A label-free electrochemical immunosensor based on 11-mercaptoundecanoic acid grafted chitosan and poly(N-methylaniline) for the detection of carcinoembryonic antigen

Direksilp, Chatrawee; Parinyanitikul, Napa; Ariyasajjamongkol, Nuttha; Sirivat, Anuvat

doi:10.1016/j.bioelechem.2023.108446

Cited by 10 publications

(1 citation statement)

References 65 publications

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“…The limit of detection (LOD), calculated based on a signal-to-noise ratio of 3 (S/N = 3), is 6.3 pg mL −1 . A comparison between the determination of CEA using different methods is demonstrated in Table S2 ( Supplementary Materials ) [ 51 , 52 , 53 , 54 , 55 , 56 , 57 ]. The LOD was lower than that obtained using square wave voltammetry [ 51 ], chronoamperometric [ 52 ] or electrochemiluminescence [ 53 ] detection through sandwich immunoassay, or chemiluminescence determination based on aptamer functionalized magnetic silicon composite (Apt/AuNPs/PDDA-SiO 2 @Fe 3 O 4 ) [ 54 ], or EIS determination based on the immobilization of the Ab on the poly(ethyleneglycol)-NH 2 -connected, pyrenebutyric acid-functionalized, graphene/gold nanoparticle-modified GCE (BSA/Ab/AuNPs/PPYGR/GCE) [ 55 ], or an 11-mercaptoundecanoic-acid-grafted chitosan and poly(N-methylaniline)-modified screen-printed carbon electrode (BSA/Ab/tCHI/dPNMA/SPCE) [ 56 ], but higher than that of an aptasensor based on Fe-MOF-supported self-polymerized dopamine-decorated Au NPs (NH 2 -aptamer/Au@PDA@Fe-MOF/GCE) [ 57 ].…”

Section: Resultsmentioning

confidence: 99%

Sensitive Electrochemical Detection of Carcinoembryonic Antigen Based on Biofunctionalized Nanochannel Modified Carbonaceous Electrode

Zhou,

Wang,

et al. 2024

Molecules

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The convenient construction of carbon-based electrochemical immunosensors with high performance is highly desirable for the efficient detection of tumor biomarkers. In this work, an electrochemical immunosensor was fabricated by integrating a biofunctionalized mesoporous silica nanochannel film with a carbon-based electrode, which can enable the sensitive determination of carcinoembryonic antigen (CEA) in serum. The commonly used carbonaceous electrode, glassy carbon electrode (GCE), was employed as the supporting electrode and was pre-treated through electrochemical polarization to achieve the stable binding of a vertically ordered mesoporous silica film with amino groups (NH2-VMSF) without the use of any adhesive layer. To fabricate the immunorecognition interface, antibodies were covalently immobilized after the amino groups on the outer surface of NH2-VMSF was derivatized to aldehyde groups. The presence of amino sites within the high-density nanochannels of NH2-VMSF can facilitate the migration of negatively charged redox probes (Fe(CN)63-/4-) to the supporting electrode through electrostatic adsorption, leading to the generation of electrochemical signals. In the presence of CEA, the formation of immunocomplexes on the recognitive interface can reduce the electrochemical signal of Fe(CN)63-/4- on the supporting electrode. Based on this principle, the sensitive electrochemical detection of CEA was achieved. CEA can be determined to range from 0.01 ng mL−1 to 100 ng mL−1 with a limit of detection of 6.3 pg mL−1. The fabricated immunosensor exhibited high selectivity, and the detection of CEA in fetal bovine serum was achieved.

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Section: Resultsmentioning

confidence: 99%

Sensitive Electrochemical Detection of Carcinoembryonic Antigen Based on Biofunctionalized Nanochannel Modified Carbonaceous Electrode

Zhou,

Wang,

et al. 2024

Molecules

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Advances in Bioreceptor Layer Engineering in Nanomaterial‐based Sensing of Pseudomonas Aeruginosa and its Metabolites

Lapitan,

Felisilda,

Tiangco

et al. 2024

Chemistry An Asian Journal

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Pseudomonas aeruginosa is an opportunistic pathogen that infects wounds and burns and causes severe infections in humans. The high virulence, the emergence of antibiotic‐resistant strains, and the easy transmissibility of P. aeruginosa necessitate its fast detection and control. The gold standard for detecting P. aeruginosa, the plate culture method, though reliable, takes several days to complete. Therefore, developing accurate, rapid, and easy‐to‐use diagnostic tools for P. aeruginosa is highly desirable. Nanomaterial‐based biosensors are at the forefront of detecting P. aeruginosa and its secondary metabolites. This review summarises the biorecognition elements, biomarkers, immobilisation strategies, and current state‐of‐the‐art biosensors for P. aeruginosa. The review highlights the underlying principles of bioreceptor layer engineering and the design of nanomaterial‐based optical, electrochemical, mass‐based, and thermal biosensors. The advantages and disadvantages of these biosensors and their future point‐of‐care applications are also discussed.

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Cubic Na0.5Bi0.5TiO3 nanoperovskite significantly expands the application of sensitive immunosensor for the detection of carcinoembryonic antigen

Ouyang,

Feng,

Zhao

et al. 2024

Microchim Acta

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A label-free electrochemical immunosensor based on 11-mercaptoundecanoic acid grafted chitosan and poly(N-methylaniline) for the detection of carcinoembryonic antigen

Cited by 10 publications

References 65 publications

Sensitive Electrochemical Detection of Carcinoembryonic Antigen Based on Biofunctionalized Nanochannel Modified Carbonaceous Electrode

Sensitive Electrochemical Detection of Carcinoembryonic Antigen Based on Biofunctionalized Nanochannel Modified Carbonaceous Electrode

Advances in Bioreceptor Layer Engineering in Nanomaterial‐based Sensing of Pseudomonas Aeruginosa and its Metabolites

Cubic Na0.5Bi0.5TiO3 nanoperovskite significantly expands the application of sensitive immunosensor for the detection of carcinoembryonic antigen

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