A Lectin Isolated from Bananas Is a Potent Inhibitor of HIV Replication

Swanson, Meghan R.; Winter, Harry C.; Goldstein, Irwin J.; Markovitz, David M.

doi:10.1074/jbc.m109.034926

Cited by 180 publications

(163 citation statements)

References 70 publications

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“…Like antibodies, some lectins are known to play important roles in the immune system including defense against invading microorganisms and modulation of inflammatory and autoreactive processes through specific recognition of cellular and/or bacterial surface saccharide moieties. These characteristics make lectins very useful for glycoanalysis and medicine development 16, 17, 18, 19, 20, 21. Due to their minimization of sample consumption and high throughput format for analyzing multiple targets simultaneously, lectin microarrays have been employed in the glycomics study 22, 23, 24, 25, 26.…”

Section: Introductionmentioning

confidence: 99%

Uncovering the Binding Specificities of Lectins with Cells for Precision Colorectal Cancer Diagnosis Based on Multimodal Imaging

et al. 2018

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There is a high desire for novel targets/biomarkers to diagnose and treat colorectal cancer (CRC). Here, an approach starting from a polyacrylamide hydrogel–based lectin microarray is presented to screen the high expression of glycans on the CRC cell surface and to identify new lectin biomarkers for CRC. Three common CRC cell lines (SW480, SW620, and HCT116) and one normal colon cell line (NCM460) are profiled on the microarray with 27 lectins. The experimental results reveal that CRC cells highly express the glycans with d‐galactose, d‐glucose, and/or sialic acid residues, and Uelx Europaeus Agglutinin‐I (UEA‐I) exhibits reasonable specificity with SW480 cells. After conjugation of UEA‐I with silica‐coated NaGdF4:Yb3+, Er3+@NaGdF4 upconversion nanoparticles, the follow‐up in vitro and in vivo experiments provide further evidence on that UEA‐I can serve as tumor‐targeting molecule to diagnose SW480 tumor by multimodal imaging including upconversion luminescence imaging, T 1‐weighted magnetic resonance imaging, and X‐ray computed tomography imaging.

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Section: Introductionmentioning

confidence: 99%

Uncovering the Binding Specificities of Lectins with Cells for Precision Colorectal Cancer Diagnosis Based on Multimodal Imaging

et al. 2018

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“…Peripheral blood mononuclear cells (PBMCs) were obtained by venipuncture from healthy donors, and monocytes were separated from peripheral blood lymphocytes (PBLs) by differential adhesion to plates as previously described (66). PBLs were washed three times with phosphate-buffered saline (PBS) and stimulated with 5 g/ml phytohemagglutinin (PHA-P; Sigma-Aldrich, St. Louis, MO) for 3 days in RPMI 1640 complete media containing 10% heatinactivated fetal bovine serum (FBS) and 10 U/ml of interleukin-2 (IL-2; Sigma-Aldrich, St. Louis, MO).…”

Section: Methodsmentioning

confidence: 99%

Regulation of the Human Endogenous Retrovirus K (HML-2) Transcriptome by the HIV-1 Tat Protein

Gonzalez-Hernandez

Cavalcoli

Sartor

et al. 2014

J Virol

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Approximately 8% of the human genome is made up of endogenous retroviral sequences. As the HIV-1 Tat protein activates the overall expression of the human endogenous retrovirus type K (HERV-K) (HML-2), we used next-generation sequencing to determine which of the 91 currently annotated HERV-K (HML-2) proviruses are regulated by Tat. Transcriptome sequencing of total RNA isolated from Tat-and vehicle-treated peripheral blood lymphocytes from a healthy donor showed that Tat significantly activates expression of 26 unique HERV-K (HML-2) proviruses, silences 12, and does not significantly alter the expression of the remaining proviruses. Quantitative reverse transcription-PCR validation of the sequencing data was performed on Tattreated PBLs of seven donors using provirus-specific primers and corroborated the results with a substantial degree of quantitative similarity. IMPORTANCEThe expression of HERV-K (HML-2) is tightly regulated but becomes markedly increased following infection with HIV-1, in part due to the HIV-1 Tat protein.The findings reported here demonstrate the complexity of the genome-wide regulation of HERV-K (HML-2) expression by Tat. This work also demonstrates that although HERV-K (HML-2) proviruses in the human genome are highly similar in terms of DNA sequence, modulation of the expression of specific proviruses in a given biological situation can be ascertained using next-generation sequencing and bioinformatics analysis.

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“…There is evidence that lectins found in green bananas could provide a potential treatment for HIV patients and other viral infections such as, Ebola and hepatitis [26][27][28]. Other parts of the banana plant also have potential use in medical foods.…”

Section: Green Bananas and Health Benefitsmentioning

confidence: 99%

Let’s Go Bananas! Green Bananas and their Health Benefits

Apostolopoulos

Antonipillai

Tangalakis

et al. 2017

Prilozi

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Bananas have enormous health benefits as a food for both animals and humans. They have been used as a complimentary medicine to treat pathological conditions since ancient times. Recently, there has been increased interest in the scientific validity of the beneficial effects of bananas in alleviating and treating disease conditions including, ulcers, infections, diabetes, diarrhea, colitis and blood pressure. Herein, we write on the potential therapeutic and functional benefits of certain species of bananas when consumed green as well as considering the properties of extracts from the non-fruit parts of the plant. We conclude that green bananas appear to deliver an array of health and therapeutic benefits

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A Lectin Isolated from Bananas Is a Potent Inhibitor of HIV Replication

Cited by 180 publications

References 70 publications

Uncovering the Binding Specificities of Lectins with Cells for Precision Colorectal Cancer Diagnosis Based on Multimodal Imaging

Uncovering the Binding Specificities of Lectins with Cells for Precision Colorectal Cancer Diagnosis Based on Multimodal Imaging

Regulation of the Human Endogenous Retrovirus K (HML-2) Transcriptome by the HIV-1 Tat Protein

Let’s Go Bananas! Green Bananas and their Health Benefits

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