A hyperthermic seizure unleashes a surge of spreading depolarizations in Scn1a-deficient mice

Concurrent functional ultrasound imaging with graphene-based DC-coupled electrophysiology as a platform to study slow brain signals and cerebral blood flow under control and pathophysiological brain states

“…Seizures have been reported to co-occur with spreading depolarisations, 8,21 but due to the prominence of ACcoupled electrophysiology used by the epilepsy community chemoconvulsant-induced seizures we report pre-seizure rCBV oscillations and coupling between infra-slow brain signals and blood flow at seizure onset. Consistent with other recent findings, we observed a decrease in blood flow to the brain in the seconds before transition to seizure, and an increase during the seizure.…”

“…Seizures have been reported to co-occur with spreading depolarisations, 8,21 but due to the prominence of ACcoupled electrophysiology used by the epilepsy community this is a largely overlooked phenomenon even though it has important clinical implications. 22,23 When seizures were associated with CSD, we observed larger and more sustained increases in rCBV post-seizure in cortical regions and delayed and prolonged decreased rCBV in subcortical regions including the hippocampus (Fig.…”

Determining the Spread: Potential Biomarkers and Treatment for Seizure-Induced-Spreading Depolarization in a Mouse Model of Genetic Epilepsy

“…Aiba and colleagues 7 examined the spontaneous incidence and related semiology of SD in adult Scn1a +/RX mice. Scn1a +/RX and WT littermates were monitored using DC-band chronic EEG and infrared video.…”