A label-free fluorescent sensor based on silicon quantum dots–MnO<sub>2</sub> nanosheets for the detection of α-glucosidase and its inhibitor

Liu, Jinying; Duan, Xinhe; Wang, Mengke; Su, Xingguang

doi:10.1039/c9an01680e

Cited by 30 publications

(16 citation statements)

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“…This is ascribed to that the sulfydryl in MTG is reductive and MnO 2 nanosheets can be decomposed by reductive substances. [36][37][38] The decomposition of MnO 2 nanosheets by MTG was further demonstrated by TEM ( Figure S1). It can be observed that the two-dimensional structure of MnO 2 nanosheets is destroyed after the addition of MTG.…”

Section: Decomposition Of Mno 2 Nanosheets By Mtgmentioning

confidence: 94%

“…MnO 2 nanomaterials can also be decomposed by reductive substances such as sulfydryl compounds, H 2 O 2 and ascorbic acid into Mn 2 + , resulting in a color change from brown to colorless. [36][37][38] Further considering the interaction of sulfhydryl with Hg 2 + , it is speculated that Hg 2 + can be colorimetrically detected based on the inhibited decomposition ability of sulfhydryl toward MnO 2 nanosheets.…”

Section: Introductionmentioning

confidence: 99%

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A Simple Colorimetric Probe for Sensitive Detection of Hg²⁺ Based on MnO₂ Nanosheets and Monothioglycerol

Ding

Zhao

et al. 2020

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It is of great significance to develop efficient platforms for the detection of hypertoxic Hg 2 +. Colorimetric probes have received much attention for the detection of Hg 2 + in the last decades. In this work, a colorimetric probe composed of MnO 2 nanosheets and monothioglycerol (MTG) was rationally designed for Hg 2 + detection, which was facially prepared via onestep reaction at room temperature. The formation of HgÀ S bond between Hg 2 + and MTG inhibited the decomposition ability of MTG toward MnO 2 nanosheets, thus resulting in a color change from colorless to brown. On this basis, the probe quantified Hg 2 + in the concentration range from 2 to 60 μM with a low detection limit of 0.75 μM. The probe was applied to the detection of Hg 2 + in tap water successfully, and was further fabricated into test strips for the convenience of use. Thereby, a simple and sensitive colorimetric probe for Hg 2 + is developed in this work, which holds future promise for monitoring Hg 2 + in the environment.

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Section: Decomposition Of Mno 2 Nanosheets By Mtgmentioning

confidence: 94%

Section: Introductionmentioning

confidence: 99%

A Simple Colorimetric Probe for Sensitive Detection of Hg²⁺ Based on MnO₂ Nanosheets and Monothioglycerol

Ding

Zhao

et al. 2020

ChemistrySelect

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“…According to previously reported studies, acarbose was commonly used as an anti-diabetic drug to precisely inhibit the catalytic activity of α-Glu. 9 Hence, the designed colorimetric and fluorometric assays of α-Glu activity have been performed to study the enzymatic inhibition ability using acarbose as a model. Once α-Glu was oppressed to acarbose, the catalytic hydrolysis of 4-HPαDG was refrained, which ended up by the decreased ability to generate HQ.…”

Section: Stability Of Simentioning

confidence: 99%

“…12,13 More particularly, due to their harmlessness and low toxicity, Si-based nanosensors could be used in human body as markers. 9,14 Thus, Si CNPs reveal an extensive dominance in biosensing and bioimaging. 15 Although an impressive progress for the preparation of Si CNPs has been achieved, however, the preparation procedures under tolerable parameters are still desirable.…”

Section: Introductionmentioning

confidence: 99%

α-Glucosidase-Triggered Reaction for Fluorometric and Colorimetric Assays Based on the Formation of Silicon-Containing Nanoparticles

