were also DNA sequenced. The prevalence of cervical HPV infection was 13% and SIL were observed in 7% of subjects. Human papillomavirus-16 was most prevalent and most strongly associated with SIL. Also common were HPV-18, -33, -58 and, notably, -35. Human papillomavirus DNA sequencing revealed HPV-16 samples to be exclusively African type 1 (Af1). Subjects of the Wolof ethnic group had a lower prevalence of HPV infection while subjects aged 25 -44 years had a higher prevalence of cervical precancer than older or younger subjects. This first report of HPV prevalence in an unselected, unscreened rural population confirms high rates of SIL and HPV infection in West Africa. This study has implications for the vaccination of Gambian and other African populations in the prevention of cervical cancer.

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Section: Discussionmentioning

confidence: 99%

Cervical human papillomavirus infection and squamous intraepithelial lesions in rural Gambia, West Africa: viral sequence analysis and epidemiology

et al. 2005

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“…Due to the potential of Sz. pombe to express recombinant mammalian or viral proteins [8,[11][12][13], fission yeast was used as transport vehicle for proteinaceous antigens to APC resulting in antigen-specific T-cell responses [8,9]. Furthermore, Sz.…”

Section: Resultsmentioning

confidence: 99%

Schizosaccharomyces pombe: A novel transport vehicle of functional DNA and mRNA into mammalian antigen-presenting cells

Walch‐Rückheim

Breinig

2014

Vaccine

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“…To establish an edible HPV16 vaccine Sasagawa et al, (2005) constructed a recombinant HPV16 L1-expressing Schizosaccharomyces pombe yeast strain to be administrated as freezedried yeast powder orally as an edible vaccine, with or without the mucosal adjuvant heatlabile toxin LT (R192G), to mice. After the third immunization, none of the mice that received the edible HPV16 vaccine showed specific antibody responses, whereas all of the positive controls that were administered intranasally with 5 μg of HPV16-virus-like particles (VLP) had serum IgG, and genital IgA and IgG that reacted with HPV16-VLP in enzymelinked immunosorbent assays (ELISAs).…”

Section: Hpv L1 Antigens As An Edible Vaccine?mentioning

confidence: 99%