A Lanthanide‐Complex‐Based Ratiometric Luminescent Probe Specific for Peroxynitrite

Abstract

A lanthanide-complex-based ratiometric luminescence probe specific for peroxynitrite (ONOO(-)), 4'-(2,4-dimethoxyphenyl)-2,2':6',2''-terpyridine-6,6''-diyl]bis(methylenenitrilo)tetrakis(acetate)-Eu(3+)/Tb(3+) ([Eu(3+)/Tb(3+)(DTTA)]), has been designed and synthesized. Both [Eu(3+)(DTTA)] and [Tb(3+)(DTTA)] are highly water soluble with large stability constants at approximately 10(20), and strongly luminescent with luminescence quantum yields of 10.0 and 9.9%, respectively, and long luminescence lifetimes of 1.38 and 0.26 ms, respectively. It was found that the luminescence of [Tb(3+)(DTTA)] could be quenched by ONOO(-) rapidly and specifically in aqueous buffers, while that of [Eu(3+)(DTTA)] did not respond to the addition of ONOO(-). Thus, by simply mixing [Eu(3+)(DTTA)] and [Tb(3+)(DTTA)] in an aqueous buffer, a ratiometric luminescence probe specific for time-gated luminescence detection of ONOO(-) was obtained. The performance of [Tb(3+)(DTTA)] and [Eu(3+)/Tb(3+)(DTTA)] as the probes for luminescence imaging detection of ONOO(-) in living cells was investigated. The results demonstrated the efficacy and advantages of the new ratiometric luminescence probe for highly sensitive luminescence bioimaging application.

Study on the luminescence behavior of lanthanide ions with luminol by flow injection chemiluminescence analysis

“…The lanthanide family was divided into light lanthanides (LL) from La to Eu and heavy lanthanides (HL) from Gd to Lu. Among these ions, Eu III and Tb III with the luminescence activities were commonly acted as probes [4][5][6][7][8] in the chemical and biomedical analyses [9], such as DNA hybridization assay [10], cell imaging [11,12], in vivo sensing [13], protease activities detecting [14], drug delivery monitoring [15] and so forth. Based on the intrinsic fluorescence, the photochemical, photophysical and optical-magnetic properties of Ln III and current trends of lanthanide-based luminescent materials have arisen great attentions and were applied in telecommunications, LED devices and solar energy conversion were summarized by some important reviews [16][17][18][19] in recent years.…”

Luminescent lanthanide complexes with 4-acetamidobenzoate: Synthesis, supramolecular assembly via hydrogen bonds, crystal structures and photoluminescence

“…These make luminescent materials including Ln(III) ions very attractive for a variety of applications, such as effective light conversion devices, fluorescent labels and probes for high sensitive time-resolved fluorimetric immunoassays [6][7][8][9][10][11]. Since the f-f transitions are parityforbidden [4,5], free Ln(III) ions have low extinction coefficients leading to low luminescence intensity.…”

Utility of tris(4-bromopyridyl) europium complexes as versatile intermediates in the divergent synthesis of emissive chiral probes

“…[11][12][13][14][15][16][17][18][19] Recently, a series of very bright Eu(III) phosphinate and carboxylate complexes has been reported containing pyridyl-alkynyl-aryl chromphores (Fig. 1).…”