A label-free fiber optic biosensor for Salmonella Typhimurium detection

Kaushik, Siddharth; Pandey, Amit; Tiwari, Umesh; Sinha, R. K.

doi:10.1016/j.yofte.2018.09.012

Cited by 32 publications

(21 citation statements)

References 38 publications

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“…However, the analysis time of the proposed immunosensor was six times lower compared to that of the Ω-shaped fiber-optic localized SPR sensor. In addition, a fiber-optic immunosensor with an LOD of 247 CFU/mL could detect S. typhimurium in milk with assay time similar to that of the developed WLRS sensor [ 52 ], while an optical grating based sensor provided a 4-time higher LOD for an assay duration of 10 min [ 53 ]. An immunosensor based on Hartman interferometry capable to detect S. typhimurium in 10 min has been reported in the literature, with an LOD 30 times higher than that provided by the proposed immunosensor [ 36 ].…”

Section: Resultsmentioning

confidence: 99%

Rapid Detection of Salmonella typhimurium in Drinking Water by a White Light Reflectance Spectroscopy Immunosensor

Angelopoulou

Tzialla

Voulgari³

et al. 2021

Sensors

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Biosensors represent an attractive approach for fast bacteria detection. Here, we present an optical biosensor for the detection of Salmonella typhimurium lipopolysaccharide (LPS) and Salmonella bacteria in drinking water, based on white light reflectance spectroscopy. The sensor chip consisted of a Si die with a thin SiO2 layer on top that was transformed into a biosensor through the immobilization of Salmonella LPS. The optical setup included a reflection probe with seven 200 μm fibers, a visible and near-infrared light source, and a spectrometer. The six fibers at the reflection probe circumference were coupled with the light source and illuminated the biosensor chip vertically, whereas the central fiber collected the reflected light and guided it to the spectrometer. A competitive immunoassay configuration was adopted for the analysis. Accordingly, a mixture of LPS or bacteria solution, pre-incubated for 15 min, with an anti-Salmonella LPS antibody was pumped over the chip followed by biotinylated secondary antibody and streptavidin for signal enhancement. The binding of the free anti-Salmonella antibody to chip-immobilized LPS led to a shift of the reflectance spectrum that was inversely related to the analyte concentration (LPS or bacteria) in the calibrators or samples. The total assay duration was 15 min, and the detection limits achieved were 4 ng/mL for LPS and 320 CFU/mL for bacteria. Taking into account the low detection limits, the short analysis time, and the small size of the chip and instrumentation employed, the proposed immunosensor could find wide application for bacteria detection in drinking water.

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Section: Resultsmentioning

confidence: 99%

Rapid Detection of Salmonella typhimurium in Drinking Water by a White Light Reflectance Spectroscopy Immunosensor

Angelopoulou

Tzialla

Voulgari³

et al. 2021

Sensors

View full text Add to dashboard Cite

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“…In 2020, Luo et al [119] presented an immunosensor based on dispersion turning point LPG incorporating GO to detect H5N1 virus, a common type of avian influenza virus. GO was deposited Regarding Salmonella typhimurium detection, in 2018, Kaushik et al [118] reported a single mode-tapered multimode-single mode (SMS) type of immunosensor. The interaction between the propagating modes of guided light and ambient medium increases due to the tapering of the single-mode.…”

Section: Virusmentioning

confidence: 99%

“…Over the years, optical fiber immunosensors have emerged as a promising alternative to traditional methods [ 117 , 118 , 119 ] for the detection of various analytes, including microorganisms, medical biomarkers and environmental pollutants. Moreover, in the specific case of optical fibers as biosensors (e.g., signal response due to RI changes), the molecular size of the analyte could have an influence on the biosensor’s performance, namely in the sensitivity and LOD.…”

Section: Biosensing Applicationsmentioning

confidence: 99%

Immunosensing Based on Optical Fiber Technology: Recent Advances

et al. 2021

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The evolution of optical fiber technology has revolutionized a variety of fields, from optical transmission to environmental monitoring and biomedicine, given their unique properties and versatility. For biosensing purposes, the light guided in the fiber core is exposed to the surrounding media where the analytes of interest are detected by different techniques, according to the optical fiber configuration and biofunctionalization strategy employed. These configurations differ in manufacturing complexity, cost and overall performance. The biofunctionalization strategies can be carried out directly on bare fibers or on coated fibers. The former relies on interactions between the evanescent wave (EW) of the fiber and the analyte of interest, whereas the latter can comprise plasmonic methods such as surface plasmon resonance (SPR) and localized SPR (LSPR), both originating from the interaction between light and metal surface electrons. This review presents the basics of optical fiber immunosensors for a broad audience as well as the more recent research trends on the topic. Several optical fiber configurations used for biosensing applications are highlighted, namely uncladded, U-shape, D-shape, tapered, end-face reflected, fiber gratings and special optical fibers, alongside practical application examples. Furthermore, EW, SPR, LSPR and biofunctionalization strategies, as well as the most recent advances and applications of immunosensors, are also covered. Finally, the main challenges and an outlook over the future direction of the field is presented.

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“…Formerly, there were explicit antigen predicaments to Ab1 location, as well as the considered inferior antibody (Ab2) predicaments to the antigen For effective expansion of electrochemical immunosensors, a restrained quantity of biomolecules on the electrode exterior is vital that frequently controls compassion of immunoassay . Consequently, discovering an innovative suggestion tag that is created on electrochemical opinion would be valued.…”

Section: Sensors and Biosensorsmentioning

confidence: 99%

Electrochemical Sensors and Biosensors Based on Graphene Functionalized with Metal Oxide Nanostructures for Healthcare Applications

et al. 2019

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Graphene has attracted wide consideration in recent years to the assembly of sensitive sensors and biosensors due to its unique and remarkable physical and electrochemical properties. Moreover, graphene, as an essential two‐dimensional carbon material with remarkably high quartz and electronic superiority, has also received significant research attention. This review presents the different synthesis techniques of graphene; graphene functionalized based electrochemical sensors and biosensors for various health care appellations. Further, were discussed on the basis of enhanced catalytic activity, improved detection limit in conjunction with sensitivity, and selectivity. Synergistic action of graphene and metal oxide nanostructure has contributed towards high activity as a biosensing material. The results with different sensors and biosensors for the detection of significant biomarkers such as protein sensor, electrochemical immune sensor, phytochrome sensor, cholesterol biosensor glucose, hydrogen peroxide, and nicotinamide adenine dinucleotide detection sensor etc., and highlighted the use of graphene and functionalized graphene in different sensing platforms. Finally, the challenges related to less aggregated graphene‐based electrochemical sensors and biosensors as well as future research directions are discussed.

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A label-free fiber optic biosensor for Salmonella Typhimurium detection

Cited by 32 publications

References 38 publications

Rapid Detection of Salmonella typhimurium in Drinking Water by a White Light Reflectance Spectroscopy Immunosensor

Rapid Detection of Salmonella typhimurium in Drinking Water by a White Light Reflectance Spectroscopy Immunosensor

Immunosensing Based on Optical Fiber Technology: Recent Advances

Electrochemical Sensors and Biosensors Based on Graphene Functionalized with Metal Oxide Nanostructures for Healthcare Applications

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