A label-free impedimetric aptasensor for the detection of Bacillus anthracis spore simulant

Mazzaracchio, Vincenzo; Neagu, Daniela; Porchetta, Alessandro; Marcoccio, Eleonora; Pomponi, Alice; Faggioni, Giovanni; D’Amore, Nino; Notargiacomo, A.; Pea, Marialilia; Moscone, Danila; Palleschi, Giuseppe; Lista, Florigio; Arduini, Fabiana

doi:10.1016/j.bios.2018.11.017

Cited by 62 publications

(41 citation statements)

References 37 publications

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“…Inherent sensitivity, simplicity, and low cost of novel analytical methods, such as LAMP, electrochemical biosensors and Figure 4. Illustration of principle for B. cereus spore detection and electrochemical set-up for signal measurement using a miniaturized aptasensor (from Reference [97]).…”

Section: Discussionmentioning

confidence: 99%

“…In 2012, Bruno and Carrillo reported an aptameric sequence, which specifically binds to spores of B. anthracis and B. cereus [96]. Based on the binding properties of this sequence, a label-free impedimetric aptasensor has been developed recently (Figure 4) [97]. B. cereus spores were detected with a linear range between 10 4 CFU/mL and 5 × 10 6 CFU/mL, with a detection limit of 3 × 10 3 CFU/mL.…”

Section: Biosensorsmentioning

confidence: 99%

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Food Sensing: Detection of Bacillus cereus Spores in Dairy Products

Vidić

Chaix

Manzano³

et al. 2020

Biosensors

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Milk is a source of essential nutrients for infants and adults, and its production has increased worldwide over the past years. Despite developments in the dairy industry, premature spoilage of milk due to the contamination by Bacillus cereus continues to be a problem and causes considerable economic losses. B. cereus is ubiquitously present in nature and can contaminate milk through a variety of means from the farm to the processing plant, during transport or distribution. There is a need to detect and quantify spores directly in food samples, because B. cereus might be present in food only in the sporulated form. Traditional microbiological detection methods used in dairy industries to detect spores show limits of time (they are time consuming), efficiency and sensitivity. The low level of B. cereus spores in milk implies that highly sensitive detection methods should be applied for dairy products screening for spore contamination. This review describes the advantages and disadvantages of classical microbiological methods used to detect B. cereus spores in milk and milk products, related to novel methods based on molecular biology, biosensors and nanotechnology.

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Section: Discussionmentioning

confidence: 99%

Section: Biosensorsmentioning

confidence: 99%

Food Sensing: Detection of Bacillus cereus Spores in Dairy Products

Vidić

Chaix

Manzano³

et al. 2020

Biosensors

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“…Pandey et al showed an LDR between 1 to 10 5 parts per trillion. A similar strategy was used to detect B. anthracis spores [51].…”

Section: Aptasensor Electrochemistry Architecturesmentioning

confidence: 99%

Challenges in Electrochemical Aptasensors and Current Sensing Architectures Using Flat Gold Surfaces

Rozenblum

Pollitzer²,

Radrizzani

2019

Chemosensors

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In recent years, reagentless aptamer biosensors, named aptasensors, have shown significant advancements. Particularly, electrochemical aptasensors could change the field of biosensors in this era, where digitalization seems to be a common goal of many fields. Biomedical devices are integrating electronic technologies for detecting pathogens, biomolecules, small molecules, and ions, and the physical-chemical properties of nucleic acid aptamers makes them very interesting for these devices. Aptamers can be easily synthesized and functionalized with functional groups for immobilization and with redox chemical groups that allow for the conversion of molecular interactions into electrical signals. Furthermore, non-labeled aptamers have also been utilized. This review presents the current challenges involved in aptasensor architectures based on gold electrodes as transducers.

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“…The toxin was determined in milk and human serum across a 0.27-268 pg•mL −1 range, with an LOD of 0.11 pg•mL −1 [135]. Mazzaracchio et al [136] also prepared an impedimetric biosensor for Bacillus anthracis spore simulant (B. cereus spore) determination by immobilizing onto gold screen-printed electrodes a specific ssDNA aptamer as the recognition element and measuring the increase in R CT after binding B. cereus spores with the aptamer. The linear range for the determination extended from 10 4 to 10 6 cfu•mL −1 , and the LOD value was 3 × 10 3 cfu•mL −1 .…”

Section: Biological Weaponsmentioning

confidence: 99%

What Electrochemical Biosensors Can Do for Forensic Science? Unique Features and Applications

et al. 2019

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This article critically discusses the latest advances in the use of voltammetric, amperometric, potentiometric, and impedimetric biosensors for forensic analysis. Highlighted examples that show the advantages of these tools to develop methods capable of detecting very small concentrations of analytes and provide selective determinations through analytical responses, without significant interferences from other components of the samples, are presented and discussed, thus stressing the great versatility and utility of electrochemical biosensors in this growing research field. To illustrate this, the determination of substances with forensic relevance by using electrochemical biosensors reported in the last five years (2015–2019) are reviewed. The different configurations of enzyme or affinity biosensors used to solve analytical problems related to forensic practice, with special attention to applications in complex samples, are considered. Main prospects, challenges to focus, such as the fabrication of devices for rapid analysis of target analytes directly on-site at the crime scene, or their widespread use and successful applications to complex samples of interest in forensic analysis, and future efforts, are also briefly discussed.

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A label-free impedimetric aptasensor for the detection of Bacillus anthracis spore simulant

Cited by 62 publications

References 37 publications

Food Sensing: Detection of Bacillus cereus Spores in Dairy Products

Food Sensing: Detection of Bacillus cereus Spores in Dairy Products

Challenges in Electrochemical Aptasensors and Current Sensing Architectures Using Flat Gold Surfaces

What Electrochemical Biosensors Can Do for Forensic Science? Unique Features and Applications

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