A label-free impedimetric immunosensor based on covalent immobilization of anti-E. Coli antibody via a copper-catalyzed azide-alkyne cycloaddition reaction

Свалова, Т. С.; Medvedeva, M. V.; Saigushkina, Anna A.; Kozitsin, Ivan V.; Малышева, Н. Н.; Zhdanovskikh, V. O.; Охохонин, А. В.; Козицина, А. Н.

doi:10.1007/s00216-019-02381-1

Cited by 12 publications

(3 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Sensors for voltammetry have been improved. An original method of covalent immobilization of anti-E. coli antibodies on the surface of screen-printed carbon electrode by the copper-catalyzed azide-alkyne cycloaddition reaction was developed [114] (Figure 5). A polyvinylbenzylazide film was applied to the electrode surface by the electrochemical method with the simultaneous inclusion of copper-containing particles in the structure.…”

Section: The Use Of Physico-chemical Methods For the Development Of Immunosensorsmentioning

confidence: 99%

Antibodies as Biosensors’ Key Components: State-of-the-Art in Russia 2020–2021

Rudenko

Fursova

Shepelyakovskaya

et al. 2021

Sensors

View full text Add to dashboard Cite

The recognition of biomolecules is crucial in key areas such as the timely diagnosis of somatic and infectious diseases, food quality control, and environmental monitoring. This determines the need to develop highly sensitive display devices based on the achievements of modern science and technology, characterized by high selectivity, high speed, low cost, availability, and small size. Such requirements are met by biosensor systems—devices for reagent-free analysis of compounds that consist of a biologically sensitive element (receptor), a transducer, and a working solution. The diversity of biological material and methods for its immobilization on the surface or in the volume of the transducer and the use of nanotechnologies have led to the appearance of an avalanche-like number of different biosensors, which, depending on the type of biologically sensitive element, can be divided into three groups: enzyme, affinity, and cellular/tissue. Affinity biosensors are one of the rapidly developing areas in immunoassay, where the key point is to register the formation of an antigen–antibody complex. This review analyzes the latest work by Russian researchers concerning the production of molecules used in various immunoassay formats as well as new fundamental scientific data obtained as a result of their use.

show abstract

Section: The Use Of Physico-chemical Methods For the Development Of Immunosensorsmentioning

confidence: 99%

Antibodies as Biosensors’ Key Components: State-of-the-Art in Russia 2020–2021

Rudenko

Fursova

Shepelyakovskaya

et al. 2021

Sensors

View full text Add to dashboard Cite

show abstract

“…The inclusion of bio-nanomaterials in SPEs may occur according to various alternative strategies. Biological receptors, such as enzymes, nucleic acids, antibodies, or living cells, may be immobilized onto or on the SPE surface in different ways, such as casting, physical adsorption, or electrochemical coating [64][65][66]. Another method is the inclusion of the biological receptor into an ink, which is printed as a final layer on the electrode surface.…”

Section: Development Of Screen-printed Based Sensorsmentioning

confidence: 99%

Carbonaceous Nanomaterials Employed in the Development of Electrochemical Sensors Based on Screen-Printing Technique—A Review

Bounegru

Apetrei

2020

Catalysts

View full text Add to dashboard Cite

This paper aims to revise research on carbonaceous nanomaterials used in developing sensors. In general, nanomaterials are known to be useful in developing high-performance sensors due to their unique physical and chemical properties. Thus, descriptions were made for various structural features, properties, and manner of functionalization of carbon-based nanomaterials used in electrochemical sensors. Of the commonly used technologies in manufacturing electrochemical sensors, the screen-printing technique was described, highlighting the advantages of this type of device. In addition, an analysis was performed in point of the various applications of carbon-based nanomaterial sensors to detect analytes of interest in different sample types.

show abstract

“…Therefore, it is practical to create long-term storage precursors on the surface of the working electrode for subsequent one-step immobilization of the bio receptor. Previously, we developed an original method where copper catalyzed azide-alkyne cycloaddition (CuAAC) reaction on the screen-printed carbon electrode (SPE) surface, modified with electrodeposited polyvinyl benzyl azide (PVBA), was catalyzed by the products of electrochemical oxidation of copper particles incorporated in the structure of the polymer film [12]. It was successfully applied for covalent immobilization of anti-Escherichia coli (E. coli) antibodies and in construction of a label-free impedimetric immunosensor which have been demonstrated good analytical characteristics for E. coli detection (LOD 6.3 CFU/mL, a linear range 10 3 -10 6 CFU/mL) in model suspensions and real samples of milk and drinking water.…”

Section: Introductionmentioning

confidence: 99%

A “Clickable” Electrodeposited Polymer Films Based on 3‐Ethynylthiophene for the Covalent Immobilization of Proteins. Application to a Label‐free Electrochemical Immunosensor for Escherichia Coli and Staphylococcus Aureus Determination

2021

View full text Add to dashboard Cite

An original electrodeposited polymer film, based on 3-ethynylthiophene, for covalent immobilization of proteins via "click" reaction of copper catalyzed azidealkyne cycloaddition was developed. The best characteristics have demonstrated the layer-by-layer immobilized 3,4-ethylendioxythiophene-3-ethynylthiophene composite film. The bovine serum albumin and antibodies have been successfully immobilized on the surface of planar plati-num electrode. The approach has been applied to labelfree electrochemical immunosensors for Escherichia coli and Staphylococcus aureus determination. The immunosensors demonstrated LODs 7.2 CFU/mL for Escherichia coli and 15.9 CFU/mL for Staphylococcus aureus, linear range 10 2 -10 6 CFU/mL and could be promising for environmental and food control.

show abstract

A label-free impedimetric immunosensor based on covalent immobilization of anti-E. Coli antibody via a copper-catalyzed azide-alkyne cycloaddition reaction

Cited by 12 publications

References 22 publications

Antibodies as Biosensors’ Key Components: State-of-the-Art in Russia 2020–2021

Antibodies as Biosensors’ Key Components: State-of-the-Art in Russia 2020–2021

Carbonaceous Nanomaterials Employed in the Development of Electrochemical Sensors Based on Screen-Printing Technique—A Review

A “Clickable” Electrodeposited Polymer Films Based on 3‐Ethynylthiophene for the Covalent Immobilization of Proteins. Application to a Label‐free Electrochemical Immunosensor for Escherichia Coli and Staphylococcus Aureus Determination

Contact Info

Product

Resources

About