A laboratory high‐throughput glass chamber using dynamic headspace TD‐GC/MS method for the analysis of whole Brassica napus L. plantlet volatiles under cadmium‐related abiotic stress

Abstract

IntroductionThe dynamic headspace sampling technique using thermal desorption, gas chromatography‐mass spectrometry (TD‐GC/MS) is a powerful method for analysing plant emissions of volatile organic compounds (VOCs), and experiments performed in sterile and controlled conditions can be useful for VOC metabolism investigations.ObjectiveThe main purpose of this study was to set up a laboratory high‐throughput glass chamber for whole plant volatiles analysis. Brassica napus L. plantlets were tested with the developed system to better understand the relationship between low emission of induced terpene and cadmium (Cd)‐related abiotic stress.MethodologyVOCs emitted by 28‐day‐old Brassica napus L. plantlets cultivated in vitro were trapped with our device using adsorbent cartridges that were desorbed with a thermal desorption unit before cryofocusing with a cooled injection system and programmable temperature vaporising inlet into an HP‐5 ms GC column. Terpene detection and quantitation from chromatogram profiles were acquired using selected ion monitoring (SIM) mode during full scan analysis and mass spectra were obtained with a quadrupole‐type mass spectrometer.ResultsThe new trapping method produced reliable qualitative profiles of oilseed rape VOCs. Typical emissions of monoterpenes (myrcene, limonene) and sesquiterpenes (β‐elemene, (E,E)‐α‐farnesene) were found for the different concentrations tested. One‐way analysis of variance for quantitative results of (E,E)‐α‐farnesene emission rates showed a Cd concentration effect.ConclusionThis inexpensive glass chamber has potential for wide application in laboratory sterile approach and replicated research. Moreover, the non‐invasive dynamic sampling technique could also be used to analyse volatiles under both abiotic and biotic stresses.

Biopesticide Trunk Injection Into Apple Trees: A Proof of Concept for the Systemic Movement of Mint and Cinnamon Essential Oils

“…The TDU temperature program was 40°C for 1 min and was increased to 280°C at a rate of 100°C min –1 and held for 5 min. CIS was mounted with a baffled glass liner and operated in solvent vent mode, and the temperature program was -60°C for 0.10 min, which was increased to 250°C at a rate of 12°C s –1 and held for 2 min following existing protocols ( Delory et al, 2016 ; Durenne et al, 2018 ).…”

Encapsulation of Essential Oils for the Development of Biosourced Pesticides with Controlled Release: A Review

“…A second and strictly quantitative method to study the release is the use of gas chromatography coupled with mass spectrometry, in order to measure the quantity of EOs that can be released during a specific period of time. This method can be used with a static headspace (analysis of a sample of headspace) [57] or dynamic headspace (continued intake of gas flow, trapping on adsorbent cartridges, thermal desorption, and cryofocusing) [92].…”

Interactions between Brassica Biofumigants and Soil Microbiota: Causes and Impacts

“…102 Some of the volatile compounds emitted from GSL hydrolysis with inhibitory activity on plant protection potentials are ITCs, sesquiterpenes, and terpenes. 19,20 Upon decomposition, other than ITCs, nonglucosinolate breakdown products, such as dimethyl sulfide, dimethyl disulfide (DMDS), dimethyl trisulfide (DMTS), and allyl methyl disulfide (AMDS), are formed. 14 Bioactive compounds released from biofumigant species significantly affect the survival of soil-borne fungal pathogens (Table 4) through a direct fungicidal effect.…”