A LAMP–CRISPR/Cas12b rapid detection platform for canine parvovirus detection

Chen, Yuting; Zhang, Xinyu; Hu, Gui; Pan, Yueying; Guan, Yuhong; Yang, Jinquan; Chen, Hui

doi:10.1039/d4ay00977k

Anal. Methods

2024

DOI: 10.1039/d4ay00977k

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A LAMP–CRISPR/Cas12b rapid detection platform for canine parvovirus detection

Yuting Chen,

Xinyu Zhang,

Gui Hu

et al.

Abstract: Canine parvovirus (CPV) is one of the main pathogens causing toxic diarrhea in Chinese dogs, which is the cause of large-scale epidemic of dogs, and poses a great threat to...

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Cited by 2 publications

References 22 publications

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Biosensing platforms for DNA diagnostics based on CRISPR/Cas nucleases: towards the detection of nucleic acids at the level of single molecules in non-laboratory settings

Khmeleva,

Ptitsyn,

Kurbatov

et al. 2024

BIOMED KHIM

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The use of CRISPR/Cas nucleases for the development of DNA diagnostic systems in out-of-laboratory conditions (point-of-need testing, PONT) has demonstrated rapid growth in the last few years, starting with the appearance in 2017–2018 of the first diagnostic platforms known as DETECTR and SHERLOCK. The platforms are based on a combination of methods of nucleic acid isothermal amplification with selective CRISPR/Cas detection of target amplicons. This significantly improves the sensitivity and specificity of PONT, making them comparable with or even superior to the sensitivity and specificity of polymerase chain reaction, considered as the “gold standard” of DNA diagnostics. The review considers modern approaches to the coupling of CRISPR/Cas detection using Cas9, Cas12a, Cas12b, Cas13a, Cas14, and Cas3 nucleases to various methods of nucleic acid isothermal amplification, with an emphasis on works in which sensitivity at the level of single molecules (attomolar and subattomolar concentrations of the target) is achieved. The properties of CRISPR/Cas nucleases used for targeted DNA diagnostics and the features of methods of nucleic acid isothermal amplification are briefly considered in the context of the development of diagnostic biosensing platforms. Special attention is paid to the most promising directions for the development of DNA diagnostics using CRISPR/Cas nuclease.

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Biosensing platforms for DNA diagnostics based on CRISPR/Cas nucleases: towards the detection of nucleic acids at the level of single molecules in non-laboratory settings

Khmeleva,

Ptitsyn,

Kurbatov

et al. 2024

BIOMED KHIM

View full text Add to dashboard Cite

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Overview of Recent Advances in Canine Parvovirus Research: Current Status and Future Perspectives

Zhou,

Cui,

et al. 2024

Microorganisms

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Canine parvovirus (CPV-2) was first identified in the late 1970s and has since become one of the most significant infectious agents affecting dogs. CPV-2 causes severe diseases such as hemorrhagic gastroenteritis and myocarditis, posing a major threat to canine health, particularly with a high mortality rate in puppies. It is globally recognized as a highly contagious and lethal pathogen. CPV is prone to rapid mutation, leading to the emergence of new variants. Despite widespread vaccination efforts, CPV remains one of the primary causes of acute gastroenteritis and death in young and juvenile dogs. Furthermore, the detection of CPV in swine populations has introduced additional challenges to its control. This review summarizes the current epidemiological status of CPV, highlighting recent advancements in diagnostic techniques and vaccine development. Additionally, it discusses the latest research on the pathogenesis of the virus and the development of antiviral agent research and proposes prevention and control suggestions for CPV under the One Health concept. In particular, there is a need to enhance surveillance of viral dynamics, accelerate the development of novel vaccines, and deepen the exploration of the underlying pathogenic mechanisms. This review aims to provide a scientific foundation for effective control of CPV and to guide future research directions.

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A LAMP–CRISPR/Cas12b rapid detection platform for canine parvovirus detection

Abstract: Canine parvovirus (CPV) is one of the main pathogens causing toxic diarrhea in Chinese dogs, which is the cause of large-scale epidemic of dogs, and poses a great threat to...

Cited by 2 publications

References 22 publications

Biosensing platforms for DNA diagnostics based on CRISPR/Cas nucleases: towards the detection of nucleic acids at the level of single molecules in non-laboratory settings

Biosensing platforms for DNA diagnostics based on CRISPR/Cas nucleases: towards the detection of nucleic acids at the level of single molecules in non-laboratory settings

Overview of Recent Advances in Canine Parvovirus Research: Current Status and Future Perspectives

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