A Label-Free Fluorescent Sensor Based on the Formation of Poly(thymine)-Templated Copper Nanoparticles for the Sensitive and Selective Detection of MicroRNA from Cancer Cells

Ma, Qian; Gao, Zhiqiang; Dayal, Hiranya; Li, Sam Fong Yau

doi:10.3390/chemosensors8030052

Cited by 7 publications

(3 citation statements)

References 50 publications

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“…let-7a was detected by an ssDNA padlock probe (Figure 11c). Once recognized, the resulting poly-T ssDNA was used as a template for the formation of fluorescent CuNPs, whose intense emission eliminated background signals showing a great degree of sensitivity (LOD of 70.6 fM) [89]. Magnetite (Fe 3 O 4 ) nanoparticles, associated with carbon (Fe 3 O 4 @C), were used as a quencher in a fluorescence-based detection of miR-20a.…”

Section: Other Metallic Nanoparticlesmentioning

confidence: 99%

microRNA Detection via Nanostructured Biochips for Early Cancer Diagnostics

Martino

Tammaro

Misso

et al. 2023

IJMS

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MicroRNA (miRNA) are constituted of approximately 22 nucleotides and play an important role in the regulation of many physiological functions and diseases. In the last 10 years, an increasing interest has been recorded in studying the expression profile of miRNAs in cancer. Real time-quantitative polymerase chain reaction (RT-qPCR), microarrays, and small RNA sequencing represent the gold standard techniques used in the last 30 years as detection methods. The advent of nanotechnology has allowed the fabrication of nanostructured biosensors which are widely exploited in the diagnostic field. Nanostructured biosensors offer many advantages: (i) their small size allows the construction of portable, wearable, and low-cost products; (ii) the large surface–volume ratio enables the loading of a great number of biorecognition elements (e.g., probes, receptors); and (iii) direct contact of the recognition element with the analyte increases the sensitivity and specificity inducing low limits of detection (LOD). In this review, the role of nanostructured biosensors in miRNA detection is explored, focusing on electrochemical and optical sensing. In particular, four types of nanomaterials (metallic nanoparticles, graphene oxide, quantum dots, and nanostructured polymers) are reported for both detection strategies with the aim to show their distinct properties and applications.

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Section: Other Metallic Nanoparticlesmentioning

confidence: 99%

microRNA Detection via Nanostructured Biochips for Early Cancer Diagnostics

Martino

Tammaro

Misso

et al. 2023

IJMS

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“…Compared to SNV detection, the simultaneous specific detection of miRNA homologous sequences is more challenging. Recently, researchers have tried to develop new SNV detection methods for simultaneous specific detection of miRNA homologous sequences. , For example, Ma et al reported a method for the specific detection of let-7a through ligase mediated rolling circle amplification (RCA). Generally, the identification of similar sequences was achieved by designing similar probes in multiple parallel detection systems.…”

Section: Introductionmentioning

confidence: 99%

Discrimination of Multiple Homologous Sequences Based on DNA Logic Gate and Elemental Labeling Technology

Kang,

Chen,

et al. 2024

Anal. Chem.

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The simultaneous discrimination of multiple homologous sequences faces challenges due to the high similarity of sequences and the complexity of the discrimination system in most reported works. Herein, a simple and ingenious analysis method was developed to identify eight miRNAs of the let-7 family by combining logic gates and entropy-driven catalytic (EDC)-based lanthanide labeling inductively coupled plasma mass spectrometry (ICP-MS) technology. Specifically, eight miRNAs were first divided into four types according to the difference of bases in the domains 2 and 3 on sequences. To identify the type of targets, a DNA logic gate was constructed with two strand displacement reactions on magnetic beads that could be initiated by different types of targets. Based on the difference of the output signals after two strand displacement reactions, the type of targets was distinguished preliminarily. Then, the discrimination of a specific target was achieved with EDC-based lanthanide labeling ICP-MS detection. By labeling the different magnetic probes with different elemental tags, a specific element signal released from magnetic beads after EDC could be detected by ICP-MS, and therefore, simultaneous detection of homologous sequences was completed. This work provided a novel and simple method for highly specific identification of homologous sequences with the assistance of a logic gate and can promote further development of elemental labeling ICP-MS in the field of multiple analysis.

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“…Fluorescence methods have attracted widespread attention due to their advantages of a fast response, high sensitivity and simple equipment [8][9][10]. The fluorescence material plays an important role in the sensitivity, selectivity and sensing speed of a fluorescence method [11,12]. There are some reports on the determination of TC by sensors based on fluorescence materials, such as carbon dots [13], Eu 3+ -functionalized silicon nanoparticles [14] and SYBR Green I [15].…”

Section: Introductionmentioning

confidence: 99%

Adamantane Three-Dimensional Porous Organic Framework as a Fluorescence Sensor for Rapid Determination of Tetracycline in Aquatic Products

et al. 2022

Chemosensors

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A fluorescence adamantane three-dimensional porous organic framework (AdaPOF) was synthesized via a Suzuki coupling reaction. The AdaPOF showed excellent fluorescence performance with a relative high quantum yield and fluorescence stability. Due to its excellent selectivity to tetracycline (TC), a fluorescence sensor based on AdaPOF was constructed for TC determination. The selective sensing mechanism of the AdaPOF towards TC was studied by density functional theory (DFT) calculation experiments. An AdaPOF–based fluorescence method for TC determination was established, with the linear range of 0.1–9.0 μmol/L (R2 = 0.9959) and the limit of detection (S/N = 3) of 43 nmol/L. Moreover, the fluorescence method was used to the determination of TC in aquatic products and the recoveries were ranged from 94.4% to 103.8%. The results obtained by this fluorescence method were consistent with those of the high-performance liquid chromatography (HPLC) method in the TC determination.

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A Label-Free Fluorescent Sensor Based on the Formation of Poly(thymine)-Templated Copper Nanoparticles for the Sensitive and Selective Detection of MicroRNA from Cancer Cells

Cited by 7 publications

References 50 publications

microRNA Detection via Nanostructured Biochips for Early Cancer Diagnostics

microRNA Detection via Nanostructured Biochips for Early Cancer Diagnostics

Discrimination of Multiple Homologous Sequences Based on DNA Logic Gate and Elemental Labeling Technology

Adamantane Three-Dimensional Porous Organic Framework as a Fluorescence Sensor for Rapid Determination of Tetracycline in Aquatic Products

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