et al. 2021

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Designing analytical approaches for enzymatic activity monitoring with high sensitivity and selectivity is of critical value for the diagnosis of diseases and biomedical studies. In this study, we have created a facile one-step synthetic route to prepare orange-red color and yellow fluorescent silicon-containing nanoparticles (Si CNPs) by mixing 3(2-aminoethylamino) propyl (dimethoxymethylsilane) and hydroquinone (HQ) in an aqueous solution. Inspired by the HQ-regulated facile synthetic step and the generation of HQ from α-glucosidase (α-Glu)-catalyzed hydrolysis of 4hydroxyphenyl-α-D-glucopyranosyl (4-HPαDG), we have designed a straightforward colorimetric and fluorometric α-Glu activity assay using a commercially available 4-HPαDG as the α-Glu substrate. Fluorescent and colorimetric assays for α-Glu activity measurement have been thereby established and exhibited detection limits as low as 0.0032 and 0.0046 U/mL, respectively. Under single excitation at 370 nm, the prepared Si CNPs emitted yellow fluorescence at 520 nm and exhibited an absorbance peak at 390 nm. In addition, the proposed approach reveals various advantages including easy operation, time-saving, and good anti-interference ability. Hence, it could improve the progress of fluorometric and colorimetric enzymatic activity assays with high sensitivity and simplicity. Moreover, the proposed approach was applied for α-Glu inhibitor screening, and its feasibility in real samples was measured by detecting the α-Glu activity in human serum samples.

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“…Electrochemical and oxidative properties of Mn oxides predestine them to be used as a component of nanocomposites, sensitive to a broad range of compounds. MnO 2 —based biosensors can detect, i.a., α-glucosidase, glucose, or glutathione (GSH) in human blood [ 19 , 20 , 21 ] or Salmonella typhimurium , a pathogenic bacterium, in food products [ 22 ].…”

Section: Introductionmentioning

confidence: 99%

Applications and Biological Activity of Nanoparticles of Manganese and Manganese Oxides in In Vitro and In Vivo Models

et al. 2021

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The expanding applications of nanotechnology seem to be a response to many technological, environmental, and medical challenges. The unique properties of nanoparticles allow for developing new technologies and therapies. Among many investigated compounds is manganese and its oxides, which in the form of nanoparticles, could be a promising alternative for gadolinium-based contrast agents used in diagnostic imaging. Manganese, which is essential for living organisms as an enzyme cofactor, under excessive exposure—for example, due to water contamination or as an occupational hazard for welders—can lead to neurological disorders, including manganism—a condition similar to Parkinson’s disease. This review attempts to summarise the available literature data on the potential applications of manganese and manganese oxide nanoparticles and their biological activity. Some of the published studies, both in vitro and in vivo, show negative effects of exposure to manganese, mainly on the nervous system, whereas other data suggest that it is possible to develop functionalised nanoparticles with negligible toxicity and novel promising properties.

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A label-free fluorescent sensor based on silicon quantum dots–MnO₂ nanosheets for the detection of α-glucosidase and its inhibitor

Abstract: We have successfully designed a fluorescence SiQD–MnO2 nanosheet sensing platform to detect α-glucosidase and acarbose for the first time.

Cited by 30 publications

References 30 publications

A Simple Colorimetric Probe for Sensitive Detection of Hg²⁺ Based on MnO₂ Nanosheets and Monothioglycerol

A Simple Colorimetric Probe for Sensitive Detection of Hg²⁺ Based on MnO₂ Nanosheets and Monothioglycerol

α-Glucosidase-Triggered Reaction for Fluorometric and Colorimetric Assays Based on the Formation of Silicon-Containing Nanoparticles

Applications and Biological Activity of Nanoparticles of Manganese and Manganese Oxides in In Vitro and In Vivo Models

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A label-free fluorescent sensor based on silicon quantum dots–MnO2 nanosheets for the detection of α-glucosidase and its inhibitor

Abstract: We have successfully designed a fluorescence SiQD–MnO2 nanosheet sensing platform to detect α-glucosidase and acarbose for the first time.

Cited by 30 publications

References 30 publications

A Simple Colorimetric Probe for Sensitive Detection of Hg2+ Based on MnO2 Nanosheets and Monothioglycerol

A Simple Colorimetric Probe for Sensitive Detection of Hg2+ Based on MnO2 Nanosheets and Monothioglycerol

α-Glucosidase-Triggered Reaction for Fluorometric and Colorimetric Assays Based on the Formation of Silicon-Containing Nanoparticles

Applications and Biological Activity of Nanoparticles of Manganese and Manganese Oxides in In Vitro and In Vivo Models

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Product

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A label-free fluorescent sensor based on silicon quantum dots–MnO₂ nanosheets for the detection of α-glucosidase and its inhibitor

A Simple Colorimetric Probe for Sensitive Detection of Hg²⁺ Based on MnO₂ Nanosheets and Monothioglycerol

A Simple Colorimetric Probe for Sensitive Detection of Hg²⁺ Based on MnO₂ Nanosheets and Monothioglycerol