A label free immunosensor for ultrasensitive detection of 17β-Estradiol in water

Singh, Aruna Chandra; Bacher, Gautam; Bhand, Sunil

doi:10.1016/j.electacta.2017.02.120

Cited by 31 publications

(13 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…When NPs were spiked in seawater samples, the obtained limit of detection was 23 μM, with an average recovery between 97.8 and 104.6%. Another example is the detection of 17-estradiol 77 in water samples using immunosensors with a good LOD of 0.0036 nM and a short analysis time of 10 minutes. Self-assembled monolayers of 11-mercaptoundecanoic acid were used to create such immunosensors by coupling the electrode with an anti-17-Estradiol antiserum primary monoclonal antibody specific to the antigen (17-estradiol) (MUA).…”

Section: Methods For Detection Of Emerging Contaminantsmentioning

confidence: 99%

Alternative methods of monitoring emerging contaminants in water: a review

Bhuvaneshwari

Nallathambi

Devasena

2022

Environ. Sci.: Processes Impacts

View full text Add to dashboard Cite

show abstract

Section: Methods For Detection Of Emerging Contaminantsmentioning

confidence: 99%

Alternative methods of monitoring emerging contaminants in water: a review

Bhuvaneshwari

Nallathambi

Devasena

2022

Environ. Sci.: Processes Impacts

View full text Add to dashboard Cite

show abstract

“…Testing for contaminants in water or dairy products such as milk or infant formula proves less restrictive. [256][257][258][259] An obstacle for POC electrochemical devices is the counter electrode (CE) and reference electrode (RE). Maintaining the RE and designing a suitable electrochemical cell can interfere with the portable use of devices.…”

Section: Emerging Technologymentioning

confidence: 99%

Electrochemical Impedance Spectroscopy (EIS) Based Label-Free Immunosensors

Robinson

Juska

O’Riordan

2022

Preprint

View full text Add to dashboard Cite

Electrochemical Impedance Spectroscopy (EIS) is a surface sensitive technique which examines the impedance of an electrochemical cell over a range of frequencies. By immobilising an antigen or antibody to the electrode surface, EIS shows potential for highly specific and sensitive immunosensor performance. Following an introduction into antibodies and EIS, this review presents immobilisation techniques for an immunosensor’s bioreceptor. It highlights electrode modifications such as self-assembled monolayers (SAMs), polymers and nanostructures for anchoring the bioreceptor to the surface. Stable immobilisation directly to these modifications is not always possible. Immobilisation techniques to these modified surfaces are explored including cross-linking and controlled orientation. EIS allows for label-free detection by monitoring the change in charge-transfer resistance or capacitance caused by changes in the electrode surface. Label-free immunosensors are the focus of this paper as they show potential for point-of-care (POC) devices.

show abstract

“…The bonds between antibodies and antigens are relatively weak and can be dissociated by changing the properties of the environments, i.e., pH and ionic strength. Singh et al developed a simple label-free immunosensor for 17β-estradiol [ 61 ]. Silver wire electrodes were modified with an 11-mercaptoundecanoic acid self-assembled monolayer (SAM) and the 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC)-N-hydroxy succinimide (NHS) chemistry was used to covalently bind the 17β-estradiol monoclonal antibodies.…”

Section: Eis Biosensors For the Detection Of Edsmentioning

confidence: 99%

Advances in Electrochemical Impedance Spectroscopy Detection of Endocrine Disruptors

Zamfir

Puiu

Bala

2020

Sensors

View full text Add to dashboard Cite

Endocrine disruptors (EDs) are contaminants that may mimic or interfere with the body’s hormones, hampering the normal functions of the endocrine system in humans and animals. These substances, either natural or man-made, are involved in development, breeding, and immunity, causing a wide range of diseases and disorders. The traditional detection methods such as enzyme linked immunosorbent assay (ELISA) and chromatography are still the golden techniques for EDs detection due to their high sensitivity, robustness, and accuracy. Nevertheless, they have the disadvantage of being expensive and time-consuming, requiring bulky equipment or skilled personnel. On the other hand, early stage detection of EDs on-the-field requires portable devices fulfilling the Affordable, Sensitive, Specific, User-friendly, Rapid and Robust, Equipment free, Deliverable to end users (ASSURED) norms. Electrochemical impedance spectroscopy (EIS)-based sensors can be easily implemented in fully automated, sample-to-answer devices by integrating electrodes in microfluidic chips. The latest achievements on EIS-based sensors are discussed and critically assessed.

show abstract

A label free immunosensor for ultrasensitive detection of 17β-Estradiol in water

Cited by 31 publications

References 26 publications

Alternative methods of monitoring emerging contaminants in water: a review

Alternative methods of monitoring emerging contaminants in water: a review

Electrochemical Impedance Spectroscopy (EIS) Based Label-Free Immunosensors

Advances in Electrochemical Impedance Spectroscopy Detection of Endocrine Disruptors

Contact Info

Product

Resources

